医学研究生分子细胞生物学技术实验课的探索与改革
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摘要
分子细胞生物学是在分子水平研究细胞生命活动基本规律的前沿学科。在研究生教学中开设分子细胞生物学技术实验课,可以有效地提高研究生科研思维及动手能力。为此,中国医科大学分子细胞生物学教研室在研究生选修课教学中设置了分子细胞生物学技术实验课。通过系统理论讲授与实验操作相结合的方式,不仅启迪了研究生(特别是临床型研究生)的科研思路,同时通过研究生亲自参与分子克隆、细胞培养、荧光素酶双报告基因检测等实验操作及数据分析,提高了研究生的动手操作和科研分析能力,为研究生未来的科学研究奠定了基础。
Molecular cell biology is a frontier subject of life sciences,and itstudies the basic rules of cell life activities at the molecular level. The experimental course of molecular cell biology technology in postgraduate teaching is of great significance to the improvement of postgraduate research thinking and practical ability. To this end,the Department of Molecular Cell Biology of China Medical University set up an experimental course on molecular cell biology technology in the teaching of postgraduate selective courses. Through the combination of systematic theoretical teaching and experimental operation,it enlightened the scientific research ideas of postgraduates( especially clinical postgraduates). Through participation in experimental operations and data analysis,such as molecular cloning,cell culture,andluciferase double reporter gene detection,the postgraduate students improved their hands-on operation and scientific analysis capabilities and laid a foundation for scientific research of postgraduates.
Molecular cell biology is a frontier subject of life sciences,and itstudies the basic rules of cell life activities at the molecular level. The experimental course of molecular cell biology technology in postgraduate teaching is of great significance to the improvement of postgraduate research thinking and practical ability. To this end,the Department of Molecular Cell Biology of China Medical University set up an experimental course on molecular cell biology technology in the teaching of postgraduate selective courses. Through the combination of systematic theoretical teaching and experimental operation,it enlightened the scientific research ideas of postgraduates( especially clinical postgraduates). Through participation in experimental operations and data analysis,such as molecular cloning,cell culture,andluciferase double reporter gene detection,the postgraduate students improved their hands-on operation and scientific analysis capabilities and laid a foundation for scientific research of postgraduates.
引文
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[9]廖丹,徐云飞,孟莎莎,等.48孔板荧光素酶报告基因实验边缘效应[J].研究报告,2018,37(9):3792-3798.
[10]MUHAMMAD J.The Crispr/Cas system:emerging technology and application[M].Pittsburgh:Caister Academic Press,2017:1-14.
[11]KNOTT GJ,DOUDNA JA.CRISPR-Cas guides the future of genetic engineering[J].Science,2018,361(6405):866-869.
[12]WANG HX,LI M,LEE CM,et al.CRISPR/Cas9-based genome editing for disease modeling and therapy:challenges and opportunities for nonviral delivery[J].Chem Rev,2017,117(15):9874-9906.
[2]吕建新,王晓春.临床分子生物学检验技术[M].北京:人民卫生出版社,2016:1-278.
[3]韩燕,蒋丛姗,孙健.实验主导的医学研究生细胞分子生物学教学改革[J].西北医学教育,2015,23(6):992-995.
[4]杨晓玲,姜怡邓,田珏,等.基础医学实验技术课程体系的构建与实践[J].基础医学教育,2017,19(12):959-961.
[5]刘岩岩.医学分子生物学教学模式改革的初步探讨[J].科教文汇,2017,398:57-60.
[6](美)M.R.格林,J.萨姆布鲁克.分子克隆实验指南[M].4版.贺福初,译.北京:科学出版社,2017:1474-1481.
[7]SMIRNOVA DV,UGAROVA NN.Firefly luciferase-based fusion proteins and their applications in bioanalysis[J].Photochem Photobiol,2017,93(2):436-447.
[8]薛秀花,黄晨洗.荧光素酶基因的研究进展[J].生物学通报,2013,48(9):1-4.
[9]廖丹,徐云飞,孟莎莎,等.48孔板荧光素酶报告基因实验边缘效应[J].研究报告,2018,37(9):3792-3798.
[10]MUHAMMAD J.The Crispr/Cas system:emerging technology and application[M].Pittsburgh:Caister Academic Press,2017:1-14.
[11]KNOTT GJ,DOUDNA JA.CRISPR-Cas guides the future of genetic engineering[J].Science,2018,361(6405):866-869.
[12]WANG HX,LI M,LEE CM,et al.CRISPR/Cas9-based genome editing for disease modeling and therapy:challenges and opportunities for nonviral delivery[J].Chem Rev,2017,117(15):9874-9906.