新分离植物乳杆菌的药敏性和抑菌性试验
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摘要
从市售泡菜、酸奶、肉牛牦牛粪便以及天然植物等基质中新分离出8株植物乳杆菌,采用K-B法和双层琼脂扩散法评价这8株植物乳杆菌对13种常用抗生素的耐药性以及对14株致病菌株的抗菌活性。结果表明,不同来源所分离植物乳杆菌的敏感性基本相似,对β-内酰胺类抗生素敏感,但对万古霉素、氨基糖苷类和喹诺酮类等临床上常见抗生素均有抗性。这8株植物乳杆菌对12株指示菌有不同程度的抑制作用,对强致病性大肠杆菌、鼠伤寒沙门菌和金黄色葡萄球菌等的抑菌功能较强。这些具有良好抗菌性的植物乳杆菌具有防治动物胃肠道疾病的潜能,但仍需对其益生潜能进行深入研究。
Eight Lactobacillus plantarum strains were isolated from kimchi,yoghurt,feces of cattle or yaks and natural plants.The K-B method and the double-layer agar diffusion method were used to evaluate the antibiotic susceptibility of eight L.plantarum strains and the antimicrobial activity against fourteen strains of pathogenic bacteria.The results indicated that the antibiotic resistance of eight L.plantarum strains isolated from different sources was almost same and sensitive to β-lactams,but highly resistant to clinically important antibiotics,including vancomycin, aminoglycosides and quinolones.The eight L.plantarum strains showed different degrees of inhibition on twelve pathogenic bacteria,and exhibited strong antimicrobial activity against Escherichia coli,Salmonella typhimurium and Staphylococcus aureus,and so on.These L.plantarum strains with high antimicrobial activity and antibiotic resistance may be useful for controlling gastrointestinal diseases in animals,but the probiotic potential of these strains still needs to be further studied.
Eight Lactobacillus plantarum strains were isolated from kimchi,yoghurt,feces of cattle or yaks and natural plants.The K-B method and the double-layer agar diffusion method were used to evaluate the antibiotic susceptibility of eight L.plantarum strains and the antimicrobial activity against fourteen strains of pathogenic bacteria.The results indicated that the antibiotic resistance of eight L.plantarum strains isolated from different sources was almost same and sensitive to β-lactams,but highly resistant to clinically important antibiotics,including vancomycin, aminoglycosides and quinolones.The eight L.plantarum strains showed different degrees of inhibition on twelve pathogenic bacteria,and exhibited strong antimicrobial activity against Escherichia coli,Salmonella typhimurium and Staphylococcus aureus,and so on.These L.plantarum strains with high antimicrobial activity and antibiotic resistance may be useful for controlling gastrointestinal diseases in animals,but the probiotic potential of these strains still needs to be further studied.
引文
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[3]马千里,刘冬,顾瑞霞.植物乳杆菌的益生特性及其在乳制品中的应用[J].中国奶牛,2014(1):36-40.MA Qian-li,LIU Dong,GU Rui-xia.Probiotic properties of Lactobacillus plantarum and its application in dairy products[J].China Dairy Cattle,2014(1):36-40.(in Chinese)
[4]SENOK A C,ISMAEEL A Y,BOTTA G A.Probiotics:facts and myths[J].Clinical Microbiology&Infection,2005,11(12):958-966.
[5]李雪.Lactobacillus plantarum KLDS1.0391和KLDS1.0706益生潜能的体外评价[D].哈尔滨:东北农业大学,2015:13-14.LI Xue.Probiotic potential in vitro of Lactobacillus plantarum KLDS1.0391 and KLDS1.0706[D].Harbin:Northeast Agricultural University,2015:13-14.(in Chinese)
[6]BRONZWAER S L A M,CARS O,BUCHHOLZ U,et al.A European study on the relationship between antimicrobial use and antimicrobial resistance[J].Emerging Infectious Diseases,2002,8(3):278-282.
[7]BELLETTI N,GATTI M,BOTTARI B,et al.Antibiotic resistance of lactobacilli isolated from two Italian hard cheeses[J].Journal of Food Protection,2009,72(10):2162-2169.
[8]QIAO M,YING G G,SINGER A C,et al.Review of antibiotic resistance in China and its environment[J].Environment International,2018,110:160-172.
[9]DELCOUR J,FERAIN T,DEGHORAIN M,et al.The biosynthesis and functionality of the cell-wall of lactic acid bacteria[J].Antonie Van Leeuwenhoek,1999,76(1-4):159-184.
[10]MATHUR S,SINGH R.Antibiotic resistance in food lactic acid bacteria-a review[J].International Journal of Food Microbiology,2005,105(3):281-295.
[11]CLEMENTI F,AQUILANTI L.Recent investigations and updated criteria for the assessment of antibiotic resistance in food lactic acid bacteria[J].Anaerobe,2011,17(6):394-398.
[12]HUMMEL A S,HERTEL C,HOLZAPFEL W H,et al.Antibiotic resistances of starter and probiotic strains of lactic acid bacteria[J].Applied and Environmental Microbiology,2007,73(3):730-739.
[13]YAACOB S N S,HUYOP F,IBRAHIM R K R,et al.Identification of Lactobacillus spp.and Fructobacillus spp.isolated from fresh Heterotrigona itama honey and their antagonistic activities against clinical pathogenic bacteria[J].Journal of Apicultural Research,2018,57(3):395-405.
[14]DIMITONOVA S P,DANOVA S T,SERKEDJIEVA J P,et al.Antimicrobial activity and protective properties of vaginal lactobacilli from healthy Bulgarian women[J].Anaerobe,2007,13(5-6):178-184.
[15]MALDONADO N C,RUIZ C S D,OTERO M C,et al.Lactic acid bacteria isolated from young calves-characterization and potential as probiotics[J].Research in Veterinary Science,2012,92(2):342-349.
[16]JABBARI V,KHIABANI M S,MOKARRAM R R,et al.Lactobacillus plantarum as a probiotic potential from Kouzeh cheese(Traditional Iranian Cheese)and its antimicrobial activity[J].Probiotics&Antimicrobial Proteins,2017,9(2):189-193.
[17]PEDERSEN C,ROOS S.Lactobacillus saerimneri sp.nov.isolated from pig faeces[J].International Journal of Systematic&Evolutionary Microbiology,2004,54(Pt 4):1365-1368.
[18]LEMA M,WILLIAMS L,RAO D R.Reduction of fecal shedding of enterohemorrhagic Escherichia coli O157:H7 in lambs by feeding microbial feed supplement[J].Small Ruminant Research,2001,39(1):31-39.
[19]DOWARAH R,VERMA A K,AGARWAL N,et al.Selection and characterization of probiotic lactic acid bacteria and its impact on growth,nutrient digestibility,health and antioxidant status in weaned piglets[J].PLoS One,2018,13(3):e192978.
[20]KAEWNOPPARAT S,DANGMANEE N,KAEWNOPPARAT N,et al.In vitro probiotic properties of Lactobacillus fermentum SK5 isolated from vagina of a healthy woman[J].Anaerobe,2013,22:6-13.