骨膜蛋白和p53在口腔白斑及鳞状细胞癌组织中的表达及意义
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摘要
目的探讨骨膜蛋白和p53在口腔白斑和口腔鳞状细胞癌组织中的表达、相关性及临床意义。方法采用免疫组织化学法检测30例口腔正常黏膜、30例单纯增生白斑、38例异常增生白斑以及79例口腔鳞状细胞癌组织中骨膜蛋白和p53的表达情况,并分析表达的相关性。结果对于骨膜蛋白阳性细胞面积指数和累积光密度值,除单纯增生与正常口腔黏膜的差异无统计学意义外,其余各组间的差异均有统计学意义(P<0.05);对于p53阳性细胞面积指数和累积光密度值,除单纯增生白斑与正常口腔黏膜的差异无统计学意义外,其余各组间的差异均有统计学意义(P<0.05)。异常增生白斑和口腔鳞状细胞癌组织中骨膜蛋白与p53阳性表达呈线性正相关(P<0.05)。骨膜蛋白和p53的阳性表达与肿瘤的分化程度、肿瘤TNM分期以及有无淋巴结转移有关(P<0.05)。结论骨膜蛋白和p53可能共同参与口腔鳞状细胞癌的癌变过程,骨膜蛋白和p53与肿瘤侵袭有关。
Objective This study aimed to investigate the expression and clinical significance of periostin and p53 in oral leukoplakia and oral squamous cell carcinoma. Methods The material for immunohistochemical studies consisted of 30 cases of normal oral mucosa, 30 cases of hyperplasia leukoplakia, 38 cases of dysplastic leukoplakia and 79 cases of oral squamous cell carcinoma. Results The index of positive cell vessels and the value of integrated optical density of periostin and p53 between every two groups, except for oral normal mucosa and hyperplasia leukoplakia, were statistically significant(P<0.05). Periostin expression in dysplastic leukoplakia and oral squamous cell carcinoma positively correlated with that of p53. Furthermore,the expression levels of periostin and p53 were significantly related to TNM stage,tumour differentiation degree and lymph node metastasis(P<0.05). Conclusion Periostin and p53 may be involved in the carcinogenesis of oral squamous cell carcinoma and associated with tumour invasion.
Objective This study aimed to investigate the expression and clinical significance of periostin and p53 in oral leukoplakia and oral squamous cell carcinoma. Methods The material for immunohistochemical studies consisted of 30 cases of normal oral mucosa, 30 cases of hyperplasia leukoplakia, 38 cases of dysplastic leukoplakia and 79 cases of oral squamous cell carcinoma. Results The index of positive cell vessels and the value of integrated optical density of periostin and p53 between every two groups, except for oral normal mucosa and hyperplasia leukoplakia, were statistically significant(P<0.05). Periostin expression in dysplastic leukoplakia and oral squamous cell carcinoma positively correlated with that of p53. Furthermore,the expression levels of periostin and p53 were significantly related to TNM stage,tumour differentiation degree and lymph node metastasis(P<0.05). Conclusion Periostin and p53 may be involved in the carcinogenesis of oral squamous cell carcinoma and associated with tumour invasion.
引文
[1]中华口腔医学会口腔黏膜病专业委员会.口腔白斑病的定义与分级标准(试行)[J].中华口腔医学杂志,2011,46(10):579-580.Chinese Stomatological Association Oral Mucosal Disease Committee. Oral leukoplakia definition and grading standards[J]. Chin J Stomatol, 2011, 46(10):579-580.
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[7]姜洋,金晓明,屠康.平均阳性染色面积百分比法分析免疫组化结果初探[J].生物医学工程学杂志,2007, 24(3):650-653.Jiang Y, Jin XM, Tu K. A primary study using the method of average positive stained area percentage to measure the immunohistochemistry result[J]. J Biomed Eng,2007, 24(3):650-653.
[8]汤根兄,吴国英,李静.Stat3和cyclinDl在口腔黏膜癌前病变和口腔鳞癌中的表达[J].口腔医学研究,2011,27(1):40-43.Tang GX, Wu GY, Li J. Stat3 and cyclinD1 expression in oral mucosal precancerous lesions and oral squamous cell carcinoma[J]. J Oral Sci Res, 2011,27(1):40-43.
[9] Eckert AW, Wickenhauser C, Salins PC, et al. Clinical relevance of the tumor microenvironment and immune escape of oral squamous cell carcinoma[J]. J Transl Med, 2016, 14:85.
[10]胡汪来,吴缅.p53在肿瘤发生过程中的功能研究及进展[J].中国科学:生命科学,2017, 47(1):52-58.Hu WL, Wu M. Study on the function of p53 in tumorigenesis and its progress[J]. Scienta Sinica Vitae,2017, 47(1):52-58.
[11] Hong Q, Ke C, Ji Y, et al. Expression and clinicopathologic significance of TUFM and p53 for the normal-adenoma-carcinoma sequence in colorectal epithelia[J]. World J Surg Oncol, 2017, 15(16):238-240.
[12] Tilman G,Mattiussi M, Brasseur F,et al. Human periostin gene expression in normal tissues, tumors and melanoma:evidences for periostin production by both stromal and melanoma cells[J]. Mol Cancer,2007, 6:80.
[13] Koh SJ, Choi Y, Kim BG, et al. Matricellular protein periostin mediates intestinal inflammation through the activation of nuclear factorκB signaling[J]. PLoS One, 2016, 11(2):e0149652.
[14] Isono T,Kim CJ, Ando Y,et al. Suppression of cell invasiveness by periostin via TAB1/TAK1[J]. Int J Oncol, 2009, 35(2):425-432.
[15] Kanno A, Satoh K,Masamune A,et al. Periostin,secreted from stromal cells, has biphasic effect on cell migration and correlates with the epithelial to mesenchymal transition of human pancreatic cancer cells[J]. Int J Cancer, 2008, 122(12):2707-2718.
[16] Moniuszko T, Wincewicz A, Koda M, et al. Role of periostin in esophageal, gastric and colon cancer[J].Oncol Lett,2016, 12(2):783-787.
[17] Landre V, Antonov A, Knight R, et al. p73 promotes glioblastoma cell invasion by directly activating POSTN(periostin)expression[J]. Oncotarget, 2016,7(11):11785-11802.
[18] Lister NC,Clemson M,Morris KV. RNA-directed epigenetic silencing of periostin inhibits cell motility[J]. R Soc Open Sci,2015, 2(6):140545.
[19] Wang W, Sun QK, He YF, et al. Overexpression of periostin is significantly correlated to the tumor angiogenesis and poor prognosis in patients with esophageal squamous cell carcinoma[J]. Int J Clin Exp Pathol,2014, 7(2):593-601.
[20] Sun L, Fang J. Epigenetic regulation of epithelialmesenchymal transition[J]. Cell Mol Life Sci, 2016,73(23):4493-4515.
[2] Neville BW, Day TA. Oral cancer and precancerous lesions[J]. CA Cancer J Clin, 2002, 52(4):195-215.
[3] Lv H,Liu R, Fu J,et al. Epithelial cell-derived periostin functions as a tumor suppressor in gastric cancer through stabilizing p53 and E-cadherin proteins via the Rb/E2F1/p14ARF/Mdm2 signaling pathway[J]. Cell Cycle,2014, 13(18):2962-2974.
[4]龙小佳,何力,王璐.HPV16E6和p53在口腔癌中的表达及相关性研究[J].第三军医大学学报,2016,38(21):2326-2329.Long XJ, He L, Wang L. Expression of HPV16E6and p53 in oral cance[J]. J Third Mil Med Univ,2016,38(21):2326-2329.
[5] Li B, Wang L, Chi B. Upregulation of periostin prevents P53-mediated apoptosis in SGC-7901 gastric cancer cells[J]. Mol Biol Rep, 2013, 40(2):1677-1683.
[6] Michaylira CZ, Wong GS, Miller CG, et al. Periostin,a cell adhesion molecule, facilitates invasion in the tumor microenvironment and annotates a novel tumor-invasive signature in esophageal cancer[J].Cancer Res, 2010, 70(13):5281-5292.
[7]姜洋,金晓明,屠康.平均阳性染色面积百分比法分析免疫组化结果初探[J].生物医学工程学杂志,2007, 24(3):650-653.Jiang Y, Jin XM, Tu K. A primary study using the method of average positive stained area percentage to measure the immunohistochemistry result[J]. J Biomed Eng,2007, 24(3):650-653.
[8]汤根兄,吴国英,李静.Stat3和cyclinDl在口腔黏膜癌前病变和口腔鳞癌中的表达[J].口腔医学研究,2011,27(1):40-43.Tang GX, Wu GY, Li J. Stat3 and cyclinD1 expression in oral mucosal precancerous lesions and oral squamous cell carcinoma[J]. J Oral Sci Res, 2011,27(1):40-43.
[9] Eckert AW, Wickenhauser C, Salins PC, et al. Clinical relevance of the tumor microenvironment and immune escape of oral squamous cell carcinoma[J]. J Transl Med, 2016, 14:85.
[10]胡汪来,吴缅.p53在肿瘤发生过程中的功能研究及进展[J].中国科学:生命科学,2017, 47(1):52-58.Hu WL, Wu M. Study on the function of p53 in tumorigenesis and its progress[J]. Scienta Sinica Vitae,2017, 47(1):52-58.
[11] Hong Q, Ke C, Ji Y, et al. Expression and clinicopathologic significance of TUFM and p53 for the normal-adenoma-carcinoma sequence in colorectal epithelia[J]. World J Surg Oncol, 2017, 15(16):238-240.
[12] Tilman G,Mattiussi M, Brasseur F,et al. Human periostin gene expression in normal tissues, tumors and melanoma:evidences for periostin production by both stromal and melanoma cells[J]. Mol Cancer,2007, 6:80.
[13] Koh SJ, Choi Y, Kim BG, et al. Matricellular protein periostin mediates intestinal inflammation through the activation of nuclear factorκB signaling[J]. PLoS One, 2016, 11(2):e0149652.
[14] Isono T,Kim CJ, Ando Y,et al. Suppression of cell invasiveness by periostin via TAB1/TAK1[J]. Int J Oncol, 2009, 35(2):425-432.
[15] Kanno A, Satoh K,Masamune A,et al. Periostin,secreted from stromal cells, has biphasic effect on cell migration and correlates with the epithelial to mesenchymal transition of human pancreatic cancer cells[J]. Int J Cancer, 2008, 122(12):2707-2718.
[16] Moniuszko T, Wincewicz A, Koda M, et al. Role of periostin in esophageal, gastric and colon cancer[J].Oncol Lett,2016, 12(2):783-787.
[17] Landre V, Antonov A, Knight R, et al. p73 promotes glioblastoma cell invasion by directly activating POSTN(periostin)expression[J]. Oncotarget, 2016,7(11):11785-11802.
[18] Lister NC,Clemson M,Morris KV. RNA-directed epigenetic silencing of periostin inhibits cell motility[J]. R Soc Open Sci,2015, 2(6):140545.
[19] Wang W, Sun QK, He YF, et al. Overexpression of periostin is significantly correlated to the tumor angiogenesis and poor prognosis in patients with esophageal squamous cell carcinoma[J]. Int J Clin Exp Pathol,2014, 7(2):593-601.
[20] Sun L, Fang J. Epigenetic regulation of epithelialmesenchymal transition[J]. Cell Mol Life Sci, 2016,73(23):4493-4515.