14个八仙花品种亲缘关系的ISSR分析
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摘要
利用ISSR技术对14个八仙花品种进行了亲缘关系的研究。从49个引物(引物选自加拿大哥伦比亚大学UBC公司公布的100条引物)中筛选了8个条带清晰、多态性高、重复性好的引物,对14个八仙花品种DNA进行扩增,共扩增出478条带(平均每个引物扩增59.75条DNA片段),多态性条带420条。用POPGENE分析数据得到14个品种的遗传距离为0.000 2～0.087 4,遗传相似性系数为0.916 3～1.000 0;利用NTSYSpc 2.1聚类分析,在遗传相似性系数约为0.38处将14个八仙花品种明显分为两大类,其中花园蕾丝、绿色夏天和圣代草莓被聚为第一类,其余11个品种被聚为第二类。
Genetic relationship of 14 cultivars of Hydrangea was assessed using inter-simple sequence repeat marker.Eight primers were screened out from 49 random primers,a total of 478 DNA fragments were amplified and 420 DNA fragments were polymorphism bands.As analyzed by POPGENE,the genetic distance among populations was with 0.000 2-0.087 4and genetic similarity coefficient was 0.916 3-1.000 0;UPGMA cluster analysis in the genetic similarity coefficient of 0.38,14 Hydrangea cultivars were divided into two groups,one group included H.‘Huayuanleisi',H.‘Lvsexiatian'and H.‘Shengdaicaomei',and the other Hydrangeacultivars were in another group.
Genetic relationship of 14 cultivars of Hydrangea was assessed using inter-simple sequence repeat marker.Eight primers were screened out from 49 random primers,a total of 478 DNA fragments were amplified and 420 DNA fragments were polymorphism bands.As analyzed by POPGENE,the genetic distance among populations was with 0.000 2-0.087 4and genetic similarity coefficient was 0.916 3-1.000 0;UPGMA cluster analysis in the genetic similarity coefficient of 0.38,14 Hydrangea cultivars were divided into two groups,one group included H.‘Huayuanleisi',H.‘Lvsexiatian'and H.‘Shengdaicaomei',and the other Hydrangeacultivars were in another group.
引文
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[23]黄丽莉.八仙花周年供应与繁育技术研究[A].中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心.中国观赏园艺研究进展2008——中国园艺学会观赏园艺专业委员会2008年学术年会论文集[C].中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心,2008:4.
[24]陈亚军,于莹,高岩,等.八仙花叶中酚酸类化合物的分离和结构鉴定[J].中医药信息,2017(1):10-13.
[25]卫兆芬.中国绣球属植物的修订[J].广西植物,1994,14(2):101-121.
[26]李艳香,李达,李炎林,等.八仙花SRAP反应体系的建立与优化[J].湖南农业科学,2008(6):14-16,45.
[27]李艳香.19个绣球属种质资源的SRAP分析[D].湖南农业大学,2009.
[28]刘子金,陈伟岸,袁名杰,等.岭南山竹子种质资源遗传多样性及亲缘关系的ISSR分析[J].分子植物育种,2016(4):966-972.
[29]林立,王志龙,付涛,等.39个樱花品种亲缘关系的ISSR分析[J].植物研究,2016(2):297-304.
[30]徐静静,张良英,赵冰,等.色季拉山8个杜鹃花野生种亲缘关系的ISSR分析[J].种子,2016,35(3):1-4.
[31]李晓玲,杨进,张雄,等.10份续断属植物亲缘关系的ISSR分析(英文)[J].植物研究,2015(3):450-456.
[32]肖黎,李晓玲,王玉兵,等.22种木莲属植物亲缘关系的ISSR分析[J].植物研究,2011(4):489-494.
[33]Maximowicz,C.J.Revision Hydrangesrum ASIAE Orientalis[J].Mem.Acad.Sci.Petersb.Ser.,1867,7,10(16):6-18.
[34]Rehder,A.Saxifragaceae-Hydrangea.In:Sargent(ed.),Pl.Wills,1911-1913,l:25-41,150-151,579.
[35]Enger,A.Hydrangea in Enger and Plant,Nat.Pfanzenfam[J].Auf,1930,2,18a:201-207.
[36]陈焕镛.绣球亚科资料的研究[J].植物分类学报,1954,3(2):108-161.
[37]MeClintoek,E.Thecultivated Hydrangeas[J].Baileya,1956,4:165-175.
[38]Hu Z Y,Lin L,Deng J F,et al.Genetic diversity and differentiation among populations of Bretschneidera sinensis,(Bretschneideraceae),a narrowly distributed and endemic species in China,detected by inter-simple sequence repeat(ISSR)[J].Biochemical Systematics&Ecology,2014,56(56):104-110.
[39]林立,胡仲义,王国明.舟山群岛野生山茶种群遗传多样性的ISSR分析[J].植物遗传资源学报,2013,14(4):594-599.
[40]吴大鹏,房嫌嫌,崔闰根.国内外陆地棉品种资源的亲缘关系和遗传多态性研究[J].棉花学报,2011,23(4):291-299.
[2]蔡能,杨玉勇.绣球花品种介绍[J].中国花卉园艺,2009(22):20-22.
[3]赵冰.中国八仙花[M].北京:中国林业出版社,2016,4.
[4]罗正风,吴逍.八仙花商品盆花生产技术[J].南方农业,2014,8(16):17-18.
[5]种高军,石胜荣.绣球精品化盆栽技术[J].中国花卉园艺,2015(14):25.
[6]方亭.八仙花花切花保鲜技术[N].中国花卉报,2013-07-01.
[7]昆明杨月季园艺.绣球花采后处理及花店养护[N].中国花卉报,2018-08-28.
[8]Dirr,Michael A.Dirr's Encyclopedia of Trees&shrubs[M].Timber Press,2011.
[9]张艳双,刁春武,陈奎礼,等.八仙花专类品种园景观规划设计探析[J].安徽农业科学,2014,42(26):9 036-9 037.
[10]蔡向阳.夏季花园主角-绣球[J].中国花卉园艺,2013(12):24-25.
[11]王知斌,梁军,夏永刚,等.八仙花叶的化学成分及药理作用研究进展[J].中医药信息,2012,29(5):114-115.
[12]C.J.van Gelderen,D.M.van Gelderen Encyclopedia of Hydrangeas[M].Timber Press,2004.
[13]Zhang H,Hisashi M,Akfia K,et al.New type of anti-diabetic compounds from the processed leaves of Hydrangeae macrophylla seringe var.thunbergli[J].Bioorganic&Medicinal Chemistry Letters,2007,17:4 972-4 976.
[14]Ryusuke N,Erika H,Shun K,et al.Suppresion by Hydrangeae dulcis folium of D-galactosamine-inducedliver injury in vitro and in vivo[J].Bioscience Bioteehnology&Biochemistry,2003,67(12):2 641-2 643.
[15]MeClintock,E.amonograph of the genus Hyarangea[J].Proc.Calif.Aead.Sei,1957,29:147-256.
[16]戚继忠,由士江,王洪俊,等.园林植物清除细菌能力的研究[J],城市环境与城市生态,2000,13(4):36-38.
[17]孔翔.圆锥绣球(Hydrangea paniculata)对铅胁迫的生理响应及铅在其体内的积累特性[D]江西:江西财经大学园林植物与观赏园艺,2009.
[18]陈烈华,王晓荷,种楠,等.绣球属种质资源及其开发利用[J].现代农业科技,2011(14):250-251.
[19]焦隽,梁丽君,马辉,等.绣球种质资源引进与栽培技术[J].现代农业科技,2016(11):203-204.
[20]李宪章.适合北方陆地种植的八仙花[J].中国花卉盆景,2006(12):8.
[21]张黎,王培.不同因子对盆栽八仙花扦插生根的影响[J].北方园艺,2012(11):73-76.
[22]李晓晨.八仙花栽培技术[J].现代农业科技,2007(9):49,54.
[23]黄丽莉.八仙花周年供应与繁育技术研究[A].中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心.中国观赏园艺研究进展2008——中国园艺学会观赏园艺专业委员会2008年学术年会论文集[C].中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心,2008:4.
[24]陈亚军,于莹,高岩,等.八仙花叶中酚酸类化合物的分离和结构鉴定[J].中医药信息,2017(1):10-13.
[25]卫兆芬.中国绣球属植物的修订[J].广西植物,1994,14(2):101-121.
[26]李艳香,李达,李炎林,等.八仙花SRAP反应体系的建立与优化[J].湖南农业科学,2008(6):14-16,45.
[27]李艳香.19个绣球属种质资源的SRAP分析[D].湖南农业大学,2009.
[28]刘子金,陈伟岸,袁名杰,等.岭南山竹子种质资源遗传多样性及亲缘关系的ISSR分析[J].分子植物育种,2016(4):966-972.
[29]林立,王志龙,付涛,等.39个樱花品种亲缘关系的ISSR分析[J].植物研究,2016(2):297-304.
[30]徐静静,张良英,赵冰,等.色季拉山8个杜鹃花野生种亲缘关系的ISSR分析[J].种子,2016,35(3):1-4.
[31]李晓玲,杨进,张雄,等.10份续断属植物亲缘关系的ISSR分析(英文)[J].植物研究,2015(3):450-456.
[32]肖黎,李晓玲,王玉兵,等.22种木莲属植物亲缘关系的ISSR分析[J].植物研究,2011(4):489-494.
[33]Maximowicz,C.J.Revision Hydrangesrum ASIAE Orientalis[J].Mem.Acad.Sci.Petersb.Ser.,1867,7,10(16):6-18.
[34]Rehder,A.Saxifragaceae-Hydrangea.In:Sargent(ed.),Pl.Wills,1911-1913,l:25-41,150-151,579.
[35]Enger,A.Hydrangea in Enger and Plant,Nat.Pfanzenfam[J].Auf,1930,2,18a:201-207.
[36]陈焕镛.绣球亚科资料的研究[J].植物分类学报,1954,3(2):108-161.
[37]MeClintoek,E.Thecultivated Hydrangeas[J].Baileya,1956,4:165-175.
[38]Hu Z Y,Lin L,Deng J F,et al.Genetic diversity and differentiation among populations of Bretschneidera sinensis,(Bretschneideraceae),a narrowly distributed and endemic species in China,detected by inter-simple sequence repeat(ISSR)[J].Biochemical Systematics&Ecology,2014,56(56):104-110.
[39]林立,胡仲义,王国明.舟山群岛野生山茶种群遗传多样性的ISSR分析[J].植物遗传资源学报,2013,14(4):594-599.
[40]吴大鹏,房嫌嫌,崔闰根.国内外陆地棉品种资源的亲缘关系和遗传多态性研究[J].棉花学报,2011,23(4):291-299.