大豆β-Conglycinin对鱼类健康的影响及分离方法
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摘要
大豆抗原蛋白是限制大豆蛋白在水产饲料中广泛应用的主要因素。β-伴大豆球蛋白(β-Conglycinin)是免疫原性最强的大豆抗原蛋白,饲料中β-Conglycinin含量过高会导致鱼类生长速度下降、消化酶活力下降、肠道组织发生损伤,甚至死亡。本文概述β-Conglycinin的理化性质、对鱼类生长性能、肠道组织、消化酶活力、非特异性免疫指标和抗氧化能力的影响、分离提纯方法及去除工艺,为进一步研究大豆抗原蛋白的致敏机理提供参考,同时为合理开发利用大豆蛋白源提供依据。
Soybean antigen protein is a major factor limiting the widespread use of soy protein sources in aquafeeds. β-Conglycinin is the most immunogenic soybean antigen protein. The high content of β-Conglycinin in fish feed can result in decreased fish growth rate and digestive enzyme activity, intestinal damage, and even death. This paper summarized the physicochemical properties, separation and purification methods, removal process of β-Conglycinin and its effects on fish growth performance, intestinal tissue, digestive enzyme activity, non-specific immune index and antioxidant ability. This study provides reference for further research on the sensitisation mechanism of soybean antigenic protein, reasonable development and utilization of soybean protein source.
Soybean antigen protein is a major factor limiting the widespread use of soy protein sources in aquafeeds. β-Conglycinin is the most immunogenic soybean antigen protein. The high content of β-Conglycinin in fish feed can result in decreased fish growth rate and digestive enzyme activity, intestinal damage, and even death. This paper summarized the physicochemical properties, separation and purification methods, removal process of β-Conglycinin and its effects on fish growth performance, intestinal tissue, digestive enzyme activity, non-specific immune index and antioxidant ability. This study provides reference for further research on the sensitisation mechanism of soybean antigenic protein, reasonable development and utilization of soybean protein source.
引文
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[2] Meinlschmidt P,Schweiggert-Weisz U,Brode V,et al.Enzyme assisted degradation of potential soy protein allergens with special emphasis on the techno functionality and the avoidance of a bitter taste formation[J].LWT - Food Science and Technology,2016,68:707-716.
[3] Maruyama N,Adachi M,Takahashi K,et al.Crystal structures of recombinant and native soybean beta-Conglycinin beta homotrimers[J].European Journal of Biochemistry,2001,268(12):3595-3604.
[4] 方旭前,朱友林,邱丽娟.大豆过敏原与低过敏原种质创新[J].遗传,2006,28(8):1043-1050.(Fang X Q,Zhu Y L,Qiu L J.Soybean allergens and hypoallergenic germplasm enhancement[J].Hereditas,2006,28(8):1043-1050.)
[5] Yue H,Zhen F Z,Peng F G,et al.Soybean β-Conglycinin induced gut hypersensitivity reaction in a piglet model[J].Archives of Animal Nutrition,2009,63(3):188-202.
[6] 孙泽威,秦贵信,张庆华.大豆抗原蛋白对犊牛生长性能、日粮养分消化率和肠道吸收功能的影响[J].中国畜牧杂志,2005,41(11):30-33.(Sun Z W,Qin G X,Zhang Q H.Effects of soy antigen protein on growth performance,dietary nutrient digestibility and intestinal absorption function of calves[J].Chinese Journal of Animal Science,2005,41(11):30-33.)
[7] Johnston C.Effect of injecting lambs with soy flour extract on serum soy protein antibody concentration and rate of gain[J].Small Ruminant Research,1996,21(2):149-154.
[8] Christensen H R,Bruun S W ,Frokiaer H.Antigenic specificity of serum antibodies in mice fed soy protein[J].International Archives of Allergy and Applied Immunology,2003,132(1):58-67.
[9] 吴莉芳,吴亚男,王洪鹤,等.大豆主要抗原蛋白对鲤鱼肌肉营养成分的影响[J].大豆科学,2010,29(1):105-108.(Wu L F,Wu Y N,Wang H H,et al.Effects of main soybean antigenon nutritional componentsin muscle of carp(Cyprinus carpio)[J].Soybean Science,2010,29(1):105-108.)
[10] 吴莉芳,孙泽威,秦贵信,等.Glycinin和β-Conglycinin对不同食性鱼类生长及肠道组织的影响[J].西北农林科技大学学报(自然科版),2011,39(2):59-66,74.(Wu L F,Sun Z W,Qin G X,et al.Effects of dietary Glycinin and β-Conglycinin on growth performance and intestinal tissue of fishes with different food habits[J].Journal of Northwest A& F University(Nature Science Edition) ,2011,39(2):59-66,74.)
[11] Rumsey G L,Siwicki A K,Anderson D P,et al.Effect of soybean protein on serological response,non-specific defense mechanisms,growth,and protein utilization in rainbow trout[J].Veterinary Immunology and Immunopathology,1994,41(3-4):323-329.
[12] 谷珉.影响海水鱼虾对植物蛋白利用的抗营养因子和蛋氨酸的研究[D].青岛:中国海洋大学,2013.(Gu M.The research on the plant protein utilization by marine fish and shrimp anti-nutritional factors and methionine[D].Qingdao:Ocean University of China,2013.)
[13] Zhang J X,Guo L Y,Feng L,et al.Soybean β-Conglycinin induces inflammation and oxidation and causes dysfunction of intestinal digestion and absorption in fish[J].Plos One,2013,8(3):e58115.
[14] Xiang P,Haas E J,Zeece M G,et al.C-terminal 23 kDa polypeptide of soybean Gly m Bd 28 K is a potential allergen[J].Planta,2004,220(1):56-63.
[15] Mujoo R,Trinh D T,Ng P K W,et al.Characterization of storage proteins in different soybean varieties and their relationship to tofu yield and texture[J].Food Chemistry,2003,82(2):265-273.
[16] Maruyama N,Fukuda T,Saka S,et al.Molecular and structural analysis of electrophoretic variants of soybean seed storage proteins[J].Phytochemistry 2003,64(3):701-708.
[17] Amigobenavent M,Clemente A,Castillo M D D,et al.Digestibility and immunore activity of soybean β-Conglycinin and its deglycosylated form[J].Food Chemistry,2011,129(4):1598-1605.
[18] Krishnan H B,Kim W S,Jang S,et al.All three subunits of soybean β-Conglycinin are potential food allergens[J].Journal of Agricultural and Food Chemistry,2009,57(3):938-943.
[19] Coates J B,Medeiros J S,Thanh V H,et al.Characterization of the subunits of beta-Conglycinin[J].Archives of Biochemistry & Biophysics,1985,243(1):184-194.
[20] Zhao Y,Qin G X,Sun Z W,et al.Effects of glycinin and β-Conglycinin on enterocyte apoptosis,proliferation and migration of piglets[J].Food and Agricultural Immunology,2010,21(3):209-218.
[21] Hao Y,Zhan Z F,Guo P F,et al.Soybean β-Conglycinin-induced gut hypersensitivity reaction in a piglet model[J].Archives of Animal Nutrition,2009,63(3):188-202.
[22] Han P,Ma X,Yin J,et al.The effects of lipoic acid on soybean β-Conglycinin-induced anaphylactic reactions in a rat model[J].Archives of Animal Nutrition,2010,64(3):254-264.
[23] Li Y,Hu H,Liu J,et al.Dietary soya allergen β-Conglycinin induces intestinal inflammatory reactions,serum-specific antibody response and growth reduction in a carnivorous fish species,turbot Scophthalmus maximus L.[J].Aquaculture Research,2017,48.
[24] 吴莉芳,邹瑞兴,王申,等.大豆主要抗原蛋白对埃及胡子鲇肌肉营养成分的影响[J].吉林农业大学学报,2009,31(6):741-745.(Wu L F,Zou R X,Wang S,et al.Effects of main soybean antigens on nutritional components of Musclein Clarias leather[J].Journal of Jilin Agricultural University,2009,31(6):741-745.)
[25] 吴莉芳,吴亚男,周敏,等.大豆抗原蛋白对草鱼肌肉营养成分的影响[J].吉林农业大学学报,2010,32(2):214-217,220.(Wu L F,Wu Y N,Zhou M,et al.Effects of main soybean antigen on nutritional components in muscle of Ctenopharyngodon idellus[J].Journal of Jilin Agricultural University,2010,32(2):214-217,220.)
[26] 刘襄河,叶继丹,王子甲,等.饲料中豆粕替代鱼粉比例对牙鲆生长性能及生化指标的影响[J].水产学报,2010,34(3):450-458.(Liu X H,Ye J D,Wang Z S,et al.Partial replacement of fish meal by soybean meal in diets for juvenile Japanese flounder[J].Journal of Fisheries of China,2010,34(3):450-458.)
[27] Guo P,Piao X,Cao Y,et al.Recombinant soybean protein beta-Conglycinin alpha-subunit expression and induced hypersensitivity reaction in rats[J].International Archives of Allergy and Immunology,2008,145:102-110.
[28] Gu M,Bai N,Xu W,et al.Effects of dietary β-Conglycinin and glycinin on digestive enzymes activities,intestinal histology and immune responses of juvenile turbot Scophthalmus maximus[J].Aquaculture Research,2016,47(3):1001-1008.
[29] 米海峰,孙瑞健,张璐,等.鱼类肠道健康研究进展[J].中国饲料,2015(15):19-22.(Mi H F,Sun R J,Zhang L,et al.Advances in research on fish intestinal health[J].China Feed,2015(15):19-22.)
[30] Taher S,Romano N P,Arshad A,et al.Assessing the feasibility of dietary soybean meal replacement for fishmeal to the swimming crab,Portunus pelagicus,juveniles[J].Aquaculture,2017,469:88-94.
[31] Han F L,Wang X D,Guo J L,et al.Effects of glycinin and β-Conglycinin on growth performance and intestinal health in juvenile Chinese mitten crabs (Eriocheir sinensis)[J].Fish and Shellfish Immunology,2019(84):269-279.
[32] 邢秀苹,赖红娥,赵晗,等.β-Conglycinin对不同发育时期鲤鱼消化酶活力的影响[J].西北农林科技大学学报(自然科学版),2016,44(1):19-24,30.(Xing X P,Lai H E,Zhao H,et al.Effect of β-Conglycinin on activities of protease and amylease in juvenile and larval common carps[J].Journal of Northwest A&F University(Natural Science Edition),2016,44(1):19-24,30.)
[33] 孙玲.大豆抗原蛋白对不同食性鱼类消化酶活性及血液指标的影响[D].长春:吉林农业大学,2008.(Sun L.Effeets of soybean antigen on the activities of digestive enzyme and blood index of different fishes[D].Changchun:Jilin Agricultural University,2008.)
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