高效液相色谱法测定养阴清肺口服液中毛蕊花糖苷的含量
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摘要
目的用高效液相色谱法测定养阴清肺口服液中毛蕊花糖苷的含量。方法色谱柱为Kromasil C18(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%醋酸溶液(16∶84),检测波长为334 nm,流速为1.0 ml/min,柱温为28℃。结果毛蕊花糖苷进样量在10.12~253.00μg(r=0.9995)线性范围内线性关系良好,平均回收率为99.68%,RSD=0.8%(n=6)。结论高效液相色谱法方法准确,重复性好,可用于养阴清肺口服液的质量控制。
Objective To determine the contents of verbascoside in Yangyinqingfei oral solution by high performance liquid chromatography(HPLC).Methods The column was Kromasil C18(4.6 mm×250 mm,5 μm).The mobile phase was a mixture of acetonitrile-0.1% acetic acid solution(16∶84) at a flow rate of 1.0 ml/min,and the detecting wavelength was at 334 nm,the temperature was 28℃.Rusults The linear range of verbascoside was 10.12-253.00 μg(r=0.9995) and the average recovery rate was 99.68%,RSD=0.8%(n=6).Conclusion The method is accurate with a good reproducibility,and suitable for quality control of Yangyinqingfei oral solution.
Objective To determine the contents of verbascoside in Yangyinqingfei oral solution by high performance liquid chromatography(HPLC).Methods The column was Kromasil C18(4.6 mm×250 mm,5 μm).The mobile phase was a mixture of acetonitrile-0.1% acetic acid solution(16∶84) at a flow rate of 1.0 ml/min,and the detecting wavelength was at 334 nm,the temperature was 28℃.Rusults The linear range of verbascoside was 10.12-253.00 μg(r=0.9995) and the average recovery rate was 99.68%,RSD=0.8%(n=6).Conclusion The method is accurate with a good reproducibility,and suitable for quality control of Yangyinqingfei oral solution.
引文
[1]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2010:115.
[2]周福成.高效液相色谱图集[M].沈阳:辽宁科学技术出版社,2011:76.
[3]莫迎.裸花紫珠制剂中毛蕊花糖苷含量测定[J].医药导报,2012,31(2):245-247.
[4]朱振兴,段红福.高效液相色谱法测定地黄中梓醇及毛蕊花糖苷的含量[J].中医学报,2013,28(7):1023-1024.
[5]吴竞妮.高效液相色谱法测定消郁舒心胶囊中毛蕊花糖苷含量[J].内蒙古中医药,2013,32(11):50-51.
[6]邱广仁,赵向阳.HPLC法测定咳嗽枇杷糖浆中京尼平苷酸和毛蕊花糖苷[J].安微医药,2013,17(4):573-574.
[7]樊琪.左归丸中毛蕊花糖苷含量测定[J].中国科技纵横,2012,(6):254.
[8]张贵财,朱旭江,杜兴,等.HPLC法测定兰州肉苁蓉中毛蕊花糖苷的含量[J].中国药事,2010,24(8):801-803.
[9]张岩.高效液相色谱法测定宽肠理气汤中松果菊苷和毛蕊花糖苷[J].中草药,2010,41(12):1994-1995.
[10]邱倬星,熊学敏,胡晓艳,等.车前子超微速溶饮片中京尼平苷酸与毛蕊花糖苷的含量测定[J].中国医药科学,2013,3(5):41-43.
[2]周福成.高效液相色谱图集[M].沈阳:辽宁科学技术出版社,2011:76.
[3]莫迎.裸花紫珠制剂中毛蕊花糖苷含量测定[J].医药导报,2012,31(2):245-247.
[4]朱振兴,段红福.高效液相色谱法测定地黄中梓醇及毛蕊花糖苷的含量[J].中医学报,2013,28(7):1023-1024.
[5]吴竞妮.高效液相色谱法测定消郁舒心胶囊中毛蕊花糖苷含量[J].内蒙古中医药,2013,32(11):50-51.
[6]邱广仁,赵向阳.HPLC法测定咳嗽枇杷糖浆中京尼平苷酸和毛蕊花糖苷[J].安微医药,2013,17(4):573-574.
[7]樊琪.左归丸中毛蕊花糖苷含量测定[J].中国科技纵横,2012,(6):254.
[8]张贵财,朱旭江,杜兴,等.HPLC法测定兰州肉苁蓉中毛蕊花糖苷的含量[J].中国药事,2010,24(8):801-803.
[9]张岩.高效液相色谱法测定宽肠理气汤中松果菊苷和毛蕊花糖苷[J].中草药,2010,41(12):1994-1995.
[10]邱倬星,熊学敏,胡晓艳,等.车前子超微速溶饮片中京尼平苷酸与毛蕊花糖苷的含量测定[J].中国医药科学,2013,3(5):41-43.