基于星形金纳米颗粒修饰的81°倾斜光纤光栅生物传感器
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摘要
提出一种星形金纳米颗粒修饰的81°倾斜光纤光栅(TFG)生物传感器。通过金黄色葡萄球菌蛋白A固定法,将自制的高纯度新城疫病毒(NDV)单克隆抗体固定于81°TFG表面,制成对NDV具有特异性检测功能的生物传感器。结果表明,传感器对NDV的最低探测极限达10~25pg/mL,检测饱和点约为1 000pg/mL;在0~200pg/mL具有较好的线性相关度(R2约为0.911),相应的灵敏度约为1 394pm/(ng/mL)。此外,通过对NDV的特异性和临床性测试,表明该生物传感器对NDV具有高度的特异性和临床有效性。
A type of biosensor based on 81°tilted fiber grating(81°TFG)modified by star shaped Au nanoparticles(St-AuNP)was proposed.Highly purified newcastle disease virus(NDV) monoclonal antibodies(MAb) were immobilized on sensor surface by using staphylococcus aureus protein A(SPA)method,so as to form the biosensor specific to NDV.Experimental results show that,the proposed biosensor has a lowest detection limit(LOD)of10~25 pg/mL for NDV and become saturated at~1 000 pg/mL,and it has a linearity sensitivity of~1 394 pm/(pg/mL)with R2 of~0.911 in the low concentration range of 0~200 pg/mL.In addition,through the specificity and clinical test,the proposed biosensor shows very high specificity to NDV and clinical effectiveness.
A type of biosensor based on 81°tilted fiber grating(81°TFG)modified by star shaped Au nanoparticles(St-AuNP)was proposed.Highly purified newcastle disease virus(NDV) monoclonal antibodies(MAb) were immobilized on sensor surface by using staphylococcus aureus protein A(SPA)method,so as to form the biosensor specific to NDV.Experimental results show that,the proposed biosensor has a lowest detection limit(LOD)of10~25 pg/mL for NDV and become saturated at~1 000 pg/mL,and it has a linearity sensitivity of~1 394 pm/(pg/mL)with R2 of~0.911 in the low concentration range of 0~200 pg/mL.In addition,through the specificity and clinical test,the proposed biosensor shows very high specificity to NDV and clinical effectiveness.
引文
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[14]Sjoquist J,Meloun B,Hjelm H,et al.Protein A isolate from staphylococcus aureus after digestion with lysostaphin[J].Eur.J.Biochem.,1972,29(3):572-578.
[15]Kangwa M,Yelemane V,Polat A N,et al.High-level fedbatch fermentative expression of an engineered staphylococcal protein A based ligand in E.coli:purification and characterization[J].AMB Express,2015,5(1):70-79.
[2]Zhou K,Chen X,Zhang L,et al.Low thermal sensitivity grating devices based on Ex-45°tilting structure capable of forward-propagating cladding modes coupling[J].J.Lightwave Technol.,2006,24(12):5087-5094.
[3]Yan Z,Sun Z,Zhou K,et al.Numerical and experimental analysis of sensitivity-enhanced RI sensor based on 81°TFG in thin cladding fiber[J].J.Lightwave Technol.,2015,33(14):3023-3027.
[4]Chen X,Zhou K,Zhang L,et al.In-fiber twist sensor based on a fiber Bragg grating with 81°tilted structure[J].Photon.Technol.Lett.,2006,18(24):2596-2598.
[5]Mou C,Zhou K,Yan Z,et al.Liquid level sensor based on an excessively tilted fibre grating[J].Opt.Commun.,2013,30(1):271-275.
[6]罗彬彬,邹文根,赵明富,等.极大倾斜角度光纤光栅pH值传感器及其增敏研究[J].光学学报,2017,37(1):0106009-1-8.Luo B B,Zou W G,Zhao M F,et al.pH sensor based on fiber grating with extremely large tilt angle and its sensitivity enhancement[J].Act.Opt.Sin.,2017,37(1):0106009-1-8.
[7]Luo B,Yan Z,Sun Z,et al.Novel glucose sensor based on enzyme immobilized 81°tilted fiber grating[J].Opt.Express,2014,22(25):30571-30578.
[8]Luo B,Yan Z,Sun Z,et al.Biosensor based on excessively tilted fiber grating in thin-cladding optical fiber for sensitive and selective detection of low glucose concentration[J].Opt.Express,2015,23(25):32429-32440.
[9]Luo B,Wu S,Zou W,et al.Label-free immunoassay for porcine circovirus type 2 based on excessively tilted fiber grating modified with staphylococcal protein A[J].Biosensors and Bioelectron.,2016,86:1054-1060.
[10]Luo B,Wu S,Zhang Z,et al.Human heart failure biomarker immunosensor based on excessively tilted fiber gratings[J].Biomedical Opt.Express,2017,8(1):57-67.
[11]罗彬彬,吴胜昔,王玲玲,等.基于81°倾斜光纤光栅的新城疫病毒免疫传感器[J].光学学报,2017,37(11):1106001.Luo B B,Wu S X,Wang L L,et al.Study on newcastle disease virus immunosensor based on 81°tilted fiber grating[J].Act.Opt.Sin.,2017,37(11):1106001.
[12]Yan Z,Wang H,Wang C,et al.Theoretical and experimental analysis of excessively tilted fiber gratings[J].Opt.Express,2016,24(11):12107-12115.
[13]Zhang Q,Xue C,Yuan Y,et al.Fiber surface modification technology for fiber-optic localized surface plasmon resonance biosensors[J].Sensors,2012(12):2729-2741.
[14]Sjoquist J,Meloun B,Hjelm H,et al.Protein A isolate from staphylococcus aureus after digestion with lysostaphin[J].Eur.J.Biochem.,1972,29(3):572-578.
[15]Kangwa M,Yelemane V,Polat A N,et al.High-level fedbatch fermentative expression of an engineered staphylococcal protein A based ligand in E.coli:purification and characterization[J].AMB Express,2015,5(1):70-79.