硫酸化黑木耳多糖的辐射防护作用研究
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摘要
为考察硫酸化黑木耳多糖对~(60)Co-γ射线辐射损伤小鼠的防护作用。以硫酸化黑木耳多糖为受试物,用~(60)Co-γ射线对小鼠进行一次性全身辐射,对小鼠的免疫指标,血清中SOD活力、MDA含量,骨髓DNA含量和骨髓微核率进行测定。结果表明:硫酸化黑木耳多糖对~(60)Co-γ射线辐射损伤小鼠的单核细胞吞噬能力、血清SOD活性有明显的促进作用,并且能够增加小鼠免疫器官指数和骨髓DNA含量,减少血清MDA含量和骨髓微核率,减轻辐射诱导机体的氧化损伤。结论证实,硫酸化黑木耳多糖对~(60)Co-γ射线辐射损伤小鼠具有良好的防护作用。
In order to investigate the protective effect of sulfated Auricularia auricular polysaccharides on injured mice induced by ~(60)Co-γ. Sulfated Auricularia auricular polysaccharides were orally administered to groups of mice. After being exposed wholely to ~(60)Co-γ,immune parameters,SOD and MDA in serum,DNA and micronuclear rates in marrow were examined. Results showed that sulfated Auricularia auricular polysaccharides could increase the phagocytosis ability of monocyte and SOD activity in serum,decrease the formation of micronuclei in bone marrow and MDA in serum and reduce the oxidative damage of body induced by radiation. The conclusion of this experiment verified the fact that sulfated Auricularia auricular polysaccharides has protective effect on damaged male induced by ~(60)Co-γ.
In order to investigate the protective effect of sulfated Auricularia auricular polysaccharides on injured mice induced by ~(60)Co-γ. Sulfated Auricularia auricular polysaccharides were orally administered to groups of mice. After being exposed wholely to ~(60)Co-γ,immune parameters,SOD and MDA in serum,DNA and micronuclear rates in marrow were examined. Results showed that sulfated Auricularia auricular polysaccharides could increase the phagocytosis ability of monocyte and SOD activity in serum,decrease the formation of micronuclei in bone marrow and MDA in serum and reduce the oxidative damage of body induced by radiation. The conclusion of this experiment verified the fact that sulfated Auricularia auricular polysaccharides has protective effect on damaged male induced by ~(60)Co-γ.
引文
[1]贺许良,卢先州,陈枚,等.免疫器官辐射损伤与防护的研究进展[J].现代生物医学进展,2014(5):997-1000
[2]徐德鲲.黄芩苷对辐射损伤小鼠保护作用的研究[D].长春:吉林大学,2007
[3]Li X,Wang Z,Wang L.Polysaccharide of Hohenbuehelia serotina as a defense against damage by whole-body gamma irradiation of mice[J].Carbohydrate polymers,2013,94(2):829-835
[4]Tadayoni M,Sheikh-Zeinoddin M,Soleimanian-Zad S.Isolation of bioactive polysaccharide from acorn and evaluation of its functional properties[J].International journal of biological macromolecules,2015,72:179-184
[5]王宏芳,林晓晨,李雪静,等.松茸多糖对X射线照射小鼠造血功能的保护作用[J].吉林大学学报:医学版,2008,34(5):751-754
[6]Zhang L,Zhang W,Wang Q,et al.Purification,antioxidant and immunological activities of polysaccharides from Actinidia Chinensis roots[J].International journal of biological macromolecules,2015,72:975-983
[7]汪维云.灰树花多糖的抗辐射作用研究[J].安徽农业大学学报,2003,30(2):210-212
[8]Kulicke W M,Lettau A I,Thielking H.Correlation between immunological activity,molar mass,and molecular structure of different(1->3)-beta-D-glucans[J].Carbohydrate Research,1997,297(2):135-143
[9]于纯淼,于栋华,国立东,等.食用菌多糖抗辐射损伤作用研究进展[J].食用菌,2015,37(3):1-5
[10]Bhatia S,Rathee P,Sharma K,et al.Immuno-modulation effect of sulphated polysaccharide(porphyran)from Porphyra vietnamensis[J].International journal of biological macromolecules,2013,57:50-56
[11]Yokoya A,Shikazono N,Fujii K,et al.DNA damage induced by the direct effect of radiation[J].Radiation Physics and Chemistry,2008,77(10):1280-1285
[12]Reiter R J,Meltz M L,Herman T S.Melatonin:possible mechanisms involved in its‘radioprotective’effect[J].Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis,1998,404(1):187-189
[2]徐德鲲.黄芩苷对辐射损伤小鼠保护作用的研究[D].长春:吉林大学,2007
[3]Li X,Wang Z,Wang L.Polysaccharide of Hohenbuehelia serotina as a defense against damage by whole-body gamma irradiation of mice[J].Carbohydrate polymers,2013,94(2):829-835
[4]Tadayoni M,Sheikh-Zeinoddin M,Soleimanian-Zad S.Isolation of bioactive polysaccharide from acorn and evaluation of its functional properties[J].International journal of biological macromolecules,2015,72:179-184
[5]王宏芳,林晓晨,李雪静,等.松茸多糖对X射线照射小鼠造血功能的保护作用[J].吉林大学学报:医学版,2008,34(5):751-754
[6]Zhang L,Zhang W,Wang Q,et al.Purification,antioxidant and immunological activities of polysaccharides from Actinidia Chinensis roots[J].International journal of biological macromolecules,2015,72:975-983
[7]汪维云.灰树花多糖的抗辐射作用研究[J].安徽农业大学学报,2003,30(2):210-212
[8]Kulicke W M,Lettau A I,Thielking H.Correlation between immunological activity,molar mass,and molecular structure of different(1->3)-beta-D-glucans[J].Carbohydrate Research,1997,297(2):135-143
[9]于纯淼,于栋华,国立东,等.食用菌多糖抗辐射损伤作用研究进展[J].食用菌,2015,37(3):1-5
[10]Bhatia S,Rathee P,Sharma K,et al.Immuno-modulation effect of sulphated polysaccharide(porphyran)from Porphyra vietnamensis[J].International journal of biological macromolecules,2013,57:50-56
[11]Yokoya A,Shikazono N,Fujii K,et al.DNA damage induced by the direct effect of radiation[J].Radiation Physics and Chemistry,2008,77(10):1280-1285
[12]Reiter R J,Meltz M L,Herman T S.Melatonin:possible mechanisms involved in its‘radioprotective’effect[J].Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis,1998,404(1):187-189