温肾益精散对肾阳虚模型大鼠生长性能的影响
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摘要
为评价温肾益精散在动物生长性能方面的作用,以氢化可的松注射液25 mg/(kg·d)双侧后肢股四头肌交替注射15 d建立肾阳虚大鼠模型。将大鼠随机分为空白对照组,模型组,金匮肾气丸组和温肾益精散高、中、低剂量组。空白对照组和模型组大鼠连续30 d灌服0.9%氯化钠溶液,其余各组大鼠连续30 d灌服相应质量浓度的金匮肾气丸和温肾益精散。检测大鼠30 d平均日增重、平均日采食量,计算其饲料转化率;记录大鼠体质量的变化;用ELISA方法检测血清中生长激素(GH)、生长抑素(SST)和胰岛素样生长因子1(IGF-1)含量,RT-PCR方法检测肝中GHR、JAK2、STAT5b和IGF-1 m RNA表达量。结果显示,与模型组相比,温肾益精散高剂量组大鼠平均日采食量显著降低(P<0.05),饲料转化率提高,体质量升高;温肾益精散中剂量组大鼠平均日增重显著升高(P<0.05),饲料转化率提高,体质量显著升高(P<0.05),血清中GH和IGF-1质量浓度显著升高(P<0.05),SST质量浓度显著降低(P<0.05),肝中JAK2和IGF-1 m RNA表达量显著升高(P<0.05);温肾益精散低剂量组大鼠平均日增重和体质量均显著升高(P<0.05),饲料转化率提高。结果表明,适宜质量浓度的温肾益精散可以改善肾阳虚模型大鼠的生长性能,且这种功能的发挥可能是通过JAK-STAT信号通路完成的。
To evaluate Wenshen Yijing San functions about growth performance in animals,kidneyyang deficiency rat model was established through alternating intramuscular injection in bilateral quadriceps femoris,which lasted for 15 days used hydrocortisone injection.After modeling,rats were divided in six groups randomly,which called blank control group,model group,Jinkui Shenqi Pill group and high,middle,low dose groupsof Wenshen Yijing San.The rats in the blank control group and model group were gavaged 0.9% sodium chlorides solution for 30 days,and the rats in other groups were gavaged the corresponding dose Jinkui Shenqi Pill and Wenshen Yijing San,respectively.The rats' average daily gain(ADG),and average daily feed intake(ADFI) were determinated and the feed conversion rate(FCR) was calculated during 30 days.Rat body weight changes were recorded.Mass concentrations of serum growthhormone(GH),somatostatin(SST) and insulin-like growth factors-1(IGF-1) were detected by ELISA method.The GHR,JAK2,STAT5 b and IGF-1 m RNA expressions in the liver were detected by RT-PCR method.Compared with the model group,high dose of Wenshen Yijing San could significantly decrease the rat ADG(P<0.05),and increase the FCR and the body weight;middle dose of Wenshen Yijing San could significantly increase the rat ADG,the body weight,serum GH,and IGF-1 mass concentration,and the JAK2 and IGF-1 m RNA expression in the liver(P <0.05),increase the FCR,and significantly decrease serum SST mass concentration(P<0.05);low dose of Wenshen Yijing San could significantly increase the rat ADG and the body weight(P<0.05),and increase the FCR.The suitable dose of Wenshen Yijing San can improve the growth performance of kidney-yang deficiency model rats,and this function may be performed through the JAK-STAT signaling pathway.
To evaluate Wenshen Yijing San functions about growth performance in animals,kidneyyang deficiency rat model was established through alternating intramuscular injection in bilateral quadriceps femoris,which lasted for 15 days used hydrocortisone injection.After modeling,rats were divided in six groups randomly,which called blank control group,model group,Jinkui Shenqi Pill group and high,middle,low dose groupsof Wenshen Yijing San.The rats in the blank control group and model group were gavaged 0.9% sodium chlorides solution for 30 days,and the rats in other groups were gavaged the corresponding dose Jinkui Shenqi Pill and Wenshen Yijing San,respectively.The rats' average daily gain(ADG),and average daily feed intake(ADFI) were determinated and the feed conversion rate(FCR) was calculated during 30 days.Rat body weight changes were recorded.Mass concentrations of serum growthhormone(GH),somatostatin(SST) and insulin-like growth factors-1(IGF-1) were detected by ELISA method.The GHR,JAK2,STAT5 b and IGF-1 m RNA expressions in the liver were detected by RT-PCR method.Compared with the model group,high dose of Wenshen Yijing San could significantly decrease the rat ADG(P<0.05),and increase the FCR and the body weight;middle dose of Wenshen Yijing San could significantly increase the rat ADG,the body weight,serum GH,and IGF-1 mass concentration,and the JAK2 and IGF-1 m RNA expression in the liver(P <0.05),increase the FCR,and significantly decrease serum SST mass concentration(P<0.05);low dose of Wenshen Yijing San could significantly increase the rat ADG and the body weight(P<0.05),and increase the FCR.The suitable dose of Wenshen Yijing San can improve the growth performance of kidney-yang deficiency model rats,and this function may be performed through the JAK-STAT signaling pathway.
引文
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