卟啉多孔化合物的合成及其在光电免疫传感器中的应用
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摘要
构建了以卟啉多孔化合物-金纳米颗粒(Au NPs@PAF)为固定基质的无标记型C-反应蛋白(CRP)光电免疫传感器。在最优条件下,采用计时电流法实现了对CRP的定量检测。该传感器在CRP质量浓度0.05～60 ng/mL范围内与光电流有较好的线性关系,检出限为0.017 ng/mL,相关系数为0.994 6,平均回收率为102%,具有良好的选择性,为C-反应蛋白的检测提供了一种灵敏的方法。
In this paper,a label-free C-reactive protein(CRP)photoelectricimmunosensor was constructed using porous porphyrin compound-Au nanoparticles(Au NPs@PAF)as an immobilized substrate.Under the optimal conditions,the CRP was quantitatively detected by chronograph current method.There was a good linear relationship for the sensor versus photocurrent in the range of 0.05-60 ng/mL,with a linear correlation coefficient of 0.994 6.The limit of detection was 0.017 ng/mL,and the average recovery was 102%.This sensor provided a sensitive method for the detection of C-reactive protein with good selectivity.
In this paper,a label-free C-reactive protein(CRP)photoelectricimmunosensor was constructed using porous porphyrin compound-Au nanoparticles(Au NPs@PAF)as an immobilized substrate.Under the optimal conditions,the CRP was quantitatively detected by chronograph current method.There was a good linear relationship for the sensor versus photocurrent in the range of 0.05-60 ng/mL,with a linear correlation coefficient of 0.994 6.The limit of detection was 0.017 ng/mL,and the average recovery was 102%.This sensor provided a sensitive method for the detection of C-reactive protein with good selectivity.
引文
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[2] Meyer M,Hartmann M M.Biosens.Bioelectron.,2006,21(10):1987-1990.
[3] Gabay C,Kushner I.New Eng.J.Med.,1999,340(6):448-454.
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[5] Black S,Kushner I,Samols D.J.Biol.Chem.,2004,279(47):48487-48490.
[6] Tomita S,Nihon R.Jap.J.Clin.Med.,2000,162(17):2453-2456.
[7] Holownia P,Perezamodio S,Price C P.Anal.Chem.,2001,73(14):3426-3431.
[8] Hawker C D.Anal.Chem.,1973,45(11):878A-890A.
[9] Kurita R,Arai K,Nakamoto K,Kato D,Niwa O.Anal.Chem.,2010,82(5):1692-1697.
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[13] Zhang L,Mohamed H H,Dillert R,Bahnemann D.J.Photochem.Photobiol.C,2012,13(4):263-276.
[14] Zhang L.The Cell Photoelectrochemical Sensor Based on Porphyrin and 3-Aminophenylboronic Acid.Nanjing:Nanjing University(张龙.基于卟啉和3-氨基苯硼酸的细胞光电化学传感器研究.南京:南京大学),2012.
[15] Hu Y,Huang Y J,Wang Y Y,Li C Y,Wong W L,Ye X X,Sun D.Anal.Chim.Acta,2018,1035:136-145.
[16] Yang Z,Lei J,Ju H.Biosens.Bioelectron.,2017,96:8-16.
[17] Lu L M,Zhang X B,Kong R M.J.Am.Chem.Soc.,2011,133(30):11686-11691.
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[19] Du X,Dai L,Ding J,Li H,Hao N,You T Y,Mao H P,Wang K.Biosens.Bioelectron.,2017,91:706-713.
[20] Tan Y,Wang Y,Li M,Ye X X,Wu T,Li C.Biosens.Bioelectron.,2017,91:741-746.
[21] Cao H,Ye D,Zhao Q,Luo J,Zhang S,Kong J.Analyst,2014,139(19):4917-4923.
[22] Liu Y,Liu T Z,Hu R,Zhang K L,Bai R Y,Zheng L,Yang Y H. J.Yunnan Univ.:Nat.Sci.(刘仪,刘婷知,胡蓉,张坤蕾,白茹燕,郑丽,杨云慧.云南大学学报:自然科学版),2015,37(6):888-895.
[23] Li D L,Dai K,Zhang X,Zhang K L,Bai R Y,Hu R,Yang Y H.J.Yunnan Univ.:Nat.Sci.(李德蕾,戴坤,张茜,张坤蕾,白茹燕,胡蓉,杨云慧.云南大学学报:自然科学版),2017,39(3):447-453.
[24] Jampasa S,Siangproh W,Laocharoensuk R,Vilaivan T,Chailapakul O.Talanta,2018,183:311-319.
[25] Hu C,Zeimpekis I,Sun K,Anderson S,Ashburn P.Anal.Chem.,2016,88(9):4872-4878.
[26] Vashist S K,Schneider E M,Luong J H.Analyst,2015,140(13):4445-4452.