黄芪总苷对微波辐射致大鼠精子损伤的治疗作用研究
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摘要
目的探讨黄芪总苷对微波辐射致大鼠精子损伤的治疗作用。方法 75只二级Wistar雄性大鼠,随机分为正常对照组、辐射对照组、40 mg/(kg·d)黄芪总苷组、80 mg/(kg·d)黄芪总苷和160 mg/(kg·d)黄芪总苷组,每组15只。采用30 mW/cm~2微波全身辐射大鼠15 min,于辐射后当天开始灌胃给药,各组给药量分别为40 mg/(kg·d)、80 mg/(kg·d)和160 mg/(kg·d),正常对照组和辐射对照组均给予等比体积的蒸馏水,每天1次,连续14 d。分别于给药7 d(辐射后7 d)、停药后6 h(辐射后14 d)、停药后7 d(辐射后21 d)处死大鼠,采用SCA精子动态分析系统检测大鼠精子活动度、活力参数、运动参数,HE染色观测精子畸形率。结果 30 mW/cm~2微波辐射后7 d、14 d、21 d,大鼠精子活动度、活力参数、运动参数下降,畸形率升高(P<0.05或P<0.01);给药7 d,停药后6 h、7 d,80~160 mg/(kg·d)黄芪总苷组与辐射对照组比较,大鼠精子活动度、活力参数、运动参数升高,畸形率下降(P<0.05或P<0.01)。结论 30 mW/cm~2微波辐射可引起大鼠精子损伤,表现为大鼠精子活动度、活力参数、运动参数下降,畸形率升高;80和160 mg/(kg·d)黄芪总苷具有明显治疗微波辐射致精子损伤的作用,有效剂量为80 mg/(kg·d)。
Objective To study the effect of astragaloside on sperm injury in rats induced by microwaveradiation. Methods Seventy five male Wistar rats were divided into 5 groups randomly: normal control(15 rats),microwave radiation control(15 rats),40 mg/(kg·d) astragaloside(15 rats),80 mg/(kg·d)astragaloside(15 rats) and 160 mg/(kg·d) astragaloside(15 rats) groups. The way of radiation was an average power density of 30 mW/cm~2 microwave radiation source with general uniform radiation 15 min.The rats received a daily intragastric administration from the day of radiation to 14 days after radiation once a day. The rats in each group were executed at 7 days of administration(7 days after radiation),6hours after drug withdrawal(14 days after radiation),7 days after drug withdrawal(21 days after radiation). The rat's sperm activate rate,motility parameters and motion parameters were detected by SCA sperm image analysis system. The malformation rate of sperms was calculated through H&E staining.Results The sperm activate rate,motility and motion parameters of rats were decreased(P < 0. 05 or P< 0. 01) and the sperm abnormality increased(P < 0. 05) at 7,14,21 day after 30 mW/cm~2 microwave radiation. The rat's sperm activate rate,motility and motion parameters were increased in 80 and 160 mg/(kg·d)astragaloside group compared to the radiation group. The ratio of sperm abnormality in 80 and 160mg/kg/d astragaloside groups decline D at 7 days of administration,6 hour and 7 days after the last intragastric administration(P < 0. 05 or P < 0. 01). Conclusions Microwave radiation at the average power densities of 30 mW/cm~2 could damage rats' sperms,such as the reduction of sperm motility,sperm motility parameters,sperm motion parameters and the increase of sperm abnormality; 80 ~ 160 mg/(kg · d)astragaloside groups exert therapeutic effect on injuries induced by microwave radiation. The effective dose of astragaloside on treating sperm injury induced by microwave radiation was 80 mg/(kg·d) astragaloside groups.
Objective To study the effect of astragaloside on sperm injury in rats induced by microwaveradiation. Methods Seventy five male Wistar rats were divided into 5 groups randomly: normal control(15 rats),microwave radiation control(15 rats),40 mg/(kg·d) astragaloside(15 rats),80 mg/(kg·d)astragaloside(15 rats) and 160 mg/(kg·d) astragaloside(15 rats) groups. The way of radiation was an average power density of 30 mW/cm~2 microwave radiation source with general uniform radiation 15 min.The rats received a daily intragastric administration from the day of radiation to 14 days after radiation once a day. The rats in each group were executed at 7 days of administration(7 days after radiation),6hours after drug withdrawal(14 days after radiation),7 days after drug withdrawal(21 days after radiation). The rat's sperm activate rate,motility parameters and motion parameters were detected by SCA sperm image analysis system. The malformation rate of sperms was calculated through H&E staining.Results The sperm activate rate,motility and motion parameters of rats were decreased(P < 0. 05 or P< 0. 01) and the sperm abnormality increased(P < 0. 05) at 7,14,21 day after 30 mW/cm~2 microwave radiation. The rat's sperm activate rate,motility and motion parameters were increased in 80 and 160 mg/(kg·d)astragaloside group compared to the radiation group. The ratio of sperm abnormality in 80 and 160mg/kg/d astragaloside groups decline D at 7 days of administration,6 hour and 7 days after the last intragastric administration(P < 0. 05 or P < 0. 01). Conclusions Microwave radiation at the average power densities of 30 mW/cm~2 could damage rats' sperms,such as the reduction of sperm motility,sperm motility parameters,sperm motion parameters and the increase of sperm abnormality; 80 ~ 160 mg/(kg · d)astragaloside groups exert therapeutic effect on injuries induced by microwave radiation. The effective dose of astragaloside on treating sperm injury induced by microwave radiation was 80 mg/(kg·d) astragaloside groups.
引文
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[13]李健,韩林,马玉芳,等.黄芪3种成分对Chang Liver细胞氧化应激的抑制作用[J].中国中药杂志,2015,40(2):318-323.Li Jian,Han Lin,Ma Yufang,et al.Inhibiting effects of three components of Astragalus membranaceus on oxidative stress in Chang Liver cells[J].China Journal of Chinese Materia Medica,2015,40(2):318-323.
[2]Adel Z,Ayman Z,Dalia S,et al.In vitro effect of cell phone radiation on motility,DNA fragmentation and clusterin gene expression in human sperm[J].Int J Fertil Steril,2015,9(1):129-136.
[3]索永善,叶玲玲,马川花,等.雷达微波辐射对精子形态的影响[J].中华男科学杂志,2007,13(12):1132-1135.Suo Yongshan,Ye Lingling,Ma Chanhua et al.Radar microwave radiation on the infiuence of sperm morphology[J].National Journal of Andrology,2007,13(12):1132-1135.
[4]李静,张静,彭瑞云,等.安多霖对微波辐射致大鼠免疫损伤的保护作用[J].中国公共卫生,2011,27(10):1269-1270.Li Jing,Zhang Jing,Peng Ruiyun,et al.Protective effect of aduola fuzhenglin on immune injury induced by microwave exposure in rats[J].Chin J Public Health,2011,27(10):1269-1270.
[5]郝述霞,王春燕,齐雪松,等.安多霖对微波辐照大鼠生殖及神经系统的防护作用[J].辐射防护,2011,31(5):313-316.Hao Shuxia,Wang Chunyan,Qi Xuesong,et al.Impact of Anduolin on rat sex hormones and redox system exposed to high power microwave radiation[J].Radiation Protection,2011,31(5):313-316.
[6]王凤娟,王伟,李蓉,等.巴戟天根二种提取物对微波辐射大鼠生精功能的影响[J].中华男科学杂志,2013,19(4):340-345.Wang Fengjuan,Wang Wei,Li Rong,et al.Morinda Officialis how extract improves microwave-induced reproductive impairment in male rats[J].National Journal of Andrology,2013,19(4):340-345.
[7]张燕,郭贤坤,刘汉丹,等.黄芪注射液体外对精子运动相关参数的影响[J].中药材,2010,33(3):485-486.Zhang Yan,Guo Xiankun,Liu Handan,et al.Effects of Huangqi injection on sperm motility and sperm activity in vitro[J].Journal of Chinese Medicinal Material,2010,33(3):485-486.
[8]梁培育,李浩勇,叶章群,等.添加黄芪注射液对人精子线粒体功能的影响[J].中华男科学,2004,18(3):11-13.Liang Peiyu,Li Haoyong,Ye Zhangqun,et al.Effect of Astragalus membranaceus injection on human sperm mitochondrial activity in vitro[J].National Journal of Andrology,2004,18(3):11-13.
[9]Ma X,Zhang K,Li H,et al.Extracts from Astragalus membranaceus limit myocardial cell death and improve cardiac function in a rat model of myocardial ischemia[J].J Ethnopharmacol,2013,149(3):720-728.
[10]Kumar S,Kesari K K,Behari J,et al.Impact of microwave at X-band in the aetiology of male infertility[J].Electromagn Biol Med,2012,31(3):223-232.
[11]穆慧玲,张清俊,杨昌林,等.高功率微波辐射对小鼠睾丸氧化应激及ATPase的影响[J].职业与健康,2013,29(9):1091-1093.Mu Huiling,Zhang Qingjun,Yang Changlin,et al.Effect of high power microwave radiation on oxidative stress and ATPase activity of testis in mice[J].Occup and Health,2013,29(9):1091-1093.
[12]Cao Y,Ruan Y,Shen T,et al.Astragalus polysaccharide suppresses doxorubicin-induced cardiotoxicity by regulating the PI3k/Akt and p38MAPK pathways[J].Oxid Med Cell Longev,2014,16(10):1151-1160.
[13]李健,韩林,马玉芳,等.黄芪3种成分对Chang Liver细胞氧化应激的抑制作用[J].中国中药杂志,2015,40(2):318-323.Li Jian,Han Lin,Ma Yufang,et al.Inhibiting effects of three components of Astragalus membranaceus on oxidative stress in Chang Liver cells[J].China Journal of Chinese Materia Medica,2015,40(2):318-323.