不同种源杏香兔耳风绿原酸与芦丁含量变异分析
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摘要
为阐明杏香兔耳风绿原酸与芦丁含量的地理变异模式,确定其优良种源区。采用高效液相色谱法对全分布区9个地理杏香兔耳风种源绿原酸与芦丁含量特征进行了测定分析。结果表明不同种源杏香兔耳风绿原酸含量变异幅度为0.148～1.178 mg/g,芦丁变异幅度为0.227～0.537 mg/g,不同种源间绿原酸与芦丁含量差异分别达到极显著(P<0.01)和显著水平(P<0.05),种源间绿原酸与芦丁含量变异大于种源内,种源间表型分化系数分别为92.76%和95.73%;不同种源内植株间芦丁与绿原酸含量性状离散程度不同;绿原酸与芦丁含量与7月均温呈显著正相关,与其它地理生态因子相关性不显著;聚类分析可将9个种源划分为2个类群,低变异低绿原酸与芦丁含量类群和高变异高绿原酸含量类群。湖南洞口、浙江宁波、江西分宜和江西芦溪种源为高绿原酸与芦丁含量种源,是开展优良种质选择和发展林下种植的理想地区。
The study aims at comparing and analyzing the variation pattern of the chlorogenic acid and ru?tin content of Ainsliaea fragrans of different provenances and clarifing the superior provenances.METHODS The chlorogenic acid and rutin content of Ainsliaea fragrans from 9 nature distribution regions in cChina were mea?sured and analyzed.The results showed that there were extremely significant(P<0.01)and significant(P<0.05)differences among the chlorogenic acid and rutin contents from different regions.The chlorogenic acid and rutin content ranged from 0.148 mg/g to 1.178 mg/g and from 0.227 mg/g to 0.537 mg/g,respectively.The phenotypic differentiation coefficient(VST)of the chlorogenic acid and rutin content among provenances were 92.76% and95.73%,which indicated that the phenotypic variation mainly occurred within provenances.The chlorogenic ac?id and rutin content had a positive correlation with the mean temperature in July,and the relationship between the chlorogenic acid and rutin content of Ainsliaea fragrans and other geographical climate factors was not sig?nificant.According to the results of cluster analysis,nine Ainsliaea fragrans provenances could be divided into two groups namely,the provenance of lower variance and lower chlorogenic acid and rutin content and that of higher variance and higher chlorogenic acid and rutin content,in which the provenances from Dongkou of Hu?nan Province,Ningbo of Zhejiang Province and Fenyi of Jiangxi Province,and Luxi of Jiangxi Province were the superior provenances and suitable zones for the selection and cultivation of Ainsliaea fragrans under forests stand.
The study aims at comparing and analyzing the variation pattern of the chlorogenic acid and ru?tin content of Ainsliaea fragrans of different provenances and clarifing the superior provenances.METHODS The chlorogenic acid and rutin content of Ainsliaea fragrans from 9 nature distribution regions in cChina were mea?sured and analyzed.The results showed that there were extremely significant(P<0.01)and significant(P<0.05)differences among the chlorogenic acid and rutin contents from different regions.The chlorogenic acid and rutin content ranged from 0.148 mg/g to 1.178 mg/g and from 0.227 mg/g to 0.537 mg/g,respectively.The phenotypic differentiation coefficient(VST)of the chlorogenic acid and rutin content among provenances were 92.76% and95.73%,which indicated that the phenotypic variation mainly occurred within provenances.The chlorogenic ac?id and rutin content had a positive correlation with the mean temperature in July,and the relationship between the chlorogenic acid and rutin content of Ainsliaea fragrans and other geographical climate factors was not sig?nificant.According to the results of cluster analysis,nine Ainsliaea fragrans provenances could be divided into two groups namely,the provenance of lower variance and lower chlorogenic acid and rutin content and that of higher variance and higher chlorogenic acid and rutin content,in which the provenances from Dongkou of Hu?nan Province,Ningbo of Zhejiang Province and Fenyi of Jiangxi Province,and Luxi of Jiangxi Province were the superior provenances and suitable zones for the selection and cultivation of Ainsliaea fragrans under forests stand.
引文
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[5]邹盛勤,徐光辉,林惠,等.高效液相梯度洗脱法测定杏香兔耳风中绿原酸和芦丁[J].分析试验室,2008,27(5):105-109.Zou S Q,Xu G H,Lin H,et al.Determination of chlorogenic acid and rutin in Ainsliaea fragrans by RP-HPLC with gradientelution[J].Chinese Journal of Analysis Laboratory,2008,27(5):105-109.
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[7]Feng F,Chen M H,Xing C X,et al.Two novel sesquiterpenoids from Ainsliaea fragrans Champ[J].Journal of Asian naturalproducts research,2009(10):855-859.
[8]蒋俊毅,曹凯,吴德康,等.紫外分光光度法测定杏香兔耳风制剂中总黄酮含量[J].时珍国医国药,2005,16(3):178-179.Jiang J Y,Cao K,Wu D K,et al.Determination of the total flavonoids in Ainsliaea fragrans Champ by spectrophotometricmethod[J].Lishizhen Medicine and Materia Medica Research,2005,16(3):178-179.
[9]于宏,戴小英,江香梅.杏香兔耳风组培苗根系发育解剖学研究[J].江西林业科技,2008(6):25-27.Yu H,Dai X Y,Jiang X M.Anatomy on roots development for tissue cultural plants of Ainsliaea fragrans[J].Jiangxi ForestryScience and Technology,2008(6):25-27.
[10]黄骏,苏丹,宋永贵,等.不同产地杏香兔耳风酚酸类成分含量比较[J].中药材,2014,37(8):1340-1342.Huang J,Su D,Song Y G,et al.Quantitative analysis of phenolic acid in Ainsliaea fragrans from different habitats[J].Jour?nal of Chinese Medicinal Materials,2014,37(8):1340-1342.
[11]王小平,王九龄,刘晶岚,等.白皮松分布区的气候区划[J].林业科学,1999,35(4):102-107.Wang X P,Wang J L,Liu J L,et al.Climatic zoning of the distribution area of Pinus bungeana[J].Scientia Silvae Sinicae,1999,35(4):102-107.
[12]葛颂,王明庥,陈岳武.用同工酶研究马尾松群体的遗传结构[J].林业科学,1988,24(4):399-409.Ge S,Wang M X,Chen Y W.Study on genetic structure of Masson Pine population by isozyme[J].Scientia Silvae Sinicae,1988,24(4):399-409.
[13]Bajalan I,Pirbalouti A G. Variation in antibacterial activity and chemical compositions of essential oil from differentpopulations of myrtle[J].Industrial Crops&Products,2014,61:303-307.
[14]Djouahri A,Boualem S,Boudarene L,et al.Geographic′s variation impact on chemical composition,antioxidant and anti-inflammatory activities of essential oils from wood and leaves of Tetraclinis articulata(Vahl)Masters[J].Industrial Crops&Products,2015,63:138-146.
[15]Patel R P,Kumar R R,Singh R,et al.Study of genetic variability pattern and their possibility of exploitation in Ocimumgermplasm[J].Industrial Crops and Products,2015,66:119-122.
[2]国家中医药管理局.中华本草[M].上海:科学技术出版社,1999:668.State Administration of Traditional Chinese Medicine.Chinese herbal medicine[M].Shanghai:Science and TechnologyPress,1999:668.
[3]邢春秀,谢宁,杨念云,等.杏香兔耳风的化学成分[J].江苏药学与临床研究,2006,14(2):39-41.Xing C X,Xie N,Yang N Y,et al.Chemical constituents of Ainsliaea fragrans[J].Jiangsu Pharmacertical and Clinical Re?search,2006,14(02):39-41.
[4]刘戈,汪豪,吴婷,等.菊科植物杏香兔耳风的化学成分[J].中国天然药物,2007,5(4):266-268.Liu G,Wang H,Wu T,et al.Chemical constituents from Ainsliaea fragrans[J].Chinese Journal of Natural Medicine,2007,5(04):266-268.
[5]邹盛勤,徐光辉,林惠,等.高效液相梯度洗脱法测定杏香兔耳风中绿原酸和芦丁[J].分析试验室,2008,27(5):105-109.Zou S Q,Xu G H,Lin H,et al.Determination of chlorogenic acid and rutin in Ainsliaea fragrans by RP-HPLC with gradientelution[J].Chinese Journal of Analysis Laboratory,2008,27(5):105-109.
[6]江香梅,刘传林,胡小红,等.中药杏香兔耳风生物生态学特性及驯化栽培[J].江西林业科技,2006(4):10-12.Jiang X M,Liu C L,Hu X H,et al.Biological and ecological characteristics and cultivation of Ainsliaea fragrans[J].JiangxiForestry Science and Technology,2006(4):10-12.
[7]Feng F,Chen M H,Xing C X,et al.Two novel sesquiterpenoids from Ainsliaea fragrans Champ[J].Journal of Asian naturalproducts research,2009(10):855-859.
[8]蒋俊毅,曹凯,吴德康,等.紫外分光光度法测定杏香兔耳风制剂中总黄酮含量[J].时珍国医国药,2005,16(3):178-179.Jiang J Y,Cao K,Wu D K,et al.Determination of the total flavonoids in Ainsliaea fragrans Champ by spectrophotometricmethod[J].Lishizhen Medicine and Materia Medica Research,2005,16(3):178-179.
[9]于宏,戴小英,江香梅.杏香兔耳风组培苗根系发育解剖学研究[J].江西林业科技,2008(6):25-27.Yu H,Dai X Y,Jiang X M.Anatomy on roots development for tissue cultural plants of Ainsliaea fragrans[J].Jiangxi ForestryScience and Technology,2008(6):25-27.
[10]黄骏,苏丹,宋永贵,等.不同产地杏香兔耳风酚酸类成分含量比较[J].中药材,2014,37(8):1340-1342.Huang J,Su D,Song Y G,et al.Quantitative analysis of phenolic acid in Ainsliaea fragrans from different habitats[J].Jour?nal of Chinese Medicinal Materials,2014,37(8):1340-1342.
[11]王小平,王九龄,刘晶岚,等.白皮松分布区的气候区划[J].林业科学,1999,35(4):102-107.Wang X P,Wang J L,Liu J L,et al.Climatic zoning of the distribution area of Pinus bungeana[J].Scientia Silvae Sinicae,1999,35(4):102-107.
[12]葛颂,王明庥,陈岳武.用同工酶研究马尾松群体的遗传结构[J].林业科学,1988,24(4):399-409.Ge S,Wang M X,Chen Y W.Study on genetic structure of Masson Pine population by isozyme[J].Scientia Silvae Sinicae,1988,24(4):399-409.
[13]Bajalan I,Pirbalouti A G. Variation in antibacterial activity and chemical compositions of essential oil from differentpopulations of myrtle[J].Industrial Crops&Products,2014,61:303-307.
[14]Djouahri A,Boualem S,Boudarene L,et al.Geographic′s variation impact on chemical composition,antioxidant and anti-inflammatory activities of essential oils from wood and leaves of Tetraclinis articulata(Vahl)Masters[J].Industrial Crops&Products,2015,63:138-146.
[15]Patel R P,Kumar R R,Singh R,et al.Study of genetic variability pattern and their possibility of exploitation in Ocimumgermplasm[J].Industrial Crops and Products,2015,66:119-122.