麦芪降糖丸对ob/ob小鼠血糖的影响
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摘要
目的研究麦芪降糖丸对ob/ob小鼠血糖的影响。方法 8周龄雄性ob/ob小鼠随机分成模型组,麦芪降糖丸高、中、低剂量(8 g/kg、4 g/kg、2 g/kg)组和二甲双胍(250 mg/kg)组,各给药组分别灌胃给予相应剂量的药物,每天1次,连续10周,同周龄正常对照组C57BL/6 J小鼠和模型对照组ob/ob小鼠给予等体积的溶剂。检测每组小鼠日平均进食量和体重、空腹血糖、血清胰岛素水平和胰腺病理变化。结果麦芪降糖丸给药2周、6周、8周、10周时能明显降低ob/ob小鼠空腹血糖(P <0. 05,P<0. 01),给药10周对小鼠日平均进食量和体重无明显影响,能减轻ob/ob小鼠的胰腺胰岛细胞的病变程度,提高ob/ob小鼠空腹血清胰岛素水平(P<0. 05)。结论麦芪降糖丸能降低ob/ob小鼠血糖,可能与减轻胰腺病理损伤,促进胰岛素分泌有关。
Objective To investigate the effects of a Chinese medicine,Mai Qi Jiang Tang pill,on the blood glucose level in ob/ob mice. Methods Eight-week old male ob/ob mice were randomly divided into Mai Qi Jiang Tang pill high( 8 g/kg),medium( 4 g/kg),and low( 2 g/kg) dose groups,as well as a metformin( 250 m g/kg) group,were orally administered drugs once daily for 10 weeks. Age-matched C57 BL/6 J control mice and ob/ob mice were orally administered an equal volume of vehicle. Average food consumption per day and body weights of the mice were recorded,and fasting blood glucose and insulin levels were measured using a glycemic instrument and enzyme-linked immunosorbent assay,respectively. Pathological changes of the pancreas were observed using hematoxylin and eosin staining. Results Compared with the control ob/ob mice,ob/ob mice administered Mai Qi Jiang Tang pill exhibited significantly decreased fasting blood glucose levels at 2,6,8,and 10 weeks after treatment( P< 0. 05,P < 0. 01). Mai Qi Jiang Tang pill also attenuated the degree of pathological changes observed in islet cells and increased serum insulin levels( P < 0. 05),but elicited no significant difference in body weight or average food consumption per day at 10 weeks after treatment.Conclusions Mai Qi Jiang Tang pill can decrease blood glucose levels of ob/ob mice,which may be related to the amelioration of pancreatic pathology and promotion of insulin secretion.
Objective To investigate the effects of a Chinese medicine,Mai Qi Jiang Tang pill,on the blood glucose level in ob/ob mice. Methods Eight-week old male ob/ob mice were randomly divided into Mai Qi Jiang Tang pill high( 8 g/kg),medium( 4 g/kg),and low( 2 g/kg) dose groups,as well as a metformin( 250 m g/kg) group,were orally administered drugs once daily for 10 weeks. Age-matched C57 BL/6 J control mice and ob/ob mice were orally administered an equal volume of vehicle. Average food consumption per day and body weights of the mice were recorded,and fasting blood glucose and insulin levels were measured using a glycemic instrument and enzyme-linked immunosorbent assay,respectively. Pathological changes of the pancreas were observed using hematoxylin and eosin staining. Results Compared with the control ob/ob mice,ob/ob mice administered Mai Qi Jiang Tang pill exhibited significantly decreased fasting blood glucose levels at 2,6,8,and 10 weeks after treatment( P< 0. 05,P < 0. 01). Mai Qi Jiang Tang pill also attenuated the degree of pathological changes observed in islet cells and increased serum insulin levels( P < 0. 05),but elicited no significant difference in body weight or average food consumption per day at 10 weeks after treatment.Conclusions Mai Qi Jiang Tang pill can decrease blood glucose levels of ob/ob mice,which may be related to the amelioration of pancreatic pathology and promotion of insulin secretion.
引文
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[7]张婧,刘长山,刘阳,等.黄芪对2型糖尿病大鼠胰岛素抵抗及肾脏损伤的影响[J].临床合理用药,2013,6(4):54-55.
[8]仝国辉,张懿,谭壮生.地黄寡糖对实验性高血糖小鼠糖脂代谢的影响[J].毒理学杂志,2011,25(2):117-119.
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[10]冯果,陈娟,刘文,等.天花粉有效成分及药理活性研究进展[J].微量元素与健康研究,2015,32(6):59-62.
[11]安丽萍,王英平,刘晓梅,等.五味子油对链脲佐菌素诱导的2型糖尿病大鼠的影响[J].中草药,2012,43,(3):552-556.
[12]金银萍,李珊珊,郑颖,等.五味子抗糖尿病和抗氧化作用研究进展[J].特产研究,2017,(1):64-68.
[13]孙雅文,张明发,沈雅琴.女贞子及其活性成分降血糖、调血脂、抗肥胖作用研究进展[J].药物评价研究,2016,39(6):1086-1091.
[14]胡云飞,徐国兵.牡丹皮及其主要成分丹皮酚的药理作用研究进展[J].安徽医药,2014,18(4):589.
[15]崔珏,李超,尤健,徐晓栋.白茅根多糖改善糖尿病小鼠糖脂代谢作用的研究[J].食品科学,2013,33(19):302-305.
[16] D’souza AM, Johnson JD, Clee SM, et al. Suppressing hyperinsulinemia prevents obesity but causes rapid onset of diabetes in leptin-deficient Lepob/obmice[J]. Mol Metab,2016,5(11):1103-1112.
[2] International Diabetes Federation. IDF Diabetes Atlas(8th Edition)[M]. Brussels, Belgium:International Diabetes Federation,2017.
[3]吕姗珊,唐桂英,周年华,等.麦芪降糖丸对2型糖尿病模型大鼠影响的实验研究[J].世界中西医结合杂志,2014,9(9):934-937.
[4]薛欣,李玉梅,陈冰,等.瘦素缺陷型ob/ob小鼠和C57BL/6J小鼠生物学特性的对比研究[J].中国医科大学学报,2015,44(11):983-986.
[5] Unger RH,Roth MG. A new biology of diabetes revealed by leptin[J].Cell Metab,2015,21(1):15-20.
[6]张璐欣,周学谦,李德坤,等.麦冬多糖的化学组成、分析方法和药理作用研究进展[J].药物评价研究,2017,40(2):279-284.
[7]张婧,刘长山,刘阳,等.黄芪对2型糖尿病大鼠胰岛素抵抗及肾脏损伤的影响[J].临床合理用药,2013,6(4):54-55.
[8]仝国辉,张懿,谭壮生.地黄寡糖对实验性高血糖小鼠糖脂代谢的影响[J].毒理学杂志,2011,25(2):117-119.
[9]傅盼盼,洪铁,杨振.党参多糖对糖尿病小鼠胰岛素抵抗的改善作用[J].时珍国医国药,2008,19(10):2414-2416.
[10]冯果,陈娟,刘文,等.天花粉有效成分及药理活性研究进展[J].微量元素与健康研究,2015,32(6):59-62.
[11]安丽萍,王英平,刘晓梅,等.五味子油对链脲佐菌素诱导的2型糖尿病大鼠的影响[J].中草药,2012,43,(3):552-556.
[12]金银萍,李珊珊,郑颖,等.五味子抗糖尿病和抗氧化作用研究进展[J].特产研究,2017,(1):64-68.
[13]孙雅文,张明发,沈雅琴.女贞子及其活性成分降血糖、调血脂、抗肥胖作用研究进展[J].药物评价研究,2016,39(6):1086-1091.
[14]胡云飞,徐国兵.牡丹皮及其主要成分丹皮酚的药理作用研究进展[J].安徽医药,2014,18(4):589.
[15]崔珏,李超,尤健,徐晓栋.白茅根多糖改善糖尿病小鼠糖脂代谢作用的研究[J].食品科学,2013,33(19):302-305.
[16] D’souza AM, Johnson JD, Clee SM, et al. Suppressing hyperinsulinemia prevents obesity but causes rapid onset of diabetes in leptin-deficient Lepob/obmice[J]. Mol Metab,2016,5(11):1103-1112.