内切纤维素酶改善ONP浆滤水性研究及应用
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摘要
探讨了内切纤维素酶TM3性能及改善ONP浆料滤水性的应用工艺。结果表明:内切纤维素酶TM3的最适温度为50℃,最适p H为7.0,在p H6.0~8.0范围内有良好的酶活特性。内切纤维素酶TM3改善ONP浆料滤水性的最佳工艺:预处理时间为60 min,浆料浓度3.0%,用量为0.05μ/g(绝干浆)。与常规工艺相比,经内切纤维素酶TM3处理ONP浆打浆度下降2.0°SR,成纸环压指数、耐折度有所提高,底驱网辊电流降低0.91%。
The function of endo-cellulase TM3 and its application on improving drainage of ONP pulp were studied. The results show that: the optimum temperature of endo-cellulase TM3 was 50 ℃,the optimum p H was 7. 0. When the p H value was between 6. 0~8. 0,the enzyme had a good activity. The optimum conditions for improving drainage of ONP pulp were obtained as follows: pulp pretreatment time 60 min,pulp pretreatment consistency 3. 0%,enzyme dosage 0. 05 μ/g( o. d. pulp). Compared with the conventional process,the beating degree of the pulp pretreated by endo-cellulase TM3 decreased 2. 0 oSR,ring crush index and folding endurance were improved,drive roller current of bottom web decreased 0. 91%.
The function of endo-cellulase TM3 and its application on improving drainage of ONP pulp were studied. The results show that: the optimum temperature of endo-cellulase TM3 was 50 ℃,the optimum p H was 7. 0. When the p H value was between 6. 0~8. 0,the enzyme had a good activity. The optimum conditions for improving drainage of ONP pulp were obtained as follows: pulp pretreatment time 60 min,pulp pretreatment consistency 3. 0%,enzyme dosage 0. 05 μ/g( o. d. pulp). Compared with the conventional process,the beating degree of the pulp pretreated by endo-cellulase TM3 decreased 2. 0 oSR,ring crush index and folding endurance were improved,drive roller current of bottom web decreased 0. 91%.
引文
[1]SARKAR J M,COSPER D R,HARTIG EJ.Applying enzymes and polymers to enhance the freeness of recycled fiber[J].Tappi Journal,1995,78(2):89-95.
[2]JACKSON L S,HEITMANN J A,JOYCE T W.Enzymatic modifications of secondary fiber[J].Tappi Journal,1993,76(3):147-154.
[3]董毅.酶在纸浆抄造过程中的助滤助留和增强作用[D].济南:山东轻工业学院,2009.
[4]DIENESD,EGYHAZI A,RDCZEY K.Treatment of recycled fiber with trichoderma cellulases[J].Industrial Crops and Products,2004,20:11-21.
[5]CARRARD Q,KOIVULA A SODEFLUND H AND BEGUIN E.Cellulose-binding domains promote hydrolysis of different sites on crystalline cellulose.Proceedings of National Academy of Sciences USA.2000,97(19):10342-10347.
[6]阎伯旭,孙迎庆,高培基.有限酶切拟康氏木霉纤维素酶分子研究其结构域的结构与功能[J].纤维素科学与技术,1998,6(3):2-9.
[7]NATIVEDAD ORTEGA,MARIA D,BUSTO.Kinetics of cellulose saccharification by Trichoderma reesei cellulases[J].Inernational Biodeterioration and Biodegradation,2001,47:7-14.
[8]张素风,王双飞,安郁琴.酶对二次纤维性能的改善[J].中国造纸学报,2004,19(2):84-87.
[9]吴芹,陈嘉川,王双飞.酶法改善杨木APMP浆滤水性能的研究[J].中华纸业,2010,31(8):26-27.
[10]OLTUSE,MATO J,BAUER S,et al.Enzymatic hydrolysis of waste paper[J].Cellulose Chemistry and Technology,1987,(21):663.
[11]MORAN BRIAN R.Enzyme treatment improves refining efficiency,recycled fiber freeness[J].Pulp and Paper,1996,(9):119.
[12]张爱萍,秦梦华,徐清华.酶对纤维改性的研究进展[J].中国造纸,2005,24(9):57-60.
[2]JACKSON L S,HEITMANN J A,JOYCE T W.Enzymatic modifications of secondary fiber[J].Tappi Journal,1993,76(3):147-154.
[3]董毅.酶在纸浆抄造过程中的助滤助留和增强作用[D].济南:山东轻工业学院,2009.
[4]DIENESD,EGYHAZI A,RDCZEY K.Treatment of recycled fiber with trichoderma cellulases[J].Industrial Crops and Products,2004,20:11-21.
[5]CARRARD Q,KOIVULA A SODEFLUND H AND BEGUIN E.Cellulose-binding domains promote hydrolysis of different sites on crystalline cellulose.Proceedings of National Academy of Sciences USA.2000,97(19):10342-10347.
[6]阎伯旭,孙迎庆,高培基.有限酶切拟康氏木霉纤维素酶分子研究其结构域的结构与功能[J].纤维素科学与技术,1998,6(3):2-9.
[7]NATIVEDAD ORTEGA,MARIA D,BUSTO.Kinetics of cellulose saccharification by Trichoderma reesei cellulases[J].Inernational Biodeterioration and Biodegradation,2001,47:7-14.
[8]张素风,王双飞,安郁琴.酶对二次纤维性能的改善[J].中国造纸学报,2004,19(2):84-87.
[9]吴芹,陈嘉川,王双飞.酶法改善杨木APMP浆滤水性能的研究[J].中华纸业,2010,31(8):26-27.
[10]OLTUSE,MATO J,BAUER S,et al.Enzymatic hydrolysis of waste paper[J].Cellulose Chemistry and Technology,1987,(21):663.
[11]MORAN BRIAN R.Enzyme treatment improves refining efficiency,recycled fiber freeness[J].Pulp and Paper,1996,(9):119.
[12]张爱萍,秦梦华,徐清华.酶对纤维改性的研究进展[J].中国造纸,2005,24(9):57-60.