黄连解毒汤对小鼠血清中Trp、Kyn、5-HT及粪便中短链脂肪酸代谢的影响
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摘要
为了探讨黄连解毒汤对KM小鼠血清中色氨酸(Trp)、犬尿氨酸(Kyn)、5-羟色胺(5-HT)和粪便中短链脂肪酸代谢的影响,将KM小鼠随机分为空白对照组(16只)和黄连解毒汤组(16只),通过对小鼠灌胃黄连解毒汤,分别在干预7,14 d后,每组随机处死8只并分别采集粪便和血清样本,运用高效液相色谱法(HPLC)测定血清中Trp、Kyn和5-HT浓度,采用气相色谱法(GC)测定粪便中乙酸、丙酸和丁酸含量。结果表明:与空白对照组相比,黄连解毒汤组小鼠粪便中乙酸、丙酸和丁酸以及血清中Kyn、5-HT浓度均极显著升高(P<0.01),而血清中Trp浓度无显著变化(P>0.05)。说明黄连解毒汤对KM小鼠血清和粪便代谢有一定的影响。
In this study,KM mice were randomly divided into control group(16) and Huanglian Jiedu Decoction group(16). The Huanglian Jiedu Decoction was given to mice by intragastric administration.Eight mice in each group were randomly executed to collect the feces and serum samples on day 7 and 14 post-treatment,respectively.Trp, Kyn, and 5-HT concentrations in serum were detected by HPLC.The content of acetic acid, propionic acid and butyric acid in feces were measured by GC. Results show that the acetic acid, propionic acid and butyric acid in feces and the concentration of Kyn, 5-HT in serum of experimental group are extremly increased than that in the control group(P<0.01).However, there is no significant change in the concentration of Trp between two groups(P>0.05). The results suggest that Huanglian Jiedu Decoction have a certain effect on serum and fecal metabolism of KM mice.
In this study,KM mice were randomly divided into control group(16) and Huanglian Jiedu Decoction group(16). The Huanglian Jiedu Decoction was given to mice by intragastric administration.Eight mice in each group were randomly executed to collect the feces and serum samples on day 7 and 14 post-treatment,respectively.Trp, Kyn, and 5-HT concentrations in serum were detected by HPLC.The content of acetic acid, propionic acid and butyric acid in feces were measured by GC. Results show that the acetic acid, propionic acid and butyric acid in feces and the concentration of Kyn, 5-HT in serum of experimental group are extremly increased than that in the control group(P<0.01).However, there is no significant change in the concentration of Trp between two groups(P>0.05). The results suggest that Huanglian Jiedu Decoction have a certain effect on serum and fecal metabolism of KM mice.
引文
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[14] 李海娜.培元解郁方对慢性应激所致抑郁症的抗抑郁作用及TRP-KYN代谢途径的调节[D].北京:北京中医药大学,2017.
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[18] YU H,LI R,HUANG H,et al.Short-Chain fatty acids enhance the lipid accumulation of 3T3-L1 cells by modulating the expression of enzymes of fatty acid metabolism[J].Lipids,2018,53(1):77-84.
[19] GRIDERJ R,PILAND B E.The peristaltic reflex induced by short-chain fatty acids is mediated by sequential release of 5-HT and neuronal CGRP but not BDNF[J].Am J Physiol Gastrointest Liver Physiol,2007,292(1):G429-437.
[2] 裴亚玲,文英会,李荣誉.黄连解毒汤对抗菌药的抗菌活性的影响[C]//中国畜牧兽医学会兽医药理毒理学分会.中国畜牧兽医学会兽医药理毒理学分会第十一届会员代表大会暨第十三次学术讨论会与中国毒理学会兽医毒理专业委员会第五次学术研讨会论文集.[出版地不详:出版者不详],2015.
[3] 涂春联,李红胜.黄连解毒汤治疗肥胖型2型糖尿病临床研究[J].中医学报,2015,30(5):644-646.
[4] 孙燕,万冰,金戈,等.黄连解毒汤对小鼠S180肉瘤血管生成抑制作用的试验研究[J].东北农业大学学报,2014,45(12):31-35.
[5] 林丽丽,汪受传,杨燕,等.基于代谢组学方法的小儿肺炎易感性研究[J].分析化学,2018,46(2):188-197.
[6] 陈志敏,胡昌江,郑午,等.代谢组学在中药炮制机制研究中的应用概述[J].中华中医药学刊,2018,36(2):275-279.
[7] 龚梦鹃,李春苑,巫圣乾,等.藿香正气口服液干预湿困脾胃证大鼠的血清和粪便代谢组学研究[J].中草药,2017,48(14):2889-2894.
[8] 贾波,李翼.方剂学[M].上海:科学技术出版社,2012.
[9] 陈奇.中药药理研究方法学[M].北京:人民卫生出版社,1993.
[10] 陈瑞,吴云,段智璇,等.运用同一波长HPLC法测定大鼠血清色氨酸及其代谢产物[J].贵阳中医学院学报,2016,38(1):11-15.
[11] 刘二东,李志伟,杨更亮,等.谱图叠加法用于色谱微弱信号的检测[J].河北大学学报(自然科学版),2003,24(4):392-394.
[12] 孙彩艳,张天栋,武德珍,等.外标一点法在人体甲氨蝶呤血浆浓度测定中的应用[J].中国医院用药评价与分析,2014,14(11):982-984.
[13] 明建,吴素蕊,曾凯芳,等.羊肚菌多糖PMEP-1对大鼠肠道内短链脂肪酸的影响[J].食品科学,2010,31(19):367-371.
[14] 李海娜.培元解郁方对慢性应激所致抑郁症的抗抑郁作用及TRP-KYN代谢途径的调节[D].北京:北京中医药大学,2017.
[15] LE F N,SIMONGIOVANNI A,CORRENT E,et al.Comparison of plasma tryptophan-related metabolites in crossbred Piétrain and Duroc pigs[J].J Anim Sci,2017,95(4):1606-1613.
[16] 卞秀梅,吴正祥,陶科明,等.血清IDO和KYN在溃疡性结肠炎中的临床价值研究[J].安徽医科大学学报,2013,48(12):1484-1487.
[17] LAYUNTA E,LATORRE E,FORCEEN R,et al.NOD1 downregulates intestinal serotonin transporter and interacts with other pattern recognition receptors[J].J Cell Physiol,2018,233(5):4183-4193.
[18] YU H,LI R,HUANG H,et al.Short-Chain fatty acids enhance the lipid accumulation of 3T3-L1 cells by modulating the expression of enzymes of fatty acid metabolism[J].Lipids,2018,53(1):77-84.
[19] GRIDERJ R,PILAND B E.The peristaltic reflex induced by short-chain fatty acids is mediated by sequential release of 5-HT and neuronal CGRP but not BDNF[J].Am J Physiol Gastrointest Liver Physiol,2007,292(1):G429-437.