莱茵衣藻Chlammydomonas reinhardtii净化酒糟废水研究
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摘要
通过结合废水处理与能源微藻培养,既可以实现废水的无害化处理,也可以为微藻的培养提供营养组分和大量水源.以莱茵衣藻(Chlammydomonas reinhardtii)为实验材料,以酒糟废水为培养基,构建室内微型生态系统,考察了不同处理方法下的酒糟废水接种莱茵衣藻后的总氮(TN)、总磷(TP)、化学需氧量(COD)的去除情况以及微藻蛋白提取情况.结果表明:酒糟废水稀释液初始TN质量浓度位于9.39~30.86 mg·L-1时,废水总氮去除率在70%~80%之间;初始TP质量浓度位于3.45~10.93 mg·L-1时,总磷去除率较高,最高达到86.59%;不同稀释倍数的酒糟废水COD去除率均高于50%.而稀释80倍、50倍以及50倍(氮磷质量浓度比为108∶7)的藻可溶性蛋白增长均比较明显,最大值可达到11.36 mg·L-1,同时也说明通过外加氮、磷调节氮磷的初始质量浓度比至108∶7,可大大提高其产量.总体上,在稀释倍数为50倍(TN、TP初始质量浓度分别为18.23、6.99 mg·L-1)时,莱茵衣藻生长良好,废水中TN、TP、COD去除效果均比较明显,对水质的净化效果最佳,同时可获得较高质量浓度的藻可溶性蛋白,这为酒糟废水与微藻耦合规模化培养提供了一种新的思路.
It could realize innocent treatment of wastewater and provide culture of microalgae with nutrition and water resource by combining wastewater treatment with microalgae culture. In this research,indoor micro ecological system was set by using Chlammydomonas reinhardtii as experimental material and vinasse wastewater as culture medium to observe the removal rate of total nitrogen( TN),total phosphorus( TP) and chemical oxygen demand( COD) in vinasse wastewater,and the effect of microalgae protein extraction as well. The result showed that the final removal rate of TN reached up to 70% to 80% when the initial concentration of TN in vinasse wastewater was between 9. 39 mg·L-1and 30. 86 mg·L-1,it had a good removal rate even to 86. 59% of TP when the initial content of TP was between 3. 45 mg·L-1and 10. 93 mg·L-1. C. reinhardtii could remove more than 50% COD of vinasse wastewater which had different dilution ratio. Meantime,the content of soluble protein increased obviously under the conditions of diluted 80 ×,50 ×,and 50 ×( the rate of nitrogen and phosphorus being 108∶ 7). In general,at the condition of diluted 50 ×( TN,TP concentration being 18. 23 mg·L-1,6. 99 mg·L-1,respectively),C. reinhardtii grows well,and TN,TP,COD removal efficiency are more obvious,the water quality purification effect is the best,and it also can obtain a higher content of soluble protein,which provides a new way of thinking for co-cultivation between vinasse wastewater and microalgae.
It could realize innocent treatment of wastewater and provide culture of microalgae with nutrition and water resource by combining wastewater treatment with microalgae culture. In this research,indoor micro ecological system was set by using Chlammydomonas reinhardtii as experimental material and vinasse wastewater as culture medium to observe the removal rate of total nitrogen( TN),total phosphorus( TP) and chemical oxygen demand( COD) in vinasse wastewater,and the effect of microalgae protein extraction as well. The result showed that the final removal rate of TN reached up to 70% to 80% when the initial concentration of TN in vinasse wastewater was between 9. 39 mg·L-1and 30. 86 mg·L-1,it had a good removal rate even to 86. 59% of TP when the initial content of TP was between 3. 45 mg·L-1and 10. 93 mg·L-1. C. reinhardtii could remove more than 50% COD of vinasse wastewater which had different dilution ratio. Meantime,the content of soluble protein increased obviously under the conditions of diluted 80 ×,50 ×,and 50 ×( the rate of nitrogen and phosphorus being 108∶ 7). In general,at the condition of diluted 50 ×( TN,TP concentration being 18. 23 mg·L-1,6. 99 mg·L-1,respectively),C. reinhardtii grows well,and TN,TP,COD removal efficiency are more obvious,the water quality purification effect is the best,and it also can obtain a higher content of soluble protein,which provides a new way of thinking for co-cultivation between vinasse wastewater and microalgae.
引文
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[11]梁锏文,李智斌,陈柏铨.利用微藻处理生物丁醇废水的初步研究[J].广州化工,2015,22:78-80.
[12]YU S,HUBBARD J S,HOLZER G.Total lipid production of the green of the green alga nannochloropsis sp.Qii under different nitrogen regimes[J].J Phycology,1987,23(23):892-96.
[13]DORTCH Q.Effect of growth conditions on accumulation of internal nitrate,ammonium and protein in three marine diatoms[J].J Experimental Marine Biology Ecology,1982,61(3):43-254.
[14]曾玲,文菁,徐春曼.氮、磷对微藻生长和产毒的影响[J].湛江师范学院学报,2011,32(6):103-108.
[2]李健秀,王建刚,邱俊,等.超滤-反渗透集成工艺处理玉米酒糟废水[J].化学工程,2007,35(8):42-44.
[3]郭庆东,黄杰龙,冠巧花,等.UASB工艺在规模化处理米酒糟废水中的应用[J].水处理技术,2013,39(6):125-128.
[4]李克勋,王太平,张振家.酒糟废水处理工艺改造实例[J].中国给水排水,2004,20(5):83-85.
[5]耿土锁,袁嗣兵.高浓度酒糟废水的综合利用[J].江苏环境科技,1997,2:29-31.
[6]刘磊,杨雪薇,陈朋宇,等.3种微藻对人工污水中氮磷去除效果的研究[J].广东农业科学,2014,11:172-176.
[7]GOUVEIA L,OLIVEIRA A C.Microalgae as a raw material for biofuels production[J].J Ind Microbiol Biot,2008,36(2):269-274.
[8]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[9]邓旭,魏斌,胡章立.利用莱茵衣藻去除污水中氮磷的研究[J].环境科学,2010,31(6):89-93.
[10]章斐,陈秀荣,江子建.不同氮磷浓度下2种无毒微藻的生长特性和脱氮除磷效能[J].环境工程学报,2015,9(2):59-66.
[11]梁锏文,李智斌,陈柏铨.利用微藻处理生物丁醇废水的初步研究[J].广州化工,2015,22:78-80.
[12]YU S,HUBBARD J S,HOLZER G.Total lipid production of the green of the green alga nannochloropsis sp.Qii under different nitrogen regimes[J].J Phycology,1987,23(23):892-96.
[13]DORTCH Q.Effect of growth conditions on accumulation of internal nitrate,ammonium and protein in three marine diatoms[J].J Experimental Marine Biology Ecology,1982,61(3):43-254.
[14]曾玲,文菁,徐春曼.氮、磷对微藻生长和产毒的影响[J].湛江师范学院学报,2011,32(6):103-108.