一株DDT降解菌株D3的安全评估
|
摘要
[目的]评估DDT降解菌株D3的生物安全性.[方法]以两种毒理学方法(急性毒性试验和亚慢性毒性试验)评估DDT降解菌株D3对哺乳动物的安全性.[结果]急性毒性试验中,大鼠未出现明显中毒症状和死亡情况,且DDT降解菌株D3对大鼠的口服半数致死量(median lethal dose,LD50)超过5000 mg/kg(6. 3×1011CFU/kg);亚慢性毒性试验中,试验各组与空白对照组在相关指标对比中无明显差异.[结论]DDT降解菌株D3对哺乳动物安全无毒副作用,可以作为治理DDT污染土壤的修复剂推广应用.
This study is aimed to evaluate of the biosafety of DDT degrading bacteria D3. Two toxicological methods(acute toxicity test and subchronic toxicity test) were used to evaluate the safety of DDT degrading strain D3 in mammals. Results showed that there were no significant signs of toxicity and mortality in acute toxicity study,and the oral LD50 of DDT degrading bacteria D3 was more than 5000 mg/kg(6. 3 × 1011 CFU/kg). In the subchronic toxicity test,there was no significant difference between the test groups and the blank control group in the correlation indexes. It is concluded that DDT degrading bacteria D3 has no toxic and side effects to mammals and can be used as a remediation agent for DDT contaminated soil.
This study is aimed to evaluate of the biosafety of DDT degrading bacteria D3. Two toxicological methods(acute toxicity test and subchronic toxicity test) were used to evaluate the safety of DDT degrading strain D3 in mammals. Results showed that there were no significant signs of toxicity and mortality in acute toxicity study,and the oral LD50 of DDT degrading bacteria D3 was more than 5000 mg/kg(6. 3 × 1011 CFU/kg). In the subchronic toxicity test,there was no significant difference between the test groups and the blank control group in the correlation indexes. It is concluded that DDT degrading bacteria D3 has no toxic and side effects to mammals and can be used as a remediation agent for DDT contaminated soil.
引文
[1]Kim S.,Park J.,Kim H.J.,et al.Association between Several Persistent Organic Pollutants in Serum and Adipokine Levels in Breast Milk among Lactating Women of Korea[J].Environmental Science&Technology,2015,49(13):8033-8040.
[2]罗飞,宋静,潘云雨,等.典型滴滴涕废弃生产场地污染土壤的人体健康风险评估研究[J].土壤学报,2012,49(1):26-35.
[3]Bibiana B.C.,Nancy J.P.,Santiago C.,et al.Evaluation of biostimulation and Tween 80 addition for the bioremediation of longterm DDT-contaminated soil[J].Journal of environmental sciences,2015,28:101-109.
[4]Abdul G.,Masaaki T.,Azhar M.,et al.Efficient DDT and trichlorophenol detoxification using Na BH4 and Devarda alloy[J].Environmental chemistry letter,2013,11:197-202.
[5]欧丽,李义连.武汉某废弃农药厂土壤中DDT及其代谢产物的空间分布特征研究[J].安全与环境工程,2014,21(2):46-50.
[6]张明星,洪青,何健,等.DDT降解菌株DB-1的分离、系统发育及降解特性[J].中国环境科学,2005,25(6):674-677.
[7]李红权,李红梅,蒋继志,等.一株DDT降解菌的筛选、鉴定及降解特性的初步研究[J].微生物学通报,2008,35(5):696-699.
[8]朱丹,关健飞,张珊珊,等.一株DDT降解菌的鉴定及其生物学特性[J].安全与环境学报,2016,16(2):319-323.
[9]Lakshmi S.G.,Jayanthi N.,Saravanan M.,et al.Safety assesment of Bacillus clausii UBBC07,a spore forming probiotic[J].Toxicology Reports,2017,4(2):62-71.
[10]李建科.食品毒理学[M].北京:中国计量出版社,2012,12:132-134.
[11]Chowdhury S.P.,Uhl J.,Grosch R.,et al.Cyclic Lipopeptides of bacillus amyloliquefaciens subsp.plantarum colonizing the lettuce rhizosphere enhance plant defense responses toward the bottom rot pathogen rhizoctonia solani[J].Molecular plant-microbe interactions,2015,28(9):984-995.
[12]王心如.毒理学实验方法与技术[M].北京:人民卫生出版社,2014,12:36-50.
[13]Olson H.,Betton G.,Robinson D.,et al.Concordance of toxicology of pharmaceuticals in humans and animals[J].Regulatory toxicology and pharmacology,2000,32:56-67.
[14]Mukinda J.,Syce J.A..Acute and chronic toxicity of aqueous extract of Artemisia afra in rodents[J].Journal of Ethnopharmacology,2007,112:138-144.
[15]Ramaiah S.K.Preclinical safety assessment:current gaps,challenges,and approaches in identifying translatable biomarkers of drug-induced liver injury[J].Clinics in Laboratory Medicine,2011,31(1):161-172.
[16]Ozer J.,Ratweb M.,Shawc M.,et al.The current state of serum biomarker of hepatoxicity[J].Toxicity,2008,245(3):194-205.
[17]Evan G.O..Animal clinical chemistry:A Practical Guide for Toxicologists and Biomedical Researchers[M].CRC Press,Boca Raton,pp.2009:321-331.
[18]Rasekh H.R.,Nazari P.,Kamli-Nejad M.,et al.Acute and subchronic oral toxicity of Galega officinalis in rats[J].Journal of Ethnopharmacology,2008,116(1):21-26.
[19]Wang X.,Su S.,Ihsan A.,et al.Acute and sub-chronic toxicity study of diaveridine in Wistar rats[J].Regulatory Toxicology&Pharmacology,2015,73(1):232-240.
[20]Jafarpour D.,Shekarforoush S.S.,Ghaisari H.R.,et al.Protective effects of synbiotic diets of Bacillus coagulans,Lactobacillus plantarum and inulin against acute cadmium toxicity in rats[J].Bmc Complementary&Alternative Medicine,2017,17(1):291-298.
[21]Tortora,G.J.,Derrickson,B..Principles of Anatomy and Physiology[M].John Wiley&Sons,Inc.,United States of America,2009:1047-1048.
[22]臧路平,刘志刚,吴新荣.高尿酸血症发病机制及其药物治疗研究进展[J].医药导报,2011,30(1):69-73.
[23]孔宪如,史光英,徐志刚,等.2型糖尿病患者尿蛋白排泄率与肌酐清除率关系分析[J].临床内科杂志,2010,2(27):114-116.
[24]向丽华,陈燕萍,张智,等.24味有毒中药长期毒性实验对大鼠脏器指数的影响[J].中国中医基础医学杂志,2006,12(1):35-36,52.
[2]罗飞,宋静,潘云雨,等.典型滴滴涕废弃生产场地污染土壤的人体健康风险评估研究[J].土壤学报,2012,49(1):26-35.
[3]Bibiana B.C.,Nancy J.P.,Santiago C.,et al.Evaluation of biostimulation and Tween 80 addition for the bioremediation of longterm DDT-contaminated soil[J].Journal of environmental sciences,2015,28:101-109.
[4]Abdul G.,Masaaki T.,Azhar M.,et al.Efficient DDT and trichlorophenol detoxification using Na BH4 and Devarda alloy[J].Environmental chemistry letter,2013,11:197-202.
[5]欧丽,李义连.武汉某废弃农药厂土壤中DDT及其代谢产物的空间分布特征研究[J].安全与环境工程,2014,21(2):46-50.
[6]张明星,洪青,何健,等.DDT降解菌株DB-1的分离、系统发育及降解特性[J].中国环境科学,2005,25(6):674-677.
[7]李红权,李红梅,蒋继志,等.一株DDT降解菌的筛选、鉴定及降解特性的初步研究[J].微生物学通报,2008,35(5):696-699.
[8]朱丹,关健飞,张珊珊,等.一株DDT降解菌的鉴定及其生物学特性[J].安全与环境学报,2016,16(2):319-323.
[9]Lakshmi S.G.,Jayanthi N.,Saravanan M.,et al.Safety assesment of Bacillus clausii UBBC07,a spore forming probiotic[J].Toxicology Reports,2017,4(2):62-71.
[10]李建科.食品毒理学[M].北京:中国计量出版社,2012,12:132-134.
[11]Chowdhury S.P.,Uhl J.,Grosch R.,et al.Cyclic Lipopeptides of bacillus amyloliquefaciens subsp.plantarum colonizing the lettuce rhizosphere enhance plant defense responses toward the bottom rot pathogen rhizoctonia solani[J].Molecular plant-microbe interactions,2015,28(9):984-995.
[12]王心如.毒理学实验方法与技术[M].北京:人民卫生出版社,2014,12:36-50.
[13]Olson H.,Betton G.,Robinson D.,et al.Concordance of toxicology of pharmaceuticals in humans and animals[J].Regulatory toxicology and pharmacology,2000,32:56-67.
[14]Mukinda J.,Syce J.A..Acute and chronic toxicity of aqueous extract of Artemisia afra in rodents[J].Journal of Ethnopharmacology,2007,112:138-144.
[15]Ramaiah S.K.Preclinical safety assessment:current gaps,challenges,and approaches in identifying translatable biomarkers of drug-induced liver injury[J].Clinics in Laboratory Medicine,2011,31(1):161-172.
[16]Ozer J.,Ratweb M.,Shawc M.,et al.The current state of serum biomarker of hepatoxicity[J].Toxicity,2008,245(3):194-205.
[17]Evan G.O..Animal clinical chemistry:A Practical Guide for Toxicologists and Biomedical Researchers[M].CRC Press,Boca Raton,pp.2009:321-331.
[18]Rasekh H.R.,Nazari P.,Kamli-Nejad M.,et al.Acute and subchronic oral toxicity of Galega officinalis in rats[J].Journal of Ethnopharmacology,2008,116(1):21-26.
[19]Wang X.,Su S.,Ihsan A.,et al.Acute and sub-chronic toxicity study of diaveridine in Wistar rats[J].Regulatory Toxicology&Pharmacology,2015,73(1):232-240.
[20]Jafarpour D.,Shekarforoush S.S.,Ghaisari H.R.,et al.Protective effects of synbiotic diets of Bacillus coagulans,Lactobacillus plantarum and inulin against acute cadmium toxicity in rats[J].Bmc Complementary&Alternative Medicine,2017,17(1):291-298.
[21]Tortora,G.J.,Derrickson,B..Principles of Anatomy and Physiology[M].John Wiley&Sons,Inc.,United States of America,2009:1047-1048.
[22]臧路平,刘志刚,吴新荣.高尿酸血症发病机制及其药物治疗研究进展[J].医药导报,2011,30(1):69-73.
[23]孔宪如,史光英,徐志刚,等.2型糖尿病患者尿蛋白排泄率与肌酐清除率关系分析[J].临床内科杂志,2010,2(27):114-116.
[24]向丽华,陈燕萍,张智,等.24味有毒中药长期毒性实验对大鼠脏器指数的影响[J].中国中医基础医学杂志,2006,12(1):35-36,52.