吡咯喹啉醌在抗腮腺辐射损伤的保护作用研究
|
摘要
目的:研究吡咯喹啉醌(PQQ)通过对全身辐射(TBI)诱导的C57BL/6J小鼠腮腺损伤的保护作用。方法:随机将30只8周龄雌性C57BL/6J小鼠分成3组(n=10):未治疗组;4 Gy TBI组;4 Gy TBI加正常饮食添加PQQ组。4周后,取腮腺组织进行病理学和生化指标的测定。结果:PQQ部分纠正了TBI诱导的腮腺损伤,主要通过多个方面发挥对受损腮腺的辐射保护作用,如促细胞增殖、抑制细胞凋亡和衰老、上调抗氧化能力、清除氧自由基、减少DNA损伤。结论:PQQ可能通过上调抗氧化能力、抑制氧化应激,参与DNA损伤修复作用,从而发挥对TBI诱导腮腺损伤的辐射保护作用。
Objective: To investigate the effects of pyrroloquinoline quinone( PQQ) on paratid gland damage induced by total body irradiation( TBI). Methods: 30 female 8-week-old C57 BL/6 J mice were randomly assigned into 3 groups( n = 10) : Untreated; 4 gray( Gy) X-ray radiation and 4 Gy X-ray radiation with additional dietary of PQQ. After 4 weeks,parotid glands tissues were collected for evaluating pathological and biochemical parameters. Results: The results indicated that PQQ partially rescued TBI induced damage of parotid glands. PQQ played radio-protective effects on parotid glands through multiple aspects,such as promoting cell proliferation,inhibiting cell apoptosis and senescence,up-regulating antioxidant ability,scavenging ROS and reducing DNA damage.Conclussion: PQQ plays a radio-protective role in parotid gland damage induced by TBI,possibly via inhibiting oxidative stress and participating in DNA damage repair.
Objective: To investigate the effects of pyrroloquinoline quinone( PQQ) on paratid gland damage induced by total body irradiation( TBI). Methods: 30 female 8-week-old C57 BL/6 J mice were randomly assigned into 3 groups( n = 10) : Untreated; 4 gray( Gy) X-ray radiation and 4 Gy X-ray radiation with additional dietary of PQQ. After 4 weeks,parotid glands tissues were collected for evaluating pathological and biochemical parameters. Results: The results indicated that PQQ partially rescued TBI induced damage of parotid glands. PQQ played radio-protective effects on parotid glands through multiple aspects,such as promoting cell proliferation,inhibiting cell apoptosis and senescence,up-regulating antioxidant ability,scavenging ROS and reducing DNA damage.Conclussion: PQQ plays a radio-protective role in parotid gland damage induced by TBI,possibly via inhibiting oxidative stress and participating in DNA damage repair.
引文
[1]Logemann JA,Pauloski BR,Rademaker AW,et al.Xerostomia:12-month changes in saliva production and its relationship to perception and performance of swallow function,oral intake,and diet after chemoradiation[J].Head Neck,2003,25(6):432-437.
[2]Wang Y,Liu L,Pazhanisamy SK,et al.Total body irradiation causes residual bone marrow injury by induction of persistent oxidative stress in murine hematopoietic stem cells[J].Free Radic Biol Med,2010,48(2):348-356.
[3]Duine JA.Cofactor diversity in biological oxidations:Implications and applications[J].Chem Rec,2001,1(1):74-83.
[4]Mitchell AE,Jones AD,Mercer RS,et al.Characterization of pyrroloquinoline quinone amino acid derivatives by electrospray ionization mass spectrometry and detection in human milk[J].Anal Biochem,1999,269(2):317-325.
[5]Kumazawa T,Sato K,Seno H,et al.Levels of pyrroloquinoline quinone in various foods[J].Biochem J,1995,307(Pt 2):331-333.
[6]黄元清,陈宁,苗登顺.吡咯并喹啉醌对Bmi-1基因敲除小鼠下颌骨及牙发育障碍的治疗作用及机制研究[J].中华口腔医学杂志,2015,50(8):496-502.
[7]Dirix P,Nuyts S,Van den Bogaert W.Radiation-induced xerostomia in patients with head and neck cancer:A literature review[J].Cancer,2006,107(11):2525-2534.
[8]Umegaki K,Ichikawa T.Decrease in vitamin E levels in the bone marrow of mice receiving whole-body X-ray irradiation[J].Free Radic Biol Med,1994,17(5):439-444.
[9]Pontual ML,Tuji FM,Barros SP,et al.Ultrastructural evaluation of the radioprotective effect of sodium selenite on submandibular glands in rats[J].J Appl Oral Sci,2007,15(3):162-168.
[10]Medina VA,Prestifilippo JP,Croci M,et al.Histamine prevents functional and morphological alterations of submandibular glands induced by ionising radiation[J].Int J Radiat Biol,2011,87(3):284-292.
[11]卢浩,张赐童,刘士维,等.腺泡细胞在大鼠腮腺组织萎缩过程中的变化规律[J].实用口腔医学杂志,2015,5(31):623-626.
[12]李小娜,范芹,黄伟琨,等.茶多酚对脂多糖介导下人牙周膜成纤维细胞MMP-1、MMP-2表达的影响[J].实用口腔医学杂志,2014,30(6):774-777.
[2]Wang Y,Liu L,Pazhanisamy SK,et al.Total body irradiation causes residual bone marrow injury by induction of persistent oxidative stress in murine hematopoietic stem cells[J].Free Radic Biol Med,2010,48(2):348-356.
[3]Duine JA.Cofactor diversity in biological oxidations:Implications and applications[J].Chem Rec,2001,1(1):74-83.
[4]Mitchell AE,Jones AD,Mercer RS,et al.Characterization of pyrroloquinoline quinone amino acid derivatives by electrospray ionization mass spectrometry and detection in human milk[J].Anal Biochem,1999,269(2):317-325.
[5]Kumazawa T,Sato K,Seno H,et al.Levels of pyrroloquinoline quinone in various foods[J].Biochem J,1995,307(Pt 2):331-333.
[6]黄元清,陈宁,苗登顺.吡咯并喹啉醌对Bmi-1基因敲除小鼠下颌骨及牙发育障碍的治疗作用及机制研究[J].中华口腔医学杂志,2015,50(8):496-502.
[7]Dirix P,Nuyts S,Van den Bogaert W.Radiation-induced xerostomia in patients with head and neck cancer:A literature review[J].Cancer,2006,107(11):2525-2534.
[8]Umegaki K,Ichikawa T.Decrease in vitamin E levels in the bone marrow of mice receiving whole-body X-ray irradiation[J].Free Radic Biol Med,1994,17(5):439-444.
[9]Pontual ML,Tuji FM,Barros SP,et al.Ultrastructural evaluation of the radioprotective effect of sodium selenite on submandibular glands in rats[J].J Appl Oral Sci,2007,15(3):162-168.
[10]Medina VA,Prestifilippo JP,Croci M,et al.Histamine prevents functional and morphological alterations of submandibular glands induced by ionising radiation[J].Int J Radiat Biol,2011,87(3):284-292.
[11]卢浩,张赐童,刘士维,等.腺泡细胞在大鼠腮腺组织萎缩过程中的变化规律[J].实用口腔医学杂志,2015,5(31):623-626.
[12]李小娜,范芹,黄伟琨,等.茶多酚对脂多糖介导下人牙周膜成纤维细胞MMP-1、MMP-2表达的影响[J].实用口腔医学杂志,2014,30(6):774-777.