仙龙颗粒对H_(22)移植瘤小鼠免疫调节作用的实验研究
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摘要
目的:探讨仙龙颗粒对H_(22)移植瘤小鼠的免疫调节作用。方法:建立小鼠肝癌H_(22)移植瘤动物模型,小鼠随机分为5组,模型组、转移因子(20 mL/kg)对照组及仙龙颗粒(4、2、1 g/kg)组,每组14只;每日灌胃给予1次,连续给药10 d。通过测定小鼠胸腺、脾脏指数及巨噬细胞吞噬功能、淋巴细胞转化功能、NK细胞活性观察仙龙颗粒的免疫调节作用。结果:仙龙颗粒4、2 g/kg剂量组可明显提高移植瘤小鼠胸腺指数(P <0. 05),显著提高移植瘤小鼠腹腔巨噬细胞吞噬功能(P <0. 01,P <0. 05); 4 g/kg组能够明显促进ConA介导的T淋巴细胞转化能力(P <0. 05)、明显提高NK细胞的杀伤活性(P <0. 05)。结论:仙龙颗粒具增强肝癌H_(22)移植瘤小鼠免疫功能的作用。
Objective: To investigate the immunomodulatory effect of Xianlong Granule on the H_(22) transplanted tumor mice. Methods: To establish murine hepatocellular carcinoma H_(22) xenograft animal model,models was divided into model,transfer factor( 20 mL/kg) positive control and Xianlong Granule( 4,2,1 g/kg) groups,daily intragastric administration once time for 10 days,mice's thymus and spleen indexes,macrophage phagocytosis,lymphocyte transformation function and NK cell activity were determined. Results: The thymus index of Xianlong Granule 4,2 g/kg groups was increased( P < 0. 05),macrophage phagocytosis was raised,T lymphocyte transformation function of Xianlong Granule 4 g/kg group was promoted and NK cell activity was enhanced( P < 0. 05). Conclusion: Xianlong Granule can strengthen immunologic function of transplanted tumor mice.
Objective: To investigate the immunomodulatory effect of Xianlong Granule on the H_(22) transplanted tumor mice. Methods: To establish murine hepatocellular carcinoma H_(22) xenograft animal model,models was divided into model,transfer factor( 20 mL/kg) positive control and Xianlong Granule( 4,2,1 g/kg) groups,daily intragastric administration once time for 10 days,mice's thymus and spleen indexes,macrophage phagocytosis,lymphocyte transformation function and NK cell activity were determined. Results: The thymus index of Xianlong Granule 4,2 g/kg groups was increased( P < 0. 05),macrophage phagocytosis was raised,T lymphocyte transformation function of Xianlong Granule 4 g/kg group was promoted and NK cell activity was enhanced( P < 0. 05). Conclusion: Xianlong Granule can strengthen immunologic function of transplanted tumor mice.
引文
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[9]冯蕾,杨宪勇,冀海伟,等.北沙参超临界二氧化碳萃取物对免疫抑制小鼠免疫功能的影响及成分分析[J].中国医院药学杂志,2010,30(20):1740-1742.
[10]李英辉,张晶,孙莹,等.莪术提取液体内外抗肿瘤作用的实验研究[J].实用肿瘤医学杂志,2010,24(4):368-369.
[11]Malhotra A,Shanker A. NK cells:immune cross-talk and therapeutic implications[J]. Immunotherapy,2011,3(1):1143-1166.
[2]易清清,谭莹,李曰,等.“益元抑瘤汤”对H22荷瘤小鼠免疫功能影响的实验研究[J].中国免疫学杂志,2014,30(2):205-208.
[3]王学习,路莉,王敏,等.糙叶败酱大孔吸附树脂提取物对S180荷瘤小鼠免疫功能的影响[J].中药药理与临床,2012,28(3):71-74.
[4]骆苏芳,黄俊明.益元口服液对正常小鼠免疫功能的影响[J].第一军医大学学报,2005,25(8):1012-1015.
[5]陆平成,许益民.半枝莲多糖对细胞免疫的调节作用[J].南京中医学院学报,1989,20(2):32-39.
[6]瞿俊勇,田梦,贺建华,等.白花蛇舌草多糖对免疫抑制小鼠的免疫调节作用研究[J].中药材,2015,38(9):1942-1945.
[7]康林之,袁敏,邵峰,等.仙鹤草不同提取物抗疲劳抗氧化及免疫调节作用研究[J].时珍国医国药,2016,27(5):1079-1081.
[8]蔡晶,李孝栋,陈旭征,等.太子参多糖粗提物对小鼠免疫功能的影响[J].福建中医学院学报,2005,15(3):33-35.
[9]冯蕾,杨宪勇,冀海伟,等.北沙参超临界二氧化碳萃取物对免疫抑制小鼠免疫功能的影响及成分分析[J].中国医院药学杂志,2010,30(20):1740-1742.
[10]李英辉,张晶,孙莹,等.莪术提取液体内外抗肿瘤作用的实验研究[J].实用肿瘤医学杂志,2010,24(4):368-369.
[11]Malhotra A,Shanker A. NK cells:immune cross-talk and therapeutic implications[J]. Immunotherapy,2011,3(1):1143-1166.