基于遗传算法的中医药治疗肺癌核心有效处方的发现及实验研究
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摘要
目的:探讨中医药治疗肺癌核心处方的数据挖掘方法,对核心处方进行有效性评价。方法:收集300例非小细胞肺癌患者,利用遗传算法发现核心中药处方,通过建立C57BL/6近交系小鼠Lewis肺癌动物模型进行核心处方的有效性评价。结果:挖掘出核心有效处方4张(P<0.05),动物实验表明核心处方可抑制Lewis肺癌皮下移植瘤的瘤体生长,瘤重较对照组减轻(P<0.05),同时联合组对小鼠肿瘤负荷抑制最明显,瘤重最轻。结论:遗传算法是中医药治疗肺癌核心处方数据挖掘的有效方法,挖掘出的核心处方经初步验证有明确疗效。
Objective: To investigate the data mining method applicable for exploring the core prescription in treating lung cancer with traditional Chinese medicine(TCM) and test the curative effect of the core prescription. Methods: After collecting 300 cases of patients with non-small cell lung cancer, we discovered the core prescription through the genetic algorithm, and then we tested the curative effect of the core prescription by establishing the Lewis lung cancer animal model of C57 BL/6 inbred mice. Results: We discovered four core effective prescription(P<0.05). Animal experiments shown core prescriptions were proved to inhibit the growth of the subcutaneous transplantation tumor of Lewis lung cancer, tumor weight was lighter than that of the control group(P<0.05), at the same time, the combined group had the most obvious inhibition on tumor load in mice, and the tumor weight was the lightest. Conclusion: Genetic algorithm, a kind of the data mining method, has proved effective to discover the core prescription in treating lung cancer with TCM. Meanwhile, the core prescription we discovered has proved clearly effective by preliminary verification.
Objective: To investigate the data mining method applicable for exploring the core prescription in treating lung cancer with traditional Chinese medicine(TCM) and test the curative effect of the core prescription. Methods: After collecting 300 cases of patients with non-small cell lung cancer, we discovered the core prescription through the genetic algorithm, and then we tested the curative effect of the core prescription by establishing the Lewis lung cancer animal model of C57 BL/6 inbred mice. Results: We discovered four core effective prescription(P<0.05). Animal experiments shown core prescriptions were proved to inhibit the growth of the subcutaneous transplantation tumor of Lewis lung cancer, tumor weight was lighter than that of the control group(P<0.05), at the same time, the combined group had the most obvious inhibition on tumor load in mice, and the tumor weight was the lightest. Conclusion: Genetic algorithm, a kind of the data mining method, has proved effective to discover the core prescription in treating lung cancer with TCM. Meanwhile, the core prescription we discovered has proved clearly effective by preliminary verification.
引文
[1]陈万青,孙可欣,郑荣寿,等.2014年中国分地区恶性肿瘤发病和死亡分析.中国肿瘤,2017,27(1):1-14
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[12]成泽东,陈以国.针刺联合紫杉醇对小鼠Lewis肺癌细胞凋亡的影响.针刺研究,2007,32(3):153-157
[13]Holland J H.Adaptation in Natural and Artificial Systems:An Introductory Analysis with Applications to Biology,Control,and Artificial Intelligence.2nd edition.Cambridge:MIT Press,1992
[14]刘嘉湘,施志明,李和根,等.益肺抗瘤饮治疗271例非小细胞肺癌临床观察.上海中医药杂志,2001,32(3):3-6
[15]Yeon Hee Choi,Dae Seok Yoo,Shi Yong Ryu,et al.Antiproliferative effects of saponins from the roots of platycodon grandiflorum on cultured human tumor cells.J Nat Prod,2010,73(11):1863-1867
[16]Liang Feng,Xiao-Bin Jia,Yan Chen,et al.Identification of two polysaccharides from Prunella vulgaris L.and evaluation on their anti-lung adenocarcinoma activity.Molecules,2010,15(8):5093-5103
[17]马丁丁,陈艳,黄尤光,等.淫羊藿素通过PI3K/AKT信号通路抑制非小细胞肺癌H460细胞增殖.现代肿瘤医学,2016,27(14):2180-2183
[18]Hai-ming Wei,Rui Sun,Zhi-gang Tian,et al.Traditional Chinese medicine Astragalus reverses predominance of Th2 cytokines and their up-stream transcript factors in lung cancer patients.Oncology Research,2003,10(5):1507-1512
[19]柏长青,遆新宇,刘关键.党参、黄芪提取物注射液对减轻肺癌患者化疗毒副反应和生存质量影响的研究.中国康复医学杂志,2006,21(8):707-709
[2]Rebecca L,Siegel M P H,Kimberly D,et al.Cancer statistics,2018.CA Cancer J Clin,2018,68(1):7-30
[3]B K Piao,Y X Wang,H S Lin,et al.Impact of complementary mistletoe extract treatment on quality of life in breast,ovarian and non-small cell lung cancer patients:A prospective randomized controlled clinical trial.Anticancer Research,2004,24(1):303-310
[4]贾英杰,黄敏娜,孙一予,等.厄洛替尼联合消岩汤加减方治疗非小细胞肺癌的临床观察.临床肿瘤学杂志,2009,14(7):622-624
[5]Zhong-liang Liu,Wei-rong Zhu,Xiao-heng Shen,et al.Traditional Chinese medicinal herbs combined with epidermal growth factor receptor tyrosine kinase inhibitor for advanced non-small cell lung cancer:a systematic review and meta-analysis.Journal of Integrative Medicine,2014,12(4):346-358
[6]任建业,许鸣,陆嘉惠.基于数据挖掘的中医临床用药规律和证型研究进展.中华中医药杂志,2017,32(10):4579-4582
[7]杨凤珍,刘宗莲,张小平,等.基于路志正医案数据挖掘现代疾病内伤湿浊病机与治法研究.中华中医药杂志,2017,32(9):4252-4255
[8]中华人民共和国卫生部.中国常见恶性肿瘤治疗规范.北京:北京医科大学、中国协和医科大学联合出版社,1991
[9]李仪奎.中药药理实验方法学.2版.上海:上海科技出版社,2006:1063
[10]D Beasley,D R Bull,R R Martin,et al.An overview of genetic algorithms:Part1 fundamentals.University Computing,1993,15(2):58-69
[11]Ming Yang,Josiah Poon,Ling Xu,et al.Application of genetic algorithm for discovery of core effective formulae in TCM clinical data.Comput Math Method,2013:971272
[12]成泽东,陈以国.针刺联合紫杉醇对小鼠Lewis肺癌细胞凋亡的影响.针刺研究,2007,32(3):153-157
[13]Holland J H.Adaptation in Natural and Artificial Systems:An Introductory Analysis with Applications to Biology,Control,and Artificial Intelligence.2nd edition.Cambridge:MIT Press,1992
[14]刘嘉湘,施志明,李和根,等.益肺抗瘤饮治疗271例非小细胞肺癌临床观察.上海中医药杂志,2001,32(3):3-6
[15]Yeon Hee Choi,Dae Seok Yoo,Shi Yong Ryu,et al.Antiproliferative effects of saponins from the roots of platycodon grandiflorum on cultured human tumor cells.J Nat Prod,2010,73(11):1863-1867
[16]Liang Feng,Xiao-Bin Jia,Yan Chen,et al.Identification of two polysaccharides from Prunella vulgaris L.and evaluation on their anti-lung adenocarcinoma activity.Molecules,2010,15(8):5093-5103
[17]马丁丁,陈艳,黄尤光,等.淫羊藿素通过PI3K/AKT信号通路抑制非小细胞肺癌H460细胞增殖.现代肿瘤医学,2016,27(14):2180-2183
[18]Hai-ming Wei,Rui Sun,Zhi-gang Tian,et al.Traditional Chinese medicine Astragalus reverses predominance of Th2 cytokines and their up-stream transcript factors in lung cancer patients.Oncology Research,2003,10(5):1507-1512
[19]柏长青,遆新宇,刘关键.党参、黄芪提取物注射液对减轻肺癌患者化疗毒副反应和生存质量影响的研究.中国康复医学杂志,2006,21(8):707-709