七参连湿疹膏对特应性皮炎模型豚鼠的改善作用研究
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摘要
目的:考察七参连湿疹膏对特应性皮炎(AD)模型豚鼠的改善作用,并探讨其可能机制。方法:将雌性豚鼠随机分为正常对照组,模型对照组,基质对照组(七参连湿疹膏基质,1 g/kg),七参连湿疹膏低、中、高剂量组(0.5、1、2 g/kg)和丁酸氢化可的松乳膏组(0.5 g/kg),每组10只。以卵清蛋白为抗原,激发豚鼠皮肤变态反应以复制AD模型。末次激发48 h后,正常对照组和模型对照组豚鼠涂以生理盐水,基质对照组豚鼠涂以七参连湿疹膏基质,各给药组豚鼠涂以相应药物;每天2次,连续14 d。记录各组豚鼠给药前后的红斑、水肿、抓痕等症状评分;采用苏木精-伊红染色法观察豚鼠皮肤组织形态学特征;采用TUNEL法检测豚鼠皮肤组织角质形成细胞凋亡情况;采用酶联免疫吸附测定法检测豚鼠皮肤组织干扰素γ(IFN-γ)水平。结果:与正常对照组比较,模型对照组和基质对照组豚鼠皮肤组织中均可见炎症细胞浸润、表皮角化过度、棘层增厚等症状,其红斑、水肿、抓痕评分及总评分和皮肤组织中IFN-γ水平均显著升高,角质形成细胞凋亡数均显著减少(P<0.01)。与模型对照组和基质对照组比较,各给药组豚鼠上述症状均有不同程度的减轻,其中各给药组豚鼠给药后的红斑、水肿、抓痕评分及总评分以及七参连湿疹膏中、高剂量组和丁酸氢化可的松乳膏组豚鼠皮肤组织中IFN-γ水平均显著降低,且给药组上述评分均显著低于同组给药前(P<0.05或P<0.01);各给药组豚鼠角质形成细胞凋亡数均显著增加(P<0.05或P<0.01)。结论:七参连湿疹膏对AD模型豚鼠具有一定的改善作用,这种作用可能与促进角质形成细胞凋亡、降低皮肤组织INF-γ水平有关。
OBJECTIVE:To investigate the improvement effects of Qishenlian eczema cream on the atopic dermatitis(AD)model guinea pigs,and to investigate its possible mechanism. METHODS:Female guinea pigs were randomly divided into normal control group,model control group,matrix control group(Qishenlian eczema cream matrix,1 g/kg),Qishenlian eczema cream low-dose,medium-dose and high-dose groups(0.5,1,2 g/kg) and Hydrocortisone butyrate cream group(0.5 g/kg),with 10 guinea pigs in each group. Using ovalbumin as antigen,the skin allergy of guinea pigs was stimulated to induce AD model.Forty-eight hours after the last excitation,guinea pigs were smeared with normal saline in normal control group and model control group,while those were smeared with Qishenlian eczema cream matrix in matrix control group,and administration groups were treated with relevant medicine,twice a day,for consecutive 14 d. The scores of erythema,edema and scratch before and after administration were recorded in each group. The morphological characteristics of skin were observed by HE staining. TUNEL method was used to detect the apoptosis of keratinocytes in skin tissue. The level of IFN-γ in skin lesions was detected by ELISA.RESULTS:Compared with normal control group,inflammatory cell infiltration,hyperkeratosis of epidermis and thickening of spinous layer were observed in skin tissue of guinea pigs in model control group and matrix control group;the scores of erythema,edema and scratch and their total score,the level of IFN-γ in skin tissue were increased significantly;the apoptosis number of keratinocytes was decreased significantly(P<0.01). Compared with model control group and matrix control group, above symptoms of medication groups were relieved to different extents;the scores of erythema,edema and scratch and their total score after medication in medication groups,the level of IFN-γ in skin tissue in Qishenlian eczema cream medium-dose and high-dose groups and Hydrocortisone butyrate cream group were decreased significantly, and above scores in medication groups were significantly lower than before medication(P<0.05 or P<0.01);the apoptosis number of keratinocytes was increased significantly in medication groups(P<0.05 or P<0.01). CONCLUSIONS:Qishenlian eczema cream has certain improvement effect on AD model guinea pigs, the mechanism of which may be associated with promoting the keratinocyte apoptosis,reducing the level of INF-γ in skin tissue.
OBJECTIVE:To investigate the improvement effects of Qishenlian eczema cream on the atopic dermatitis(AD)model guinea pigs,and to investigate its possible mechanism. METHODS:Female guinea pigs were randomly divided into normal control group,model control group,matrix control group(Qishenlian eczema cream matrix,1 g/kg),Qishenlian eczema cream low-dose,medium-dose and high-dose groups(0.5,1,2 g/kg) and Hydrocortisone butyrate cream group(0.5 g/kg),with 10 guinea pigs in each group. Using ovalbumin as antigen,the skin allergy of guinea pigs was stimulated to induce AD model.Forty-eight hours after the last excitation,guinea pigs were smeared with normal saline in normal control group and model control group,while those were smeared with Qishenlian eczema cream matrix in matrix control group,and administration groups were treated with relevant medicine,twice a day,for consecutive 14 d. The scores of erythema,edema and scratch before and after administration were recorded in each group. The morphological characteristics of skin were observed by HE staining. TUNEL method was used to detect the apoptosis of keratinocytes in skin tissue. The level of IFN-γ in skin lesions was detected by ELISA.RESULTS:Compared with normal control group,inflammatory cell infiltration,hyperkeratosis of epidermis and thickening of spinous layer were observed in skin tissue of guinea pigs in model control group and matrix control group;the scores of erythema,edema and scratch and their total score,the level of IFN-γ in skin tissue were increased significantly;the apoptosis number of keratinocytes was decreased significantly(P<0.01). Compared with model control group and matrix control group, above symptoms of medication groups were relieved to different extents;the scores of erythema,edema and scratch and their total score after medication in medication groups,the level of IFN-γ in skin tissue in Qishenlian eczema cream medium-dose and high-dose groups and Hydrocortisone butyrate cream group were decreased significantly, and above scores in medication groups were significantly lower than before medication(P<0.05 or P<0.01);the apoptosis number of keratinocytes was increased significantly in medication groups(P<0.05 or P<0.01). CONCLUSIONS:Qishenlian eczema cream has certain improvement effect on AD model guinea pigs, the mechanism of which may be associated with promoting the keratinocyte apoptosis,reducing the level of INF-γ in skin tissue.
引文
[1]杜宇,杨西群,熊霞,等.复方氟米松乳膏联合复方利多卡因乳膏治疗慢性湿疹疗效观察[J].泸州医学院学报,2010,33(5):520-522.
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[3]BRUNNER PM,GUTTMAN-YASSKY E,LEUNG DY.The immunology of atopic dermatitis and its reversibility with broad spectrum and targeted therapies[J].J Allergy Clin Immunol,2017,139(4S):S65-S67.
[4]JURAKI?TON?I?R,MARINOVI?B.The role of impaired epidermal barrier function in atopic dermatitis[J].Acta Dermatovenerol Croat,2016,24(2):95-109.
[5]中华医学会皮肤性病学分会免疫学组,特应性皮炎协作研究中心.中国特应性皮炎诊疗指南:2014版[J].全科医学临床与教育,2014,12(6):603-606.
[6]蒋祖玲,汤倩倩,黄安,等.慢性湿疹的药物外治治疗研究进展[J].医学综述,2018,24(20):4103-4107.
[7]王涛,刘跃华,尹佳,等.奥洛他定与西替利嗪治疗皮肤瘙痒症的有效性和安全性对照研究[J].临床皮肤科杂志,2011,40(5):300-302.
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[9]安军艳,王利兰,姜红伶.浅谈祛风、清热、除湿法在常见皮肤病中的重要应用[J].世界最新医学信息文摘,2018,18(96):317-318.
[10]尚静雯.七参连软膏治疗皮炎湿疹43例临床疗效观察[J].中国皮肤性病学杂志,2007,21(7):448.
[11]梁玲,相茂芹.七参连软膏治疗皮炎湿疹疗效观察[J].中国麻风皮肤病杂志,2006,22(12):1045-1046.
[12]易雪梅,温海,付文成,等.复方酮康唑喷膜对豚鼠湿疹模型的实验研究[J].中国皮肤性病学杂志,2005,19(3):141-148.
[13]SHIOHARA T,HAYAKAWA J,MIZUKAWA Y.Animal models for atopic dermatitis:are they relevant to human disease?[J].J Dermatol Sci,2004,36(1):1-9.
[14]胡佳,张美华,毕志刚,等.卵清蛋白经皮致敏诱发BALB/c小鼠特应性皮炎样病变的探讨[J].临床皮肤科杂志,2006,35(6):373-375.
[15]刘欣欣,陈立新,王莹,等.特异性免疫治疗对BALB/c小鼠特应性皮炎模型的疗效观察[J].中国皮肤性病学杂志,2016,30(11):1112-1115.
[16]何康.维A酸霜与丁酸氢化可的松软骨联合治疗慢性湿疹疗效观察[J].广西医学,2008,26(9):1373.
[17]刘琪琪.尤卓尔软膏治疗湿疹及神经性皮炎临床研究[J].国外医药抗生素分册,2015,36(5):231-233.
[18]孙占学,张丰川,李元文.甘石青黛膏对大鼠湿疹模型皮损角质形成细胞与淋巴细胞凋亡的影响[J].中华临床医师杂志,2013,7(3):1146-1150.
[19]祝青,刁庆春,白晋,等.聚焦超声对慢性湿疹模型豚鼠皮肤淋巴细胞和角质形成细胞凋亡的影响[J].中国医学影像技术,2008,24(2):167-170.
[20]陆丽明,程楷涛.湿疹发生机理的研究进展[J].亚太传统医药,2007,3(9):76-78.
[21]瞿旻晔,袁晓琳,马健.特应性皮炎发病过程及其机制研究现状探讨[J].中国皮肤性病学杂志,2015,29(10):1085-1087.
[22]PENG W,NOVAK N.Pathogenesis of atopic dermatitis[J].Clin Exp Allergy,2015,45(3):566-574.
[23]盛楠,宗文凯,余美文,等.咪唑斯汀治疗湿疹和特应性皮炎临床疗效及免疫机制的初步研究[J].临床皮肤科杂志,2009,38(7):440-442.
[2]袁伟,晏文,陈晓红,等.复方甘草酸苷联合氟米松乳膏治疗慢性湿疹疗效观察[J].中国皮肤性病学杂志,2009,23(9):612-613.
[3]BRUNNER PM,GUTTMAN-YASSKY E,LEUNG DY.The immunology of atopic dermatitis and its reversibility with broad spectrum and targeted therapies[J].J Allergy Clin Immunol,2017,139(4S):S65-S67.
[4]JURAKI?TON?I?R,MARINOVI?B.The role of impaired epidermal barrier function in atopic dermatitis[J].Acta Dermatovenerol Croat,2016,24(2):95-109.
[5]中华医学会皮肤性病学分会免疫学组,特应性皮炎协作研究中心.中国特应性皮炎诊疗指南:2014版[J].全科医学临床与教育,2014,12(6):603-606.
[6]蒋祖玲,汤倩倩,黄安,等.慢性湿疹的药物外治治疗研究进展[J].医学综述,2018,24(20):4103-4107.
[7]王涛,刘跃华,尹佳,等.奥洛他定与西替利嗪治疗皮肤瘙痒症的有效性和安全性对照研究[J].临床皮肤科杂志,2011,40(5):300-302.
[8]李曰庆,何清湖.中医外科学[M].9版.北京:中国中医药出版社,2012:142-147.
[9]安军艳,王利兰,姜红伶.浅谈祛风、清热、除湿法在常见皮肤病中的重要应用[J].世界最新医学信息文摘,2018,18(96):317-318.
[10]尚静雯.七参连软膏治疗皮炎湿疹43例临床疗效观察[J].中国皮肤性病学杂志,2007,21(7):448.
[11]梁玲,相茂芹.七参连软膏治疗皮炎湿疹疗效观察[J].中国麻风皮肤病杂志,2006,22(12):1045-1046.
[12]易雪梅,温海,付文成,等.复方酮康唑喷膜对豚鼠湿疹模型的实验研究[J].中国皮肤性病学杂志,2005,19(3):141-148.
[13]SHIOHARA T,HAYAKAWA J,MIZUKAWA Y.Animal models for atopic dermatitis:are they relevant to human disease?[J].J Dermatol Sci,2004,36(1):1-9.
[14]胡佳,张美华,毕志刚,等.卵清蛋白经皮致敏诱发BALB/c小鼠特应性皮炎样病变的探讨[J].临床皮肤科杂志,2006,35(6):373-375.
[15]刘欣欣,陈立新,王莹,等.特异性免疫治疗对BALB/c小鼠特应性皮炎模型的疗效观察[J].中国皮肤性病学杂志,2016,30(11):1112-1115.
[16]何康.维A酸霜与丁酸氢化可的松软骨联合治疗慢性湿疹疗效观察[J].广西医学,2008,26(9):1373.
[17]刘琪琪.尤卓尔软膏治疗湿疹及神经性皮炎临床研究[J].国外医药抗生素分册,2015,36(5):231-233.
[18]孙占学,张丰川,李元文.甘石青黛膏对大鼠湿疹模型皮损角质形成细胞与淋巴细胞凋亡的影响[J].中华临床医师杂志,2013,7(3):1146-1150.
[19]祝青,刁庆春,白晋,等.聚焦超声对慢性湿疹模型豚鼠皮肤淋巴细胞和角质形成细胞凋亡的影响[J].中国医学影像技术,2008,24(2):167-170.
[20]陆丽明,程楷涛.湿疹发生机理的研究进展[J].亚太传统医药,2007,3(9):76-78.
[21]瞿旻晔,袁晓琳,马健.特应性皮炎发病过程及其机制研究现状探讨[J].中国皮肤性病学杂志,2015,29(10):1085-1087.
[22]PENG W,NOVAK N.Pathogenesis of atopic dermatitis[J].Clin Exp Allergy,2015,45(3):566-574.
[23]盛楠,宗文凯,余美文,等.咪唑斯汀治疗湿疹和特应性皮炎临床疗效及免疫机制的初步研究[J].临床皮肤科杂志,2009,38(7):440-442.