4种肾阳虚模型的评价
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摘要
目的对4种肾阳虚模型进行比较,为模型选择提供依据。方法 40只健康雄性大鼠,随机分为空白组、氢化可的松组、腺嘌呤组、他巴唑组、氢化可的松联合腺嘌呤组,通过腿部肌肉注射氢化可的松(25 mg/kg)、灌胃腺嘌呤(200 mg/kg)、灌胃他巴唑(每周灌胃30 mg/kg)、腿部肌肉注射氢化可的松联合灌胃腺嘌呤(25 mg/kg,150 mg/kg)造模21 d,检测各组大鼠体质量、抓力、体温、脏器系数、骨骼强度变化情况评价肾阳虚模型。结果造模21 d后,除空白组外,各组大鼠均出现体质量减轻、抓力下降、体温下降、脏器系数改变以及骨骼强度下降,氢化可的松联合腺嘌呤组症状最为明显,腿部肌肉注射氢化可的松组大鼠症状较明显。结论腿部肌肉注射氢化可的松为较理想的模型,氢化可的松联合腺嘌呤的造模方式初步可行,有待继续探究。
Objective Comparison of four models of kidney yang deficiency, provide a basis for model selection.Methods 40 healthy male rats were randomly divided into blank group, hydrocortisone group, adenine group,tab azole group, hydrocortisone combined with adenine group, leg muscle injection of hydrocortisone(25 mg/kg),gavage adenine(200 mg/kg),gavage tazobactam(30 mg/kg),leg muscle injection of hydrocortisone(25 mg/kg)combined with gavage adenine(150 mg/kg) for 21 days, determination the changes of body weight, grip strength,body temperature, organ coefficient and bone strength in each group to evaluate the model of kidney yang deficiency.Results After 21 days of modeling,in addition to the blank group,each group of rats showed weight loss, grip strength decline,hypothermia,organ coefficient change,and decrease of bone strength,among them,hydrocortisone combined with adenine group showed the most obvious symptoms, while leg muscles injection of hydrocortisone showed more obvious symptoms in rats. Conclusion The model of leg muscle injection of hydrocortisone is an ideal single modeling method, the modeling method of hydrocortisone combined with adenine is preliminarily feasible, and needs to be further explored.
Objective Comparison of four models of kidney yang deficiency, provide a basis for model selection.Methods 40 healthy male rats were randomly divided into blank group, hydrocortisone group, adenine group,tab azole group, hydrocortisone combined with adenine group, leg muscle injection of hydrocortisone(25 mg/kg),gavage adenine(200 mg/kg),gavage tazobactam(30 mg/kg),leg muscle injection of hydrocortisone(25 mg/kg)combined with gavage adenine(150 mg/kg) for 21 days, determination the changes of body weight, grip strength,body temperature, organ coefficient and bone strength in each group to evaluate the model of kidney yang deficiency.Results After 21 days of modeling,in addition to the blank group,each group of rats showed weight loss, grip strength decline,hypothermia,organ coefficient change,and decrease of bone strength,among them,hydrocortisone combined with adenine group showed the most obvious symptoms, while leg muscles injection of hydrocortisone showed more obvious symptoms in rats. Conclusion The model of leg muscle injection of hydrocortisone is an ideal single modeling method, the modeling method of hydrocortisone combined with adenine is preliminarily feasible, and needs to be further explored.
引文
[1]严石林,高锋,吴斌,等.肾阳虚证半定量化操作标准的研究[J].现代中西医结合杂志, 2004, 13(6):701-702.
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[8]范润勇,王琳,黄勤挽,等.中药炮天雄对腺嘌呤致肾阳虚模型大鼠的治疗作用研究[J].亚太传统医药, 2017,14(6):16-20.
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[14]张子怡,陈宝军,张庆,等.肾阳虚证动物模型的造模方法与评价指标[J].福建中医药, 2014, 45(2):61-63.
[15]陶秀梅.“肾阳虚”模型及证候的代谢组学研究[D].上海:上海交通大学, 2009.
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[17]邹海淼,辛雪,孙伟,等.肾阳虚生化指标的现代研究进展[J].现代生物医学进展, 2015, 15(30):5989-5993.
[18]李晴晴,潘智敏.原发性骨质疏松症中医病变机制的研究进展[J].浙江中医杂志, 2010, 45(2):147.
[19]陈剑磨,张胜军,夏炳江.骨质疏松肾阳虚病证结合模型构建的实验研究[J].中国中医急症, 2015, 24(2):201-203.
[20]王拥军,卞琴,崔学军.“肾主骨”理论研究的思路与方法[J].上海中医药大学学报, 2010, 24(1):8-12.
[2]孙广仁.《内经》中阳虚的概念及相关的几个问题[J].山东中医药大学学报, 2005, 29(4):300-301.
[3]王根发,董竞成,段小红.中医肾之概念暨补肾理法方药及应用[J].中华中医药杂志, 2012, 27(12):3112-3115.
[4]杜江,李楠,王和鸣.肾虚模型造模方法及相关指标[J].中国组织工程研究与临床康复, 2010, 14(50):9433-9436.
[5]苟小军,韩宝侠,王朝廷,等.肾阳虚证造模方法考察[J].吉林中医药, 2009, 29(9):814-815.
[6]戴冰,张嘉妮,杨梦琳,等.氢化可的松致肾虚证小鼠模型的建立及相关指标的评价[J].中国实验动物学报, 2017,25(1):70-73.
[7]马晓宇,姜梦如,张涛,等.氢化可的松诱导大鼠肾阳虚模型评价[J].中国兽医学报, 2017, 37(9):1763-1765.
[8]范润勇,王琳,黄勤挽,等.中药炮天雄对腺嘌呤致肾阳虚模型大鼠的治疗作用研究[J].亚太传统医药, 2017,14(6):16-20.
[9]陈红淑,杨元宵,戴世杰,等.肾气丸对肾阳虚证大鼠肾间质纤维化的作用研究[J].中华中医药杂志, 2017,32(6):2700-2702.
[10]欧阳轶强,邹移海,张薇,等.对大鼠他巴唑肾阳虚证动物模型下丘脑-垂体-靶腺轴几项指标的考察[J].中华中医药学刊, 2006, 24(9):1658-1661.
[11]宋洁,李震,于海芳.从下丘脑-垂体-甲状腺轴研究肾阳虚证的现状与思考[J].时珍国医国药, 2009, 20(7):1808-1810.
[12]李蕊,刘曼丽,田心,等.不同剂量腺嘌呤诱导肾阳虚不育大鼠模型实验研[J].安徽中医学院学报, 2010, 29(2):41-44.
[13]秦文艳,陈贺,朱竟赫,等.不同氢化可的松小鼠肾阳虚模型制备方法对比研究[J].实验动物科学, 2017, 34(4):11-14.
[14]张子怡,陈宝军,张庆,等.肾阳虚证动物模型的造模方法与评价指标[J].福建中医药, 2014, 45(2):61-63.
[15]陶秀梅.“肾阳虚”模型及证候的代谢组学研究[D].上海:上海交通大学, 2009.
[16]林海雄,王晓彤,马延怀,等.三种肾阳虚建模方法对肾脏的影响[J].时珍国医国药, 2016, 27(4):1015-1017.
[17]邹海淼,辛雪,孙伟,等.肾阳虚生化指标的现代研究进展[J].现代生物医学进展, 2015, 15(30):5989-5993.
[18]李晴晴,潘智敏.原发性骨质疏松症中医病变机制的研究进展[J].浙江中医杂志, 2010, 45(2):147.
[19]陈剑磨,张胜军,夏炳江.骨质疏松肾阳虚病证结合模型构建的实验研究[J].中国中医急症, 2015, 24(2):201-203.
[20]王拥军,卞琴,崔学军.“肾主骨”理论研究的思路与方法[J].上海中医药大学学报, 2010, 24(1):8-12.