黄芪心血管药理作用研究进展
|
摘要
黄芪成分较为复杂,且各成分间相互作用后如何影响心血管系统的研究较少;在众多补气药材中黄芪被称为"补气圣药",与其他补气药在药理作用方面必然存在着共性及区别,需要更多的对比研究来诠释黄芪"补气之长"的理论。随着现代药物提取工艺和科学技术的飞速发展,还可以在细胞学、分子学等领域开展相关研究,使黄芪及其有效成分提取物在心血管系统中的药理学作用机制更加明确,以指导临床应用和新药研发。
The application of Huangqi( Radix Astragali) has a long history. Experimental studies have found that Huangqi has protective effects on ischemic myocardial cells,myocardial ischemia-reperfusion injury and myocardial injury caused by virus infection. It also promotes the regeneration of damaged myocardium,and has definite cardiac strengthening,inhibiting ventricular remodeling,regulating blood pressure and regulating blood viscosity. Although there have been a lot of experiments on the analysis of Huangqi and great progress has been made in exploring the mechanism of its chemical constituents,the components of Huangqi are relatively complex,and there are few studies on how to influence the cardiovascular system after the interaction between the components. Huangqi is called"the sacred tonifying medicine"in many medicines for invigorating qi. There must be similarities and differences in pharmacological action between Huangqi and other drugs for invigorating qi. Morecomparative studies are needed to explain the theory of"invigorating qi"of Huangqi. In addition,with the rapid development of modern drug extraction technology and science and technology,related research can also be carried out in the fields of cytology and molecular science.
The application of Huangqi( Radix Astragali) has a long history. Experimental studies have found that Huangqi has protective effects on ischemic myocardial cells,myocardial ischemia-reperfusion injury and myocardial injury caused by virus infection. It also promotes the regeneration of damaged myocardium,and has definite cardiac strengthening,inhibiting ventricular remodeling,regulating blood pressure and regulating blood viscosity. Although there have been a lot of experiments on the analysis of Huangqi and great progress has been made in exploring the mechanism of its chemical constituents,the components of Huangqi are relatively complex,and there are few studies on how to influence the cardiovascular system after the interaction between the components. Huangqi is called"the sacred tonifying medicine"in many medicines for invigorating qi. There must be similarities and differences in pharmacological action between Huangqi and other drugs for invigorating qi. Morecomparative studies are needed to explain the theory of"invigorating qi"of Huangqi. In addition,with the rapid development of modern drug extraction technology and science and technology,related research can also be carried out in the fields of cytology and molecular science.
引文
[1]国家药典委员会.中华人民共和国药典(一部)[M].北京:化学工业出版社,2005:212-213.
[2]温燕梅.黄芪的化学成分研究进展[J].中成药,2006,28(6):879-883.
[3]石刚刚,陈锦香,李长朝,等.黄芪注射液对冠状动脉直接作用的研究[J].中药新药与临床药理,1999,10(1):38-39.
[4]雷春利,陈羽,吕文伟,等.黄芪总皂苷对犬急性心肌梗塞缺血面积、心外膜电图、心肌酶的影响[J].白求恩医科大学学报,1995,21(2):111-113.
[5]闵清,白育庭,劳超,等.黄芪多糖对大鼠心肌缺血保护作用及其机制研究[J].时珍国医国药,2011,22(4):921-923.
[6]关凤英,李红,于秀霞,等.黄芪注射液预适应对大鼠实验性心肌缺血再灌注损伤的保护作用及其机制[J].中国老年学杂志,2010,30(21):3126-3129.
[7]周吉燕,樊懿,孔建龙,等.黄芪中不同提取成分对在体大鼠心肌缺血-再灌注损伤的心功能影响[J].中国中药杂志,2000,25(5):300.
[8]李铁成,张静峰,马宝丰,等.黄芪多糖对心肌缺血再灌注损伤的保护作用[J].天津中医药,2014,31(8):490-492.
[9]王媛媛,彭洋,张琦,等. ERK1/2信号通路对黄芪苷Ⅳ抗H2O2诱导H9c2细胞氧化损伤的作用[J].中国应用生理学杂志,2011,27(3):363-367.
[10]张琦,彭洋,刘艳霞,等.黄芪苷Ⅳ通过PI3K/Akt信号通路抗H2O2诱导的H9c2细胞氧化损伤[J].中草药,2010,41(6):955-959.
[11] HU J Y,HAN J,CHU Z G,et al. AstragalosideⅣattenuates hypoxia-induced cardiomyocyte damage in rats by upregulating superoxide dismutase-1 levels[J]. Clin Exp Pharmacol Physiol,2009,36(4):351-3571.
[12] JONES S P,TROCHA S D,STRANGE M B,et al. Leukocyte and endothelial cell adhesion molecules in a chronic murine model of myocardial reperfusion injury[J]. American Journal of Physiology,2000,279(5):2196-2201.
[13] LUO S F,FANG R Y,HSIEH H L,et al. Involvement of MAPKs and NF-KB in tumor necrosis factorα–induced vascular cell adhesion molecule 1 expression in human rheumatoid arthritis synovial fibroblasts[J]. Arthritis and Rheumatism,2010,62(1):105-116.
[14] MATSUMOTO T,TURESSON I,BOOK M,et al. p38 MAP kinase negatively regulates endothelial cell survival,proliferation,and differentiation in FGF-2-stimulated angiogenesis[J].Journal of Cell Biology,2002,156(1):149-160.
[15] HAI Y Z,YONG H G,ZHI Y W,et al. Astragalus Polysaccharide Suppresses the Expression of Adhesion Molecules through the Regulation of the p38 MAPK Signaling Pathway in Human Cardiac Microvascular Endothelial Cells after Ischemia-Reperfusion Injury[J]. Evid Based Complement Alternat Med,2013,66(4):280-293.
[16]茵涛,杨英珍,杨学义,等.黄芪对小鼠急性病毒性心肌炎作用的心肌细胞电生理研究[J].中国中西医结合杂志,1994,14(5):292-294.
[17]郭棋,彭天庆,杨英珍,等.黄芪对柯萨奇B_3病毒感染培养大鼠心肌细胞Ca2+内流及该病毒RNA复制[J].中国中西医结合杂志,1995,15(8):483-485.
[18] ZHANG Y I,HU G,LIN H C,et al. Radix astragali extract promotes angiogenesis involving vascular endothelial growth factor re-ceptor-related phosphatidylinositol 3-Kinase/Ak t-dependentpathway in human endothelial cells[J]. Phytother Res,2009,23(9):1205-1213.
[19] ZHANG L,YANG Y,WANG Y,et al. Astragalus membranaceus extract promotes neovascularisation by VEGF pathway in rat model of ischemic injury[J]. harmazie,2011,66(2):144-150.
[20] ZHANG Y I,HU G,LIN H C,et al. Radix astragali extract promotes angiogenesis involving vascular endothelial growth factor re-ceptor-related phosphatidylinositol 3-Kinase/Ak t-dependentpathway in human endothelial cells[J]. Phytother Res,2009,23(9):1205-1213.
[21] ZHANG L,YANG Y,WANG Y,et al. Astragalus membranaceus extract promotes neovascularisation by VEGF pathway in rat model of ischemic injury[J]. Harmazie,2011,66(2):144-150.
[22]苏丹,严浩然,张蓓蓓,等.黄芪对慢性心衰大鼠心功能及心肌肌浆网钙泵基因表达的影响[J].中药材杂志,2009,32(1):85-88.
[23]苏敬泽,林谦,农一兵.黄芪组分对乳鼠肥大心肌细胞ATP含量的影响[J].中国中医基础医学杂志,2011,17(5):500-502.
[24] XIA Y,JAVADOV S,GAN T X,et al. Distinct KATP channels mediate the antihypertrophic effects of adenosine receptor activation in neonatal rat ventricular myocytes[J]. J Pharmacol Exp Ther,2007,320(1):14-21.
[25]佟宇,王晶. mito KATP在黄芪甲苷抑制心肌细胞肥大中的作用[J].中药药理与临床,2013,29(3):51-54.
[26] CHEN P,XIE Y,SHEN E,et al. Astragaloside IV attenuates myocardial fibrosis by inhibiting TGF-β1 signaling in coxsackievirus B3-induced cardiomyopathy[J]. Eur J Pharmacol,2011,658(2/3):168-174.
[27]马兰,关振中.黄芪注射液对大鼠左室重构及凋亡基因Caspase-3的影响[J].中国中西医结合杂志,2005,25(7):646-649.
[28]韦育林.黄芪注射液对充血性心力衰竭患者细胞因子和血管紧张素Ⅱ的影响[J].中国临床康复杂志,2006,10(3):54-56.
[29]郑仕中,邹洪波,王天宇,等.黄芪对小鼠心肌β受体影响初探[J].南京医科大学学报,1997,17(1):78.
[30]石瑞如,刘艳红,何路明,等.黄芪对老年大鼠脑M受体、心肌β受体等的调节作用[J].中国中医药科技,1998,5(1):27-30.
[31]王奇玲.黄芪皂甙对离体工作心脏的肌力作用及其可能机制[J].中国中药杂志,1992,17(9):557.
[32]秦腊梅,牛福林,徐西,等.党参、黄芪补益心气作用的研究[J].中药药理与临床,1987,23(3):31-35.
[33] ARAI M,MATSUI H,PERIASAMY M. Sarcoplasmic Reticulum Gene Expression in Cardiac Hypertrophy and Heart Failure[J].Circ Res,1994,74(6):555-564.
[34] XU XL,JI H,GU SY,et al. Modification of alterations in cardiac function and sarcoglasmic reticulum by astragalosideⅣin myocardial injury in vivo[J]. Eur Pharmacol,2007,568(1-3):203-212.
[35]祝丹,陈启兰,祝光礼,等.复方黄芪养心合剂对缺血性心律失常大鼠心肌细胞缝隙连接蛋白Cx43磷酸化和去磷酸化的影响[J].中国生化药物杂志,2016,36(10):29-32.
[36]夏益.复方黄芪养心合剂对大鼠缺血性心律失常心肌细胞CX43在位点Ser262和Ser368磷酸化的影响[D].杭州:浙江中医药大学,2016.
[37]陆文娟,周婧,马宏跃,等.黄芪甲苷、人参总皂苷和西洋参总皂苷对蟾酥致小鼠心律失常的影响[J].南京中医药大学学报,2012,28(1):61-64.
[38]杨华伟.黄芪注射液治疗慢性心衰及预防洋地黄中毒的实验和临床研究[D].广州:广州中医药大学,2005.
[39]卢振,胡申江,孙坚,等.黄芪对SHR心肌SERCA表达及血管平滑肌钙离子水平影响的初步探讨[J].浙江中医学院学报,2004,28(5):60.
[40]刘燕,石海莲,马春来,等.黄芪甲苷对高血压大鼠血管肥厚及ET-1诱导离体血管收缩功能的影响[J].中国新药与临床杂志,2009,28(11):846-851.
[41]韩冬.黄芪甲苷对实验性糖尿病大鼠降糖、调脂和抗氧化作用的研究[J].现代中西医结合杂志,2016,25(4):360-364.
[42]张敬芳,杨雪琴,张学福.黄芪多糖抗动脉粥样硬化作用及其机制的研究[J].中国动脉硬化杂志,2011,19(3):268.
[2]温燕梅.黄芪的化学成分研究进展[J].中成药,2006,28(6):879-883.
[3]石刚刚,陈锦香,李长朝,等.黄芪注射液对冠状动脉直接作用的研究[J].中药新药与临床药理,1999,10(1):38-39.
[4]雷春利,陈羽,吕文伟,等.黄芪总皂苷对犬急性心肌梗塞缺血面积、心外膜电图、心肌酶的影响[J].白求恩医科大学学报,1995,21(2):111-113.
[5]闵清,白育庭,劳超,等.黄芪多糖对大鼠心肌缺血保护作用及其机制研究[J].时珍国医国药,2011,22(4):921-923.
[6]关凤英,李红,于秀霞,等.黄芪注射液预适应对大鼠实验性心肌缺血再灌注损伤的保护作用及其机制[J].中国老年学杂志,2010,30(21):3126-3129.
[7]周吉燕,樊懿,孔建龙,等.黄芪中不同提取成分对在体大鼠心肌缺血-再灌注损伤的心功能影响[J].中国中药杂志,2000,25(5):300.
[8]李铁成,张静峰,马宝丰,等.黄芪多糖对心肌缺血再灌注损伤的保护作用[J].天津中医药,2014,31(8):490-492.
[9]王媛媛,彭洋,张琦,等. ERK1/2信号通路对黄芪苷Ⅳ抗H2O2诱导H9c2细胞氧化损伤的作用[J].中国应用生理学杂志,2011,27(3):363-367.
[10]张琦,彭洋,刘艳霞,等.黄芪苷Ⅳ通过PI3K/Akt信号通路抗H2O2诱导的H9c2细胞氧化损伤[J].中草药,2010,41(6):955-959.
[11] HU J Y,HAN J,CHU Z G,et al. AstragalosideⅣattenuates hypoxia-induced cardiomyocyte damage in rats by upregulating superoxide dismutase-1 levels[J]. Clin Exp Pharmacol Physiol,2009,36(4):351-3571.
[12] JONES S P,TROCHA S D,STRANGE M B,et al. Leukocyte and endothelial cell adhesion molecules in a chronic murine model of myocardial reperfusion injury[J]. American Journal of Physiology,2000,279(5):2196-2201.
[13] LUO S F,FANG R Y,HSIEH H L,et al. Involvement of MAPKs and NF-KB in tumor necrosis factorα–induced vascular cell adhesion molecule 1 expression in human rheumatoid arthritis synovial fibroblasts[J]. Arthritis and Rheumatism,2010,62(1):105-116.
[14] MATSUMOTO T,TURESSON I,BOOK M,et al. p38 MAP kinase negatively regulates endothelial cell survival,proliferation,and differentiation in FGF-2-stimulated angiogenesis[J].Journal of Cell Biology,2002,156(1):149-160.
[15] HAI Y Z,YONG H G,ZHI Y W,et al. Astragalus Polysaccharide Suppresses the Expression of Adhesion Molecules through the Regulation of the p38 MAPK Signaling Pathway in Human Cardiac Microvascular Endothelial Cells after Ischemia-Reperfusion Injury[J]. Evid Based Complement Alternat Med,2013,66(4):280-293.
[16]茵涛,杨英珍,杨学义,等.黄芪对小鼠急性病毒性心肌炎作用的心肌细胞电生理研究[J].中国中西医结合杂志,1994,14(5):292-294.
[17]郭棋,彭天庆,杨英珍,等.黄芪对柯萨奇B_3病毒感染培养大鼠心肌细胞Ca2+内流及该病毒RNA复制[J].中国中西医结合杂志,1995,15(8):483-485.
[18] ZHANG Y I,HU G,LIN H C,et al. Radix astragali extract promotes angiogenesis involving vascular endothelial growth factor re-ceptor-related phosphatidylinositol 3-Kinase/Ak t-dependentpathway in human endothelial cells[J]. Phytother Res,2009,23(9):1205-1213.
[19] ZHANG L,YANG Y,WANG Y,et al. Astragalus membranaceus extract promotes neovascularisation by VEGF pathway in rat model of ischemic injury[J]. harmazie,2011,66(2):144-150.
[20] ZHANG Y I,HU G,LIN H C,et al. Radix astragali extract promotes angiogenesis involving vascular endothelial growth factor re-ceptor-related phosphatidylinositol 3-Kinase/Ak t-dependentpathway in human endothelial cells[J]. Phytother Res,2009,23(9):1205-1213.
[21] ZHANG L,YANG Y,WANG Y,et al. Astragalus membranaceus extract promotes neovascularisation by VEGF pathway in rat model of ischemic injury[J]. Harmazie,2011,66(2):144-150.
[22]苏丹,严浩然,张蓓蓓,等.黄芪对慢性心衰大鼠心功能及心肌肌浆网钙泵基因表达的影响[J].中药材杂志,2009,32(1):85-88.
[23]苏敬泽,林谦,农一兵.黄芪组分对乳鼠肥大心肌细胞ATP含量的影响[J].中国中医基础医学杂志,2011,17(5):500-502.
[24] XIA Y,JAVADOV S,GAN T X,et al. Distinct KATP channels mediate the antihypertrophic effects of adenosine receptor activation in neonatal rat ventricular myocytes[J]. J Pharmacol Exp Ther,2007,320(1):14-21.
[25]佟宇,王晶. mito KATP在黄芪甲苷抑制心肌细胞肥大中的作用[J].中药药理与临床,2013,29(3):51-54.
[26] CHEN P,XIE Y,SHEN E,et al. Astragaloside IV attenuates myocardial fibrosis by inhibiting TGF-β1 signaling in coxsackievirus B3-induced cardiomyopathy[J]. Eur J Pharmacol,2011,658(2/3):168-174.
[27]马兰,关振中.黄芪注射液对大鼠左室重构及凋亡基因Caspase-3的影响[J].中国中西医结合杂志,2005,25(7):646-649.
[28]韦育林.黄芪注射液对充血性心力衰竭患者细胞因子和血管紧张素Ⅱ的影响[J].中国临床康复杂志,2006,10(3):54-56.
[29]郑仕中,邹洪波,王天宇,等.黄芪对小鼠心肌β受体影响初探[J].南京医科大学学报,1997,17(1):78.
[30]石瑞如,刘艳红,何路明,等.黄芪对老年大鼠脑M受体、心肌β受体等的调节作用[J].中国中医药科技,1998,5(1):27-30.
[31]王奇玲.黄芪皂甙对离体工作心脏的肌力作用及其可能机制[J].中国中药杂志,1992,17(9):557.
[32]秦腊梅,牛福林,徐西,等.党参、黄芪补益心气作用的研究[J].中药药理与临床,1987,23(3):31-35.
[33] ARAI M,MATSUI H,PERIASAMY M. Sarcoplasmic Reticulum Gene Expression in Cardiac Hypertrophy and Heart Failure[J].Circ Res,1994,74(6):555-564.
[34] XU XL,JI H,GU SY,et al. Modification of alterations in cardiac function and sarcoglasmic reticulum by astragalosideⅣin myocardial injury in vivo[J]. Eur Pharmacol,2007,568(1-3):203-212.
[35]祝丹,陈启兰,祝光礼,等.复方黄芪养心合剂对缺血性心律失常大鼠心肌细胞缝隙连接蛋白Cx43磷酸化和去磷酸化的影响[J].中国生化药物杂志,2016,36(10):29-32.
[36]夏益.复方黄芪养心合剂对大鼠缺血性心律失常心肌细胞CX43在位点Ser262和Ser368磷酸化的影响[D].杭州:浙江中医药大学,2016.
[37]陆文娟,周婧,马宏跃,等.黄芪甲苷、人参总皂苷和西洋参总皂苷对蟾酥致小鼠心律失常的影响[J].南京中医药大学学报,2012,28(1):61-64.
[38]杨华伟.黄芪注射液治疗慢性心衰及预防洋地黄中毒的实验和临床研究[D].广州:广州中医药大学,2005.
[39]卢振,胡申江,孙坚,等.黄芪对SHR心肌SERCA表达及血管平滑肌钙离子水平影响的初步探讨[J].浙江中医学院学报,2004,28(5):60.
[40]刘燕,石海莲,马春来,等.黄芪甲苷对高血压大鼠血管肥厚及ET-1诱导离体血管收缩功能的影响[J].中国新药与临床杂志,2009,28(11):846-851.
[41]韩冬.黄芪甲苷对实验性糖尿病大鼠降糖、调脂和抗氧化作用的研究[J].现代中西医结合杂志,2016,25(4):360-364.
[42]张敬芳,杨雪琴,张学福.黄芪多糖抗动脉粥样硬化作用及其机制的研究[J].中国动脉硬化杂志,2011,19(3):268.