枸杞多糖免疫增效剂对滩羊抗体水平和细胞因子分泌的影响
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摘要
研究枸杞多糖免疫增效剂对滩羊机体免疫力的影响,选择体况相近滩羊20只,每天饲喂同样的饲草料,同时每日添加枸杞多糖免疫增效剂为0.5g/只,分别在试验开始前、7d、14d后各采血一次,利用定量检测卡检测滩羊免疫因子IgG、IgM、IgA、IL-2、IL-13、IL-9、IL-17、IFN-γ、TNF-β等9项指标,并用血常规分析仪分析白细胞、淋巴细胞。结果表明,IgA、IgM、IgG含量在饲喂7d和14d都有增长趋势,IL-2、IL-13、IL-17、IL-19在饲喂后含量有上升的趋势,TNF-α、IFN-γ饲喂后含量均升高,说明枸杞多糖免疫增效剂饲喂7d,可提高滩羊抗体水平和细胞因子分泌。
This work mainly focused on studying the effects of Lycium barbarum polysaccharides(LBP)immuneopotentiator on the immunity in Tan sheep.20 Tan sheep with similar body condition were chosen,and were fed with the same forage daily.At the same time,o.5 g LBP was added to each sheep per day.The blood was taken from the sheep before the test,7 days and 14 days after the test respectively.IgG,IgM,IgA,IL-2,IL-13,IL-9,IL-17,IFN-γ,TNF-βlevels were tested by the quantitative detection card,and the white blood cells and lymphocytes were analyzed by blood routine analyzer.The results showed that the contents of IgA,IgM and IgG all increased both after feeding for 7 days and 14 days.The contents of IL-2,IL-13,IL-17 and IL-19 had a tendency of increase after feeding.The contents of TNF-αand IFN-γall increased after feeding.Taken together,the immunity of Tan sheep was remarkably improved by administration with LBP immuneopotentiator for 7 days.
This work mainly focused on studying the effects of Lycium barbarum polysaccharides(LBP)immuneopotentiator on the immunity in Tan sheep.20 Tan sheep with similar body condition were chosen,and were fed with the same forage daily.At the same time,o.5 g LBP was added to each sheep per day.The blood was taken from the sheep before the test,7 days and 14 days after the test respectively.IgG,IgM,IgA,IL-2,IL-13,IL-9,IL-17,IFN-γ,TNF-βlevels were tested by the quantitative detection card,and the white blood cells and lymphocytes were analyzed by blood routine analyzer.The results showed that the contents of IgA,IgM and IgG all increased both after feeding for 7 days and 14 days.The contents of IL-2,IL-13,IL-17 and IL-19 had a tendency of increase after feeding.The contents of TNF-αand IFN-γall increased after feeding.Taken together,the immunity of Tan sheep was remarkably improved by administration with LBP immuneopotentiator for 7 days.
引文
[1]杨宇为.不同添加剂对舍饲滩羊肉品质调控及生产性能影响的研究[D].宁夏银川:宁夏大学,2015.
[2]中华人民共和国药典:二部[M].北京:中国医药出版社,2015:365.
[3]Kevin K P,Joseph W L,Jackie N C,et al.Protective effect of Lycium barbarum polysaccharides on dextromethorphan-induced mood impairment and neurogenesis suppression[J].Brain Res Bulletin,2017,134(9):10-17.
[4]刘英娟,杜金梁,贾睿,等.枸杞多糖对四氯化碳致建鲤急性肝损伤的保护作用[J].浙江农业学报,2015,27(1):37-43.
[5]Yang M,Ding J,Zhou X,et al.Effects of lycium barbarum polysaccharides on neuropeptide Y and heat-shock protein 70expression in rats exposed to heat[J].Cancer Biother Radio Pharm,2014,(5):687-692.
[6]王建东,马吉锋,李艳艳,等.枸杞多糖对动物免疫系统的影响[J].黑龙江畜牧兽医2014(15):66-68.
[2]中华人民共和国药典:二部[M].北京:中国医药出版社,2015:365.
[3]Kevin K P,Joseph W L,Jackie N C,et al.Protective effect of Lycium barbarum polysaccharides on dextromethorphan-induced mood impairment and neurogenesis suppression[J].Brain Res Bulletin,2017,134(9):10-17.
[4]刘英娟,杜金梁,贾睿,等.枸杞多糖对四氯化碳致建鲤急性肝损伤的保护作用[J].浙江农业学报,2015,27(1):37-43.
[5]Yang M,Ding J,Zhou X,et al.Effects of lycium barbarum polysaccharides on neuropeptide Y and heat-shock protein 70expression in rats exposed to heat[J].Cancer Biother Radio Pharm,2014,(5):687-692.
[6]王建东,马吉锋,李艳艳,等.枸杞多糖对动物免疫系统的影响[J].黑龙江畜牧兽医2014(15):66-68.