HPLC法同时测定益气复脉方中3种活性成分的含量
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摘要
目的建立同时测定益气复脉方中芍药苷、甘草苷和甘草酸含量的方法。方法采用HPLC法测定芍药苷、甘草苷和甘草酸的含量,Purospher RP C_(18)色谱柱(250mm×4.6mm,5μm);乙腈-1mL·L~(-1)磷酸进行梯度洗脱;流速为1.0mL·min~(-1);检测波长为230和237nm;柱温为30℃;进样量为10μL。结果芍药苷、甘草苷和甘草酸的质量浓度分别在0.02~0.31,0.02~0.32和0.02~0.30mg·mL~(-1)范围内线性关系良好,r值均为0.999 9,3种成分的平均回收率分别为97.98%,98.23%和97.49%,RSD值均小于3.00%。结论该方法简便、准确、重复性好,可用于益气复脉方的内在质量控制。
Objective To establish a method for the simultaneous determination of paeoniflorin,liquiritin and glycyrrhizic acid in Yiqifumai Prescription.Methods The content of paeoniflorin,liquiritin and glycyrrhizic acid were determined by HPLC method with a Purospher RP C_(18)column(250mm×4.6mm,5μm)and a mobile phase of acetonitrile-1mL·L~(-1)phosphoric acid solution for gradient elution.The flow rate was 1.0mL·min~(-1) and the detection wavelength was 230 and 237nm.The column temperature was 30℃ and the injection volume was 10μL.Results Paeoniflorin showed a good linear relationship from 0.02 to 0.31 mg·mL~(-1),liquiritin showed a good linear relationship from 0.02 to 0.32mg·mL~(-1),and glycyrrhizic acid showed a good linear relationship from 0.02 to 0.30 mg·mL~(-1),r values were 0.999 9.The average recoveries of the 3components were 97.98%,98.23%and97.49%,respectively,and RSD was less than 3.00%.Conclusion The method is simple,repeatable and accurate.It can be applied to the intrinsic quality control of Yiqifumai Prescription.
Objective To establish a method for the simultaneous determination of paeoniflorin,liquiritin and glycyrrhizic acid in Yiqifumai Prescription.Methods The content of paeoniflorin,liquiritin and glycyrrhizic acid were determined by HPLC method with a Purospher RP C_(18)column(250mm×4.6mm,5μm)and a mobile phase of acetonitrile-1mL·L~(-1)phosphoric acid solution for gradient elution.The flow rate was 1.0mL·min~(-1) and the detection wavelength was 230 and 237nm.The column temperature was 30℃ and the injection volume was 10μL.Results Paeoniflorin showed a good linear relationship from 0.02 to 0.31 mg·mL~(-1),liquiritin showed a good linear relationship from 0.02 to 0.32mg·mL~(-1),and glycyrrhizic acid showed a good linear relationship from 0.02 to 0.30 mg·mL~(-1),r values were 0.999 9.The average recoveries of the 3components were 97.98%,98.23%and97.49%,respectively,and RSD was less than 3.00%.Conclusion The method is simple,repeatable and accurate.It can be applied to the intrinsic quality control of Yiqifumai Prescription.
引文
[1]郭文达.益气复脉方治疗室性期前收缩的临床研究[D].北京:北京中医药大学,2015:47-49.
[2]李昊娲.益气复脉合剂治疗室性期前收缩作用机制的实验研究[D].北京:北京中医药大学,2013:39-73.
[3]陆小华,马骁,王建,等.赤芍的化学成分和药理作用研究进展[J].中草药,2015,46(4):595-602.
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[5]饶梦琳,唐蜜,何锦悦,等.芍药苷对大鼠局灶性脑缺血再灌注脑血流量及PGI2/TXA2平衡的影响[J].药学学报,2014,49(1):55-60.
[6]刘卉,单进军,康安,等.甘草酸和甘草次酸对芍药苷在大鼠体内药动学参数的影响[J].中草药,2013,44(12):1610-1614.
[7]郭春燕,罗强,孙黎,等.芍药苷对H2O2诱导SH-SY5Y神经细胞损伤的保护作用[J].中草药,2013,44(20):2864-2871.
[8]朱有胜,褚俊.甘草苷对大鼠动脉粥样硬化的作用及机制[J].广东医学,2015,36(3):365-368.
[9]奥·乌力吉,王秀兰,代那音台,等.HPLC法同时测定呼伦贝尔产赤芍防治心血管系统疾病有效物质群中3种成分的含量[J].中国药房,2014,25(39):3676-3678.
[10]刘玥.血小板骨架蛋白Gelsolin在冠心病血瘀证中的作用及赤芍川芎有效组分的干预效应研究[D].北京:中国中医科学院,2012:3-5.
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[13]王翠霞,赵立杰,尉西岗,等.炙甘草汤对糖尿病性心肌病心律失常发生率和心率变异性及QT离散度的影响[J].河北医药,2015,37(2):221-223.
[14]张明发,沈雅琴.甘草抗动脉粥样硬化和抗血栓形成研究进展[J].西北药学杂志,2011,26(3):222-226.
[15]朱广伟,张贵君,孙奕,等.醋白芍、炙甘草及其配伍载体中药效组分变化规律分析[J].世界中医药,2016,11(1):143-146,150.
[16]浦锦宝,郑军献,梁卫青,等.白芍提取物中芍药苷及总皂苷的含量测定[J].中国中医药科技,2011,18(4):326-327.
[17]朴春梅,梁会,曹佩雪,等.不同工艺制备的升麻葛根汤中七种成分的含量测定[J].中国现代医学杂志,2013,23(16):6-10.
[18]高鸿彬,傅芃,王晓君,等.HPLC法测定养阴清肺口服液中芍药苷的含量[J].西北药学杂志,2016,31(3):254-256.
[19]李鑫,张秀丽.高效液相色谱法同时测定人参养荣丸中芍药苷、阿魏酸和橙皮苷含量[J].中国药业,2016,25(17):68-70.
[20]国家药典委员会.中国药典:2015年版:一部[S].北京:中国医药科技出版社,2015:105-106.
[21]张士勇.芪丹芍龙合剂中芍药苷含量测定方法的研究[J].中医药临床杂志,2012,24(1):71-72.
[2]李昊娲.益气复脉合剂治疗室性期前收缩作用机制的实验研究[D].北京:北京中医药大学,2013:39-73.
[3]陆小华,马骁,王建,等.赤芍的化学成分和药理作用研究进展[J].中草药,2015,46(4):595-602.
[4]张玉龙,王梦月,杨静玉,等.炙甘草化学成分及药理作用研究进展[J].上海中医药大学学报,2015,29(3):99-102.
[5]饶梦琳,唐蜜,何锦悦,等.芍药苷对大鼠局灶性脑缺血再灌注脑血流量及PGI2/TXA2平衡的影响[J].药学学报,2014,49(1):55-60.
[6]刘卉,单进军,康安,等.甘草酸和甘草次酸对芍药苷在大鼠体内药动学参数的影响[J].中草药,2013,44(12):1610-1614.
[7]郭春燕,罗强,孙黎,等.芍药苷对H2O2诱导SH-SY5Y神经细胞损伤的保护作用[J].中草药,2013,44(20):2864-2871.
[8]朱有胜,褚俊.甘草苷对大鼠动脉粥样硬化的作用及机制[J].广东医学,2015,36(3):365-368.
[9]奥·乌力吉,王秀兰,代那音台,等.HPLC法同时测定呼伦贝尔产赤芍防治心血管系统疾病有效物质群中3种成分的含量[J].中国药房,2014,25(39):3676-3678.
[10]刘玥.血小板骨架蛋白Gelsolin在冠心病血瘀证中的作用及赤芍川芎有效组分的干预效应研究[D].北京:中国中医科学院,2012:3-5.
[11]郑世存,李晓宇,欧阳兵,等.芍药苷药理作用研究新进展[J].中国药物警戒,2012,9(2):100-103.
[12]郭芳序.炙甘草汤治疗心律失常的证治规律研究[D].北京:北京中医药大学,2011:36-38.
[13]王翠霞,赵立杰,尉西岗,等.炙甘草汤对糖尿病性心肌病心律失常发生率和心率变异性及QT离散度的影响[J].河北医药,2015,37(2):221-223.
[14]张明发,沈雅琴.甘草抗动脉粥样硬化和抗血栓形成研究进展[J].西北药学杂志,2011,26(3):222-226.
[15]朱广伟,张贵君,孙奕,等.醋白芍、炙甘草及其配伍载体中药效组分变化规律分析[J].世界中医药,2016,11(1):143-146,150.
[16]浦锦宝,郑军献,梁卫青,等.白芍提取物中芍药苷及总皂苷的含量测定[J].中国中医药科技,2011,18(4):326-327.
[17]朴春梅,梁会,曹佩雪,等.不同工艺制备的升麻葛根汤中七种成分的含量测定[J].中国现代医学杂志,2013,23(16):6-10.
[18]高鸿彬,傅芃,王晓君,等.HPLC法测定养阴清肺口服液中芍药苷的含量[J].西北药学杂志,2016,31(3):254-256.
[19]李鑫,张秀丽.高效液相色谱法同时测定人参养荣丸中芍药苷、阿魏酸和橙皮苷含量[J].中国药业,2016,25(17):68-70.
[20]国家药典委员会.中国药典:2015年版:一部[S].北京:中国医药科技出版社,2015:105-106.
[21]张士勇.芪丹芍龙合剂中芍药苷含量测定方法的研究[J].中医药临床杂志,2012,24(1):71-72.