感染H3N2亚型SwIV荣昌仔猪组织脏器的损伤及其载毒分析
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摘要
【目的】明确H3N2型SwIV对仔猪组织脏器引起的损伤变化及其在组织中的载毒情况。【方法】试验采用H3N2型SwIV接种鸡胚扩增病毒,Reed-Muench法检测病毒EID_(50),滴鼻感染H1N1和H3N2抗体为阴性的仔猪,5 d后剖解观察仔猪临床病理变化,RT-PCR检测病毒在主要组织脏器中的载毒情况。【结果】感染H3N2型SwIV仔猪在5 d内未出现典型的临床症状,但是其肺脏组织有明显淤血和损伤坏死,肺泡壁增厚,肺泡内有纤维性渗出物和炎症细胞的聚集。组织中载毒检测结果显示主要载毒于肺脏组织中。【结论】感染H3N2型SwIV仔猪不一定出现典型临床症状,但其组织受到严重损伤,且主要载毒于肺脏组织。
【Objective】 The present paper aimed to identify the tissue viscera damage and its toxicity in Rongchang piglet infected H3 N2 swine influenza virus. 【Method】The chicken embryos were inoculated H3 N2 swine influenza virus for amplifying virus, detecting EID_(50) by reed-muench method and H1 N1 and H3 N2 antibody negative piglets inoculated H3 N2 swine influenza virus by drip nose. After 5 days, the clinicopathological changes of piglets by caesarean section were observed and the virus' s toxicity in the main tissue organs was detected with RT-PCR.【Result】The piglets did not show typical clinical infection symptoms within 5 days with H3 N2 swine influenza virus, but their lungs appeared obvious congestion and necrosis, alveolar wall increased, and the fibrotic exudation and inflammatory cells were aggregated. The result indicated that the main virus carryed tissue was lung. 【Conclusion】The piglets infected H3 N2 swine influenza virus did not necessarily appear typical clinical symptoms, but their tissues were severely damaged, the lung was virus carried tissue.
【Objective】 The present paper aimed to identify the tissue viscera damage and its toxicity in Rongchang piglet infected H3 N2 swine influenza virus. 【Method】The chicken embryos were inoculated H3 N2 swine influenza virus for amplifying virus, detecting EID_(50) by reed-muench method and H1 N1 and H3 N2 antibody negative piglets inoculated H3 N2 swine influenza virus by drip nose. After 5 days, the clinicopathological changes of piglets by caesarean section were observed and the virus' s toxicity in the main tissue organs was detected with RT-PCR.【Result】The piglets did not show typical clinical infection symptoms within 5 days with H3 N2 swine influenza virus, but their lungs appeared obvious congestion and necrosis, alveolar wall increased, and the fibrotic exudation and inflammatory cells were aggregated. The result indicated that the main virus carryed tissue was lung. 【Conclusion】The piglets infected H3 N2 swine influenza virus did not necessarily appear typical clinical symptoms, but their tissues were severely damaged, the lung was virus carried tissue.
引文
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[12]李钰,刘佳,韦丹丹,等. 动物病理组织切片制作方法改良的探讨[J]. 黑龙江畜牧兽医,2014(15):154-157.
[13]石慧花. 国内部分地区样品猪流感H1、H3感染状况调查分析[D]. 上海:上海交通大学硕士学位论文,2015.
[14]PACCHA B,JONES R M,GIBBS S,et al. Modeling risk of occupational zoonotic influenza infection in swine workers[J]. J Occup Environ Hyg,2016,13(8):577-587.
[2]DAI X Q,LIU M,ZHANG T H,et al. Clinical Predictors for Diagnosing Pandemic (H1N1) 2009 and Seasonal Influenza(H3N2)in Fever Clinics in Beijing,China[J]. Biomed Environ Sci,2012,25(1):61-68.
[3]JO S K,KIM H S,CHO S W,et al. Pathogenesis and inflammatory responses of swine H1N2 influenza viruses in pigs[J]. Virus Res,2007,129(1):64-70.
[4]KIM B,AHN K K,HAN Y,et al. Association of tumor necrosis factor-alpha with fever and pulmonary lesion score in pigs experementally infected with swine influenza virus subtype H1N2[J]. J Vet Med Sci,2009,71(5):611-616.
[5]BARBE F, ATANASOVA K,VAN R K. Cytokines and acute phase proteins association with acute swine influenza infection in pigs[J]. Vet J,2011,187(1):48-53.
[6]白昀,舒采松,甘源,等. 猪流感H3N2型病毒特异性sIgA抗体分泌规律研究[J].中国人畜共患病学报,2017,33(11):956-962.
[7]祁贤,姚火春,陆承平. 猪流行性感冒病毒H3N2亚型体外诱导猪肺巨噬细胞发生凋亡[J]. 微生物与感染,2009(2):76-81.
[8]方斌,余晓,李翔,等. 2010-2015年湖北省H3N2亚型流感病毒抗原漂移分析[J].疾病监测,2017,32(5):387-393.
[9]孙海波,刘双,王璐璐,等. 辽宁省2014-2016年季节性A(H3N2)流感病毒耐药基因分析[J]. 中国卫生检验杂志,2018,28(2):157-159.
[10]杨柳,翟少钦,杨睿,等. H3N2亚型猪流感病毒重庆株的分离、鉴定、基因组测定及序列分析[J]. 中国兽医学报,2015,35(3):359-367.
[11]殷震,刘景华. 动物病毒学(第2版)[M]. 北京:科学出版社,1997.
[12]李钰,刘佳,韦丹丹,等. 动物病理组织切片制作方法改良的探讨[J]. 黑龙江畜牧兽医,2014(15):154-157.
[13]石慧花. 国内部分地区样品猪流感H1、H3感染状况调查分析[D]. 上海:上海交通大学硕士学位论文,2015.
[14]PACCHA B,JONES R M,GIBBS S,et al. Modeling risk of occupational zoonotic influenza infection in swine workers[J]. J Occup Environ Hyg,2016,13(8):577-587.