七叶皂苷凝胶对宫颈炎大鼠的治疗作用
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摘要
目的探讨七叶皂苷凝胶剂对于宫颈炎模型大鼠的治疗作用。方法利用醇提方法从梭罗果中分离纯化获得七叶皂苷并制备成凝胶剂;利用25%苯酚胶浆在SD大鼠中诱导宫颈炎模型,随后局部注入七叶皂苷凝胶进行治疗,观察模型大鼠阴道口红肿情况及分泌物量的变化,取宫颈组织石蜡包埋后切片,HE染色观察组织病理改变。结果七叶皂苷凝胶剂可明显加快大鼠宫颈炎模型动物损伤修复,显著减轻大鼠阴道口红肿及分泌物。HE染色表明七叶皂苷凝胶剂处理后大鼠的损伤部位炎性细胞浸润明显减少。结论七叶皂苷钠凝胶剂对于大鼠宫颈炎有着良好的疗效,有望用于宫颈炎的治疗。
Objective To investigate the therapeutic effect of aescin gel on cervicitis in rats. Methods Sodium aescinate was extracted from Chinese buckeye seeds by alcohol extraction method and prepared into a gel. The cervicitis model was induced in SD rats by 25% phenolic glue injection and aescinate gel was local administrated to treat the rats. After treatment, the changes of vaginal redness and secretion of the model rats were observed. The paraffin-embedded sections of the cervical tissues were taken and HE staining was used to observe histopathological changes. Results Aescin gel significantly accelerated the damage repair of model animals. It significantly reduced the vaginal swelling and secretion in rats. HE staining showed that the infiltration of inflammatory cells in the injured sites in rats was significantly reduced after treatment with aescin. Conclusion Sodium aescinate gel has a good effect on cervicitis rats. It is expected to be used for the treatment of cervicitis.
Objective To investigate the therapeutic effect of aescin gel on cervicitis in rats. Methods Sodium aescinate was extracted from Chinese buckeye seeds by alcohol extraction method and prepared into a gel. The cervicitis model was induced in SD rats by 25% phenolic glue injection and aescinate gel was local administrated to treat the rats. After treatment, the changes of vaginal redness and secretion of the model rats were observed. The paraffin-embedded sections of the cervical tissues were taken and HE staining was used to observe histopathological changes. Results Aescin gel significantly accelerated the damage repair of model animals. It significantly reduced the vaginal swelling and secretion in rats. HE staining showed that the infiltration of inflammatory cells in the injured sites in rats was significantly reduced after treatment with aescin. Conclusion Sodium aescinate gel has a good effect on cervicitis rats. It is expected to be used for the treatment of cervicitis.
引文
[1]Zhou XY,Fu FH,Li Z,et al.Escin,a natural mixture of triterpene saponins,exhibits antitumor activity against hepatocellular carcinoma[J].Planta Med,2009,75(15):1580-5.
[2]Chen J,Li W,Yang B,et al.Determination of four major saponins in the seeds of Aesculus chinensis Bunge using accelerated solvent extraction followed by high-performance liquid chromatography and electrospray-time of flight mass spectrometry[J].Anal Chim Acta,2007,596(2):273-80.
[3]Abudayeh ZH,Al AK,Naddaf A,et al.Determination of four major saponins in skin and endosperm of seeds of horse chestnut(aesculus hippocastanum L.)using high performance liquid chromatography with positive confirmation by thin layer chromatography[J].Adv Pharm Bull,2015,5(4):587-91.
[4]任捷,吴彦,刘海平.七叶皂苷钠片治疗外伤性肢体肿胀的临床观察[J].中国基层医药,2011,18(2):38-9.
[5]Wang T,Jiang N,Han B,et al.Escin attenuates cerebral edemainduced by acute omethoate poisoning[J].Toxicol Mech Methods,2011,21(5):400-5.
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[7]Paavonen J,Kiviat N,Brunham RC,et al.Prevalence and manifestations of endometritis among women with cervicitis[J].Am J Obstet Gynecol,1985,152(3):280-6.
[8]Rosenfeld WD,Clark J.Vulvovaginitis and cervicitis[J].Pediatr Clin North Am,1989,36(3):489-511.
[9]Critchlow CW,Wolner-Hanssen P,Eschenbach D,et al.Determinants of cervical ectopia and of cervicitis:age,oral contraception,specific cervicalinfection,smoking,and douching[J].Am J Obstet Gynecol,1995,173(2):534-43.
[10]Hashmi S,Begum W,Sultana A.Efficacy of sphaeranthus indicus and cream of lawsonia inermis in cervical erosion with cervicitis[J].EurJ IntegrMed,2011,3(3):e183-8.
[11]George M,Joseph L,Sujith K,et al.Sphaeranthus indicus Linn:a pharmacological update[J].J Pharm Innov,2017,6(2):77-84.
[12]Srivastava S,Shahi UP,Dibya A,et al.Distribution of HPV genotypes and involvement of risk factors in cervical lesions and invasive cervical cancer:a study in an Indian population[J].Int J Mol Cell Med,2014,3(2):61-73.
[13]Mcclelland RS,Wang CC,Mandaliya K,et al.Treatment of cervicitis is associated with decreased cervical shedding of HIV-1[J].AIDS,2001,15(1):105-10.
[14]Li M,Lu C,Zhang L,et al.Oral administration of escin inhibits acute inflammation and reduces intestinal mucosal injury in animal models[J].Evid-Based Compl,2015,21(5):503617-29.
[15]Maximiliano SF,Vazquez L,Virginia GM,et al.Aesculus hippocastanuml seed extract shows virucidal and antiviral activities against respiratory syncytial virus(RSV)and reduces lung for updates inflammation in vivo[J].Antiviral Res,2019,164(164):1-11.
[16]Zhao SQ,Xu SQ,Cheng J,et al.Anti-inflammatory effect of external use of escin on cutaneous inflammation:possible involvement of glucocorticoids receptor[J].Chin J Nat Med,2018,16(2):105-12.
[17]Wang B,Mao X,Zhu J.β-aescin alleviates acute lung injury induced by lipopolysaccharide by inhibiting lipid peroxidation and inflammation in mice[J].Chin J Cell Mol Imm,2018,34(7):600-4.
[18]Selvakumar GP,Janakiraman UA,Thenmozhi AJ,et al.Escin attenuates behavioral impairments,oxidative stress and inflammation in a chronic MPTP/probenecid mouse model of Parkinson's disease[J].Brain Res,2014,1585(1):23-36.
[19]Wu XJ,Cui XY,Tian LT,et al.Pharmacokinetics of escin la in rats after intravenous administration[J].J Ethnopharmacol,2014,156(2):125-9.
[20]Magliulo E,Carco FP,Gorini S,et al.Research in vivo and in vitro of the antiphlogistic action of escine[J].Arch Sci Med(Torino),1968,125(6):207-18.
[21]Maghsoudi H,Hallajzadeh J,Rezaeipour M.Evaluation of the effect of polyphenol of escin compared with ibuprofen and dexamethasone in synoviocyte model for osteoarthritis:an in vitro study[J].Clin Rheumatol,2018,37(9):2471-8.
[22]Du Y,Song YQ,Zhang LM,et al.Combined treatment with low dose prednisone and escin improves the anti-arthritic effect in experimental arthritis[J].Int Immunopharmacol,2016,3 1(3):257-65.
[23]Zhang HY,Yang B.Successful treatment of pachydermoperiostosis patients with etoricoxib,aescin,and arthroscopic synovectomy two case reports[J].Medicine(Baltimore),2017,96(47):e8865-77.
[24]Giordano PL,Invernizzi G,Vitali A.Studies on the prevention of cerebral edema due to experimental inflammation:action of escine,sodium salt[J].Riv Sper Freniatr Med Leg Alien Ment,1968,92(5):1543-50.
[2]Chen J,Li W,Yang B,et al.Determination of four major saponins in the seeds of Aesculus chinensis Bunge using accelerated solvent extraction followed by high-performance liquid chromatography and electrospray-time of flight mass spectrometry[J].Anal Chim Acta,2007,596(2):273-80.
[3]Abudayeh ZH,Al AK,Naddaf A,et al.Determination of four major saponins in skin and endosperm of seeds of horse chestnut(aesculus hippocastanum L.)using high performance liquid chromatography with positive confirmation by thin layer chromatography[J].Adv Pharm Bull,2015,5(4):587-91.
[4]任捷,吴彦,刘海平.七叶皂苷钠片治疗外伤性肢体肿胀的临床观察[J].中国基层医药,2011,18(2):38-9.
[5]Wang T,Jiang N,Han B,et al.Escin attenuates cerebral edemainduced by acute omethoate poisoning[J].Toxicol Mech Methods,2011,21(5):400-5.
[6]Lusk MJ,Konecny P.Cervicitis:a review[J].Curr Opin Infect Dis,2008,21(1):49-55.
[7]Paavonen J,Kiviat N,Brunham RC,et al.Prevalence and manifestations of endometritis among women with cervicitis[J].Am J Obstet Gynecol,1985,152(3):280-6.
[8]Rosenfeld WD,Clark J.Vulvovaginitis and cervicitis[J].Pediatr Clin North Am,1989,36(3):489-511.
[9]Critchlow CW,Wolner-Hanssen P,Eschenbach D,et al.Determinants of cervical ectopia and of cervicitis:age,oral contraception,specific cervicalinfection,smoking,and douching[J].Am J Obstet Gynecol,1995,173(2):534-43.
[10]Hashmi S,Begum W,Sultana A.Efficacy of sphaeranthus indicus and cream of lawsonia inermis in cervical erosion with cervicitis[J].EurJ IntegrMed,2011,3(3):e183-8.
[11]George M,Joseph L,Sujith K,et al.Sphaeranthus indicus Linn:a pharmacological update[J].J Pharm Innov,2017,6(2):77-84.
[12]Srivastava S,Shahi UP,Dibya A,et al.Distribution of HPV genotypes and involvement of risk factors in cervical lesions and invasive cervical cancer:a study in an Indian population[J].Int J Mol Cell Med,2014,3(2):61-73.
[13]Mcclelland RS,Wang CC,Mandaliya K,et al.Treatment of cervicitis is associated with decreased cervical shedding of HIV-1[J].AIDS,2001,15(1):105-10.
[14]Li M,Lu C,Zhang L,et al.Oral administration of escin inhibits acute inflammation and reduces intestinal mucosal injury in animal models[J].Evid-Based Compl,2015,21(5):503617-29.
[15]Maximiliano SF,Vazquez L,Virginia GM,et al.Aesculus hippocastanuml seed extract shows virucidal and antiviral activities against respiratory syncytial virus(RSV)and reduces lung for updates inflammation in vivo[J].Antiviral Res,2019,164(164):1-11.
[16]Zhao SQ,Xu SQ,Cheng J,et al.Anti-inflammatory effect of external use of escin on cutaneous inflammation:possible involvement of glucocorticoids receptor[J].Chin J Nat Med,2018,16(2):105-12.
[17]Wang B,Mao X,Zhu J.β-aescin alleviates acute lung injury induced by lipopolysaccharide by inhibiting lipid peroxidation and inflammation in mice[J].Chin J Cell Mol Imm,2018,34(7):600-4.
[18]Selvakumar GP,Janakiraman UA,Thenmozhi AJ,et al.Escin attenuates behavioral impairments,oxidative stress and inflammation in a chronic MPTP/probenecid mouse model of Parkinson's disease[J].Brain Res,2014,1585(1):23-36.
[19]Wu XJ,Cui XY,Tian LT,et al.Pharmacokinetics of escin la in rats after intravenous administration[J].J Ethnopharmacol,2014,156(2):125-9.
[20]Magliulo E,Carco FP,Gorini S,et al.Research in vivo and in vitro of the antiphlogistic action of escine[J].Arch Sci Med(Torino),1968,125(6):207-18.
[21]Maghsoudi H,Hallajzadeh J,Rezaeipour M.Evaluation of the effect of polyphenol of escin compared with ibuprofen and dexamethasone in synoviocyte model for osteoarthritis:an in vitro study[J].Clin Rheumatol,2018,37(9):2471-8.
[22]Du Y,Song YQ,Zhang LM,et al.Combined treatment with low dose prednisone and escin improves the anti-arthritic effect in experimental arthritis[J].Int Immunopharmacol,2016,3 1(3):257-65.
[23]Zhang HY,Yang B.Successful treatment of pachydermoperiostosis patients with etoricoxib,aescin,and arthroscopic synovectomy two case reports[J].Medicine(Baltimore),2017,96(47):e8865-77.
[24]Giordano PL,Invernizzi G,Vitali A.Studies on the prevention of cerebral edema due to experimental inflammation:action of escine,sodium salt[J].Riv Sper Freniatr Med Leg Alien Ment,1968,92(5):1543-50.