牡蛎低聚肽配伍核桃低聚肽和山药多糖对雄性小鼠性功能的影响
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摘要
目的探讨牡蛎低聚肽配伍核桃低聚肽和山药多糖对雄性小鼠性功能的影响。方法将50只健康清洁级成年雄性ICR小鼠随机分为空白对照组、乳清蛋白组(760 mg/kg bw)和3个牡蛎低聚肽配伍核桃低聚肽和山药多糖配伍组(配伍1组:160+440+220 mg/kg bw;配伍2组:320+220+220 mg/kg bw;配伍3组:640+110+220 mg/kg bw)。连续灌胃干预45 d,在干预的第15 d,30 d和45 d分别对小鼠进行交配实验记录骑跨潜伏期、20 min内骑跨次数及交配次数;干预结束后,检测小鼠血清NO和睾酮浓度,海绵体NO、c GMP和PDE5水平,分别取睾丸、附睾、包皮腺、精囊和前列腺并精确称重。结果与乳清蛋白组相比,3个配伍组可不同程度的增加雄性小鼠附性器官指数,提高血清NO和睾酮含量,提高海绵体c GMP含量并降低PDE5水平,此外还可缩短小鼠骑跨潜伏期并增加骑跨次数和交配次数。结论牡蛎低聚肽配伍核桃低聚肽和山药多糖能够增强雄性小鼠的性功能。
Objective To investigate the effect of compatibilities of oyster oligopeptides, walnut oligopeptides and yam polysaccharide on sexual function in male mice. Methods A total of 50 adult ICR male mice were randomized into five groups, including naive control group, whey protein group(760 mg/kg bw), and 3 composite groups of oyster oligopeptides,walnut oligopeptides and yam polysaccharide(composite group 1, 160 + 440 + 220 mg/kg bw; composite group 2: 320 + 220+ 220 mg/kg bw; composite group 3, 640 + 110 + 220 mg/kg bw). Test substances were gavaged for 45 days. The sexual activity of male mice, such as incubation period of the first straddle and total number of intercourse and straddle in 20 min were recorded on days 15, 30, 45. After the intervention, the concentration of serum NO and testosterone in mice were detected. The level of NO, c GMP and PDE5 in corpus cavernosum tissue were measured. Testicles, epididymis, preputial glands, seminal vesicles and prostate glands of male mice were all weighed. Results Compared with the whey protein group,3 composite groups could increase the weight of accessory sex organs and the concentration of serum NO and testosterone,increase the level of c GMP and decrease PDE5 in corpus cavernosum tissue to some extent. What 's more, the mount latency was decreased and the total number of mount and intercourse were both increased. Conclusion Compatibilities of oyster oligopeptides, walnut oligopeptides and yam polysaccharide could improve the sexual function in male mice.
Objective To investigate the effect of compatibilities of oyster oligopeptides, walnut oligopeptides and yam polysaccharide on sexual function in male mice. Methods A total of 50 adult ICR male mice were randomized into five groups, including naive control group, whey protein group(760 mg/kg bw), and 3 composite groups of oyster oligopeptides,walnut oligopeptides and yam polysaccharide(composite group 1, 160 + 440 + 220 mg/kg bw; composite group 2: 320 + 220+ 220 mg/kg bw; composite group 3, 640 + 110 + 220 mg/kg bw). Test substances were gavaged for 45 days. The sexual activity of male mice, such as incubation period of the first straddle and total number of intercourse and straddle in 20 min were recorded on days 15, 30, 45. After the intervention, the concentration of serum NO and testosterone in mice were detected. The level of NO, c GMP and PDE5 in corpus cavernosum tissue were measured. Testicles, epididymis, preputial glands, seminal vesicles and prostate glands of male mice were all weighed. Results Compared with the whey protein group,3 composite groups could increase the weight of accessory sex organs and the concentration of serum NO and testosterone,increase the level of c GMP and decrease PDE5 in corpus cavernosum tissue to some extent. What 's more, the mount latency was decreased and the total number of mount and intercourse were both increased. Conclusion Compatibilities of oyster oligopeptides, walnut oligopeptides and yam polysaccharide could improve the sexual function in male mice.
引文
[1]凌小林.男性性功能障碍的国内研究现状[J].中国现代医生,2015,53(11):157-160.
[2]李维,兰海楠,郭风,等.生物活性肽的研究进展[J].中国畜牧兽医,2012,39(10):105-107.
[3]陈彩霞,苏秀兰,韩瑞兰.生物活性肽调节免疫功能的研究现状[J].现代预防医学,2016,43(15):2798-2801.
[4]杨韵,徐波.牡蛎的化学成分及其生物活性研究进展[J].中国现代中药,2015,12(12):1345-1349.
[5]江长优,张健,赵江贺.牡蛎多糖增强小鼠免疫功能作用研究[J].中成药,2013,35(5):1062-1065.
[6]吉宏武,苗建银,邵海艳,等.近江牡蛎肉水解物的营养成分及抗疲劳作用研究[J].食品科技,2010,35(2):70-73.
[7]李江滨,侯敢,赖银璇.牡蛎多糖抑制流感病毒增殖的实验研究[J].时珍国医国药,2009,20(6):1346-1347.
[8]秦小明,林华娟,张自然,等.牡蛎肉的醒酒作用机理初探[J].渔业科学进展,2011,32(1):109-113.
[9]陈悦,李路,闫朝阳,等.小分子牡蛎多肽对雄性小鼠性功能的影响[J/OL].(2017-09-04)[2018-05-07).http://kns.cnki.net/kcms/detail/45.1369.Q.20170906.0733.002.html.
[10]杨子明,刘金磊,颜小捷,等.核桃多肽对D-半乳糖诱导老年小鼠血脂水平的影响[J].食品科学,2015,36(9):181-184.
[11]侯雅坤.核桃活性肽体外抗氧化活性及ACE抑制活性的研究[D].北京:北京林业大学,2012.
[12]裴晓惠,张佳程,齐建勋,等.核桃蛋白肽脱苦及其对ACE抑制活性的影响[J].食品工业科技,2012,33(20):236-239.
[13]贾靖霖,蒲云峰,李虎,等.核桃多肽抗疲劳作用的研究[J].食品工业科技,2014,35(7):340-342.
[14]Robbins WA,Xun L,Fitzgerald LZ,et al.Walnuts improve semen quality in men consuming a Western-Style Diet:randomized control dietary intervention trial[J].Biology of Reproduction,2012,87(4):101.
[15]赵贵红.山药洋葱营养酒的研究[J].酿酒科技,2009(8):94-96.
[16]杨立朝,周灏,张亚莉,等.辣木茎木乙醇提取物对雄性小鼠性功能的改善作用[J].现代妇女:医学前沿,2014,8:104.
[17]罗琼,黄晓兰,李卓能,等.枸杞多糖对雄性大鼠性功能及生殖功能的影响[J].营养学报,2006,28(1):62-65,70.
[18]罗琼,任世成,杨明亮,等.海带多糖对睾丸受辐射雄性大鼠性功能的影响[J].营养学报,2009,31(3):259-262.
[19]Lagoda G,Muschamp JW,Vigdorchik A,et al.A nitric oxide synthesis inhibitor in the medial preoptic area inhibits copulation and stimulus sensitization in male rats[J].Behavioral Neuroscience,2004,118(6):1317-1323.
[20]Zhang J,Wang YB,Ma CG,et al.Icarisid II,a PDE5 inhibitor from Epimedium wanshanense,increases cellular c GMP by enhancing NOS in diabetic ED rats corpus cavernosum tissue[J].Andrologia,2012,44(1):87-93.
[21]鲍雷,王军波,张远,等.吉林人参低聚肽对雄性小鼠性功能影响的实验研究[J].中国预防医学杂志,2015,16(10):757-760.
[2]李维,兰海楠,郭风,等.生物活性肽的研究进展[J].中国畜牧兽医,2012,39(10):105-107.
[3]陈彩霞,苏秀兰,韩瑞兰.生物活性肽调节免疫功能的研究现状[J].现代预防医学,2016,43(15):2798-2801.
[4]杨韵,徐波.牡蛎的化学成分及其生物活性研究进展[J].中国现代中药,2015,12(12):1345-1349.
[5]江长优,张健,赵江贺.牡蛎多糖增强小鼠免疫功能作用研究[J].中成药,2013,35(5):1062-1065.
[6]吉宏武,苗建银,邵海艳,等.近江牡蛎肉水解物的营养成分及抗疲劳作用研究[J].食品科技,2010,35(2):70-73.
[7]李江滨,侯敢,赖银璇.牡蛎多糖抑制流感病毒增殖的实验研究[J].时珍国医国药,2009,20(6):1346-1347.
[8]秦小明,林华娟,张自然,等.牡蛎肉的醒酒作用机理初探[J].渔业科学进展,2011,32(1):109-113.
[9]陈悦,李路,闫朝阳,等.小分子牡蛎多肽对雄性小鼠性功能的影响[J/OL].(2017-09-04)[2018-05-07).http://kns.cnki.net/kcms/detail/45.1369.Q.20170906.0733.002.html.
[10]杨子明,刘金磊,颜小捷,等.核桃多肽对D-半乳糖诱导老年小鼠血脂水平的影响[J].食品科学,2015,36(9):181-184.
[11]侯雅坤.核桃活性肽体外抗氧化活性及ACE抑制活性的研究[D].北京:北京林业大学,2012.
[12]裴晓惠,张佳程,齐建勋,等.核桃蛋白肽脱苦及其对ACE抑制活性的影响[J].食品工业科技,2012,33(20):236-239.
[13]贾靖霖,蒲云峰,李虎,等.核桃多肽抗疲劳作用的研究[J].食品工业科技,2014,35(7):340-342.
[14]Robbins WA,Xun L,Fitzgerald LZ,et al.Walnuts improve semen quality in men consuming a Western-Style Diet:randomized control dietary intervention trial[J].Biology of Reproduction,2012,87(4):101.
[15]赵贵红.山药洋葱营养酒的研究[J].酿酒科技,2009(8):94-96.
[16]杨立朝,周灏,张亚莉,等.辣木茎木乙醇提取物对雄性小鼠性功能的改善作用[J].现代妇女:医学前沿,2014,8:104.
[17]罗琼,黄晓兰,李卓能,等.枸杞多糖对雄性大鼠性功能及生殖功能的影响[J].营养学报,2006,28(1):62-65,70.
[18]罗琼,任世成,杨明亮,等.海带多糖对睾丸受辐射雄性大鼠性功能的影响[J].营养学报,2009,31(3):259-262.
[19]Lagoda G,Muschamp JW,Vigdorchik A,et al.A nitric oxide synthesis inhibitor in the medial preoptic area inhibits copulation and stimulus sensitization in male rats[J].Behavioral Neuroscience,2004,118(6):1317-1323.
[20]Zhang J,Wang YB,Ma CG,et al.Icarisid II,a PDE5 inhibitor from Epimedium wanshanense,increases cellular c GMP by enhancing NOS in diabetic ED rats corpus cavernosum tissue[J].Andrologia,2012,44(1):87-93.
[21]鲍雷,王军波,张远,等.吉林人参低聚肽对雄性小鼠性功能影响的实验研究[J].中国预防医学杂志,2015,16(10):757-760.