癃清片体外抗菌活性的初步研究
|
摘要
【目的】研究癃清片不同极性部位对临床常见致病菌的体外抗菌活性。【方法】采用琼脂扩散法进行体外抑菌试验,微量稀释法测定最低抑菌浓度(minimum inhibitory concentration,MIC)、最低杀菌浓度(minimum bactericidal concentration,MBC),比较癃清片不同极性部位的体外抗菌活性。【结果】癃清片各极性部位呈现不同程度的抑菌作用,其中对金黄色葡萄球菌和大肠埃希菌呈高度敏感。癃清片正丁醇部位对金黄色葡萄球菌抑菌圈为12.5 mm,MIC为3.13 mg/ml,MBC为6.25 mg/ml;大肠埃希菌抑菌圈均大于8.2 mm,MIC为12.5 mg/ml,MBC为25 mg/ml。【结论】癃清片正丁醇部位对金黄色葡萄球菌和大肠埃希菌具有显著的抗菌效果,为进一步临床研究提供科学依据。
【Objective】To study the in vitro antibacterial activities of different extractions from Longqing Tablet against common clinical pathogens.【Methods】The in vitro antibacterial activity was evaluated by agar-diffusion method. Microdilution method was used to determine the minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC). The in vitro antibacterial effects were compared in different extractions of Longqing Tablet.【Results】The different extractions of Longqing Tablet showed different antibacterial activities. It was highly sensitive to Staphylococcus aureus and Escherichia coli. The inhibition zone of N-butyl alcohol extract to Staphylococus aureus was 12.5 mm, and MIC and MBC were respectively 3.13 mg/ml and 6.25 mg/ml. Its inhibition zone to Escherichia coli was 8.2 mm, and MIC and MBC were respectively 12.5 mg/ml and 25 mg/ml.【Conclusion】The N-butyl alcohol extraction of Longqing Tablet has strong antimicrobial activity against Staphylococus aureus and Escherichia coli. It will provide a scientific basis for the further clinical application.
【Objective】To study the in vitro antibacterial activities of different extractions from Longqing Tablet against common clinical pathogens.【Methods】The in vitro antibacterial activity was evaluated by agar-diffusion method. Microdilution method was used to determine the minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC). The in vitro antibacterial effects were compared in different extractions of Longqing Tablet.【Results】The different extractions of Longqing Tablet showed different antibacterial activities. It was highly sensitive to Staphylococcus aureus and Escherichia coli. The inhibition zone of N-butyl alcohol extract to Staphylococus aureus was 12.5 mm, and MIC and MBC were respectively 3.13 mg/ml and 6.25 mg/ml. Its inhibition zone to Escherichia coli was 8.2 mm, and MIC and MBC were respectively 12.5 mg/ml and 25 mg/ml.【Conclusion】The N-butyl alcohol extraction of Longqing Tablet has strong antimicrobial activity against Staphylococus aureus and Escherichia coli. It will provide a scientific basis for the further clinical application.
引文
[1]郭晓焕.泌尿系统微生物检验病原菌的结果探讨[J].中国现代药物应用,2016,10(5):20-21.
[2]孟晶茜,王建生,赵瑛瑛,等.尿路感染分离金黄色葡萄球菌的耐药性研究[J].中国病原生物学杂志,2017,12(2):165-168.
[3]陈潇雨,屈颖伟,王锁刚,等.不同剂量癃清片治疗上、下尿路感染临床研究[J].中医学报,2016,31(6):899-901.
[4]代宏亮,贾玉森,陈小均,等.癃清片预防膀胱镜检术后下尿路感染的临床研究[J].中国临床药理学杂志,2016,32(9):795-796.
[5]夏康,庞然,莫君甫,等.癃清片治疗ⅢB型慢性前列腺炎伴少弱精症的临床效果[J].广西医学,2018,40(15):1653-1655.
[6]加尔肯·热合木拜,地力木拉提·奴拉合买提.癃清片联合五水头孢唑林钠对老年肾结石合并反复尿路感染的疗效及对α1-MG、β2-MG、细胞面条的影响[J].西北国防医学杂志,2018,39(8):540-544.
[7]巩楠,段丽萍,郑丽霞,等.癃清片治疗老年女性下尿路感染的疗效观察[J].实用心脑肺血管病杂志,2010,18(10):1486-1487.
[8]陈彤,刘大乐,刘增钦,等.坦索罗辛联合癃清片治疗前列腺增生并逼尿肌无力临床研究[J].实验中医药杂志,2009,25(5):310-311.
[9]唐明茹,苏婕,石广梅.癃清片对小鼠体内抗菌作用的研究[J].天津药效,1994,6(4):15-17.
[2]孟晶茜,王建生,赵瑛瑛,等.尿路感染分离金黄色葡萄球菌的耐药性研究[J].中国病原生物学杂志,2017,12(2):165-168.
[3]陈潇雨,屈颖伟,王锁刚,等.不同剂量癃清片治疗上、下尿路感染临床研究[J].中医学报,2016,31(6):899-901.
[4]代宏亮,贾玉森,陈小均,等.癃清片预防膀胱镜检术后下尿路感染的临床研究[J].中国临床药理学杂志,2016,32(9):795-796.
[5]夏康,庞然,莫君甫,等.癃清片治疗ⅢB型慢性前列腺炎伴少弱精症的临床效果[J].广西医学,2018,40(15):1653-1655.
[6]加尔肯·热合木拜,地力木拉提·奴拉合买提.癃清片联合五水头孢唑林钠对老年肾结石合并反复尿路感染的疗效及对α1-MG、β2-MG、细胞面条的影响[J].西北国防医学杂志,2018,39(8):540-544.
[7]巩楠,段丽萍,郑丽霞,等.癃清片治疗老年女性下尿路感染的疗效观察[J].实用心脑肺血管病杂志,2010,18(10):1486-1487.
[8]陈彤,刘大乐,刘增钦,等.坦索罗辛联合癃清片治疗前列腺增生并逼尿肌无力临床研究[J].实验中医药杂志,2009,25(5):310-311.
[9]唐明茹,苏婕,石广梅.癃清片对小鼠体内抗菌作用的研究[J].天津药效,1994,6(4):15-17.