摘要
目的研究麦芪降糖丸对ob/ob小鼠血糖的影响。方法 8周龄雄性ob/ob小鼠随机分成模型组,麦芪降糖丸高、中、低剂量(8 g/kg、4 g/kg、2 g/kg)组和二甲双胍(250 mg/kg)组,各给药组分别灌胃给予相应剂量的药物,每天1次,连续10周,同周龄正常对照组C57BL/6 J小鼠和模型对照组ob/ob小鼠给予等体积的溶剂。检测每组小鼠日平均进食量和体重、空腹血糖、血清胰岛素水平和胰腺病理变化。结果麦芪降糖丸给药2周、6周、8周、10周时能明显降低ob/ob小鼠空腹血糖(P <0. 05,P<0. 01),给药10周对小鼠日平均进食量和体重无明显影响,能减轻ob/ob小鼠的胰腺胰岛细胞的病变程度,提高ob/ob小鼠空腹血清胰岛素水平(P<0. 05)。结论麦芪降糖丸能降低ob/ob小鼠血糖,可能与减轻胰腺病理损伤,促进胰岛素分泌有关。
Objective To investigate the effects of a Chinese medicine,Mai Qi Jiang Tang pill,on the blood glucose level in ob/ob mice. Methods Eight-week old male ob/ob mice were randomly divided into Mai Qi Jiang Tang pill high( 8 g/kg),medium( 4 g/kg),and low( 2 g/kg) dose groups,as well as a metformin( 250 m g/kg) group,were orally administered drugs once daily for 10 weeks. Age-matched C57 BL/6 J control mice and ob/ob mice were orally administered an equal volume of vehicle. Average food consumption per day and body weights of the mice were recorded,and fasting blood glucose and insulin levels were measured using a glycemic instrument and enzyme-linked immunosorbent assay,respectively. Pathological changes of the pancreas were observed using hematoxylin and eosin staining. Results Compared with the control ob/ob mice,ob/ob mice administered Mai Qi Jiang Tang pill exhibited significantly decreased fasting blood glucose levels at 2,6,8,and 10 weeks after treatment( P< 0. 05,P < 0. 01). Mai Qi Jiang Tang pill also attenuated the degree of pathological changes observed in islet cells and increased serum insulin levels( P < 0. 05),but elicited no significant difference in body weight or average food consumption per day at 10 weeks after treatment.Conclusions Mai Qi Jiang Tang pill can decrease blood glucose levels of ob/ob mice,which may be related to the amelioration of pancreatic pathology and promotion of insulin secretion.
引文
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