壳假网衣共生藻对温度变化的响应
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摘要
以产于山西管涔山地区的壳假网衣(Porpidia crustulata)为材料,对其共生藻在不同温度培养条件下的几个与抗性有关的生理指标进行了研究。结果显示,壳假网衣共生藻在5℃和10℃低温培养条件下3d后MDA含量逐渐减少,培养初期共生藻的SOD活力和可溶性糖含量较高,培养12d后可溶性蛋白质的量远高于培养初期。研究结果表明,地衣共生藻在长期的共生关系中可能建立了对低温的适应机制,表现出了较好的抗冷性。
The physiological indexes(MDA,SOD,soluble sugar and soluble protein)of algal partners associated with the lichen-forming fungus Porpidia crustulata from Guancen Mountains(Chlorella sp.GC)were studied at different temperatures.The results showed that the MDA content of Chlorellasp.GC was gradual decline when they were cultured for 3dat 5℃and 10℃.In the early stage of culture,the SOD activity and soluble sugar content of Chlorellasp.GC were much higher than they were cultured.The content of soluble protein of Chlorellasp.That of GC was less than after cultured for 12 d.The results indicated that the mechanism of low-temperature adaptation of Chlorellasp.GC was formed in a long-term symbiotic relationship and Chlorellasp.That of GC showed distinct cold hardiness.
The physiological indexes(MDA,SOD,soluble sugar and soluble protein)of algal partners associated with the lichen-forming fungus Porpidia crustulata from Guancen Mountains(Chlorella sp.GC)were studied at different temperatures.The results showed that the MDA content of Chlorellasp.GC was gradual decline when they were cultured for 3dat 5℃and 10℃.In the early stage of culture,the SOD activity and soluble sugar content of Chlorellasp.GC were much higher than they were cultured.The content of soluble protein of Chlorellasp.That of GC was less than after cultured for 12 d.The results indicated that the mechanism of low-temperature adaptation of Chlorellasp.GC was formed in a long-term symbiotic relationship and Chlorellasp.That of GC showed distinct cold hardiness.
引文
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[22]郭惠红.金边卫矛低温抗性研究[D].北京:北京林业大学,2004.
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[2]康俊梅.低温胁迫下野牛草生理生化响应及蛋白质组学研究[D].北京:中国农业科学院,2008.
[3]冯建灿,张玉洁,杨天柱.低温胁迫对喜树幼苗SOD活性、MDA和脯氨酸含量的影响[J].林业科学研究,2002,15(2):197-202.
[4]Chris B,Marc V H,Dirk I.Superoxide Dismutase and Stress Tolerance[J].Annu Rev Plant Pysiol Plant Mol Biol,1992,43:83.
[5]严寒静,谈锋.桅子叶片生理特性与抗寒性的关系[J].植物资源与环境学报,2005,14(4):21-24.
[6]戴玉池,邓霞玲,姜孝成,等.不同水稻品种幼苗期的耐寒生理鉴定及其利用[J].湖南师范大学:自然科学学报,2004,27(3):86-89.
[7]王静,杨持.冷篙抗寒生理特性的研究[J].内蒙古大学学报:自然科学版,2002,33(6):673-676.
[8]Li B,Feng J,Xie S.Morphological and Phylogenetic Study of Algal Partners Associated with the Lichen-forming Fungus Porpidia Crustulata from the Guancen Mountains,northern China[J].Symbiosis,2013,61(1):37-46.
[9]Li B,Feng J,Xie S.Relationship Between the Algal Partners and the Growth of Lichen-forming Fungus Porpidia Crustulata[J].Symbiosis,2014,62(2):101-109.
[10]彭金光,孙玉宏,师瑞红,等.10℃低温对西瓜幼苗耐性生理指标的影响[J].安徽农学通报,2006,12(10):42-45.
[11]朱政,蒋家月,江昌俊,等.低温胁迫对茶树叶片SOD,可溶性蛋白和可溶性糖含量的影响[J].安徽农业大学学报,2011,38(1):24-26.
[12]Anderson I C,Robertson D S.Role of Carotenoids in Protecting Chlorophyll from Photodestruction[J].Plant Physiol,1960,35(4):531.
[13]滕中华,周党卫,师生波,等.青藏高原三种高寒植物的质膜透性变化与抗寒性的关系[J].中国草地,2001,23(4):37-47.
[14]严寒静,谈锋.自然降温过程中桅子叶片膜保护系统的变化与低温半致死温度的关系[J].植物生态学报,2000,24(1):91-95.
[15]王善广,张华云,郭郑,等.生物膜与果树抗寒性[J].天津农业科学,2000,6(1):37-40.
[16]杨亚军,郑雷英,王新超.低温对茶树叶片膜脂脂肪酸和蛋白质的影响[J].亚热带植物科学,2005,34(1):5-9.
[17]宋凤鸣,葛秀春,郑重.活性氧及膜脂过氧化与棉花对枯萎病抗性的关系[J].植物病理学报,2001,5(2):110-116.
[18]路丙社,白志英,李会平,等.水分胁迫对阿月浑子幼苗叶片膜脂过氧化和膜磷脂脱酷化反应的影响[J].果树学报,2004,21(1):33-36.
[19]杨盛昌,谢潮添,张平,等.低温胁迫下弓葵幼苗膜脂过氧化及保护酶活性的变化[J].园艺学报,2003,30(1):104-106.
[20]李瀚海,尹飞,王群.不同耐旱性玉米杂交种及其亲本叶片活性氧代谢对水分胁迫的响应[J].生态学报,2006,26(6):1912-1918.
[21]武维华,张蜀秋,等.植物生理学[M].北京:科学出版社,2003:188-189.
[22]郭惠红.金边卫矛低温抗性研究[D].北京:北京林业大学,2004.
[23]Thüs H,Muggia L,Pérez-Ortega S,et al.Revisiting Photobiont Diversity in the Lichen Family Verrucariaceae(Ascomycota)[J].Eur J Phycol,2011,46(4):399-415.