超声辅助水酶法提取冬瓜籽油的工艺研究
|
摘要
以冬瓜籽为原料,采用超声辅助水酶法提取冬瓜籽油。在单因素实验的基础上进行正交实验,得出最佳提取工艺条件:颗粒粒度60目,液料比7∶1,酶用量2.0%(以冬瓜籽质量计),pH 8.0,酶解温度55℃,超声时间20 min,超声功率150 W,超声温度50℃。在最佳条件下,冬瓜籽油提取率为82.55%。
The Benincasa hispida Cogn. seed oil was extracted by ultrasound-assisted aqueous enzymatic method. On the basis of single factor experiment,the optimal extraction conditions were obtained by orthogonal experiment as follows: partical size 60 meshes,liquid-solid ratio 7 ∶ 1,dosage of alcalase2. 0%( based on the mass of Benincasa hispida Cogn. seed),pH 8. 0,enzymolysis temperature 55 ℃,ultrasonic time 20 min,ultrasonic power 150 W,ultrasonic temperature 50 ℃. Under these conditions,the extraction rate was 82. 55%.
The Benincasa hispida Cogn. seed oil was extracted by ultrasound-assisted aqueous enzymatic method. On the basis of single factor experiment,the optimal extraction conditions were obtained by orthogonal experiment as follows: partical size 60 meshes,liquid-solid ratio 7 ∶ 1,dosage of alcalase2. 0%( based on the mass of Benincasa hispida Cogn. seed),pH 8. 0,enzymolysis temperature 55 ℃,ultrasonic time 20 min,ultrasonic power 150 W,ultrasonic temperature 50 ℃. Under these conditions,the extraction rate was 82. 55%.
引文
[1]吴少福,黎冬明,郑国栋,等.超声辅助提取冬瓜籽油工艺的研究[J].中国粮油学报,2011,26(5):57-60.
[2]吴欣秀,王海凤,李娜,等.冬瓜皮提取物对油脂的抗氧化作用研究[J].粮油食品科技,2016,24(1):37-39.
[3]DEBSANKAR D,SUBHAS M,DEBABRATA M.Structural characterization of dietary fiber of green chalcumra(Benincasa hispida)fruit by NMR spectroscopic analysis[J].Nat Prod Commun,2009,4(4):547-552.
[4]GILL N S,DHIMAN J,BAJWA P.Evaluation of free radical scavenging,anti-inflammatory and analgesic potential of Benincasa hispida seed extract[J].Int J Pharmacol,2010,6(5):652-657.
[5]MOON M K,KANG D G,LEE Y J.Effect of Benincasa hispida Cogniaux on high glucose-induced vascular inflammation of human umbilical vein endothelial cells[J].Vasc Pharmacol,2008,50(3):116-122.
[6]JIANG X,KUANG F,KONG F S,et al.Prediction of the antiglycation activity of polysaccharides from Benincasa hispida using a response surface methodology[J].Carbohydr Polym,2016,151(10):358-363.
[7]程园园,刘大川,刘晔,等.4种油料压榨油的品质评价[J].中国油脂,2016,41(1):7-10.
[8]肖丽娟,曾凡坤.番茄籽油的性质及制取工艺[J].粮油加工,2006(1):45-48.
[9]任飞,韩发,石丽娜,等.超临界CO2萃取技术在植物油脂提取中的应用[J].中国油脂,2010,35(5):14-18.
[10]沈心好,许时婴.超临界CO2萃取番茄籽油[J].无锡轻工大学学报,2004,23(6):1-5.
[11]王强,王存,王睿,等.微波辅助水酶法提取番茄籽油工艺优化及理化特性研究[J].中国粮油学报,2015,30(10):43-48.
[12]严小平,李成平,金建昌.超声波法提取西瓜籽油的最佳工艺研究[J].中国粮油学报,2012,27(3):53-56.
[13]胡滨,陈一资,苏赵.超声波和微波辅助水酶法提取葡萄籽油的工艺研究[J].中国油脂,2015,40(12):12-17.
[14]薛刚,苏印泉,杨芳霞,等.溶剂法提取水冬瓜籽油的研究[J].西北林学院学报,2010,25(3):154-157.
[15]李良玉,曹荣安,于伟,等.超声波辅助提取麦胚黄酮的技术研究[J].粮油食品科技,2014,22(4):42-47.
[16]李伟,陆占国,孙胜敏,等.酸浆果籽油的提取研究[J].食品与机械,2011,27(2):44-46.
[17]方芳,许凯扬,朱强,等.超声波辅助水酶法萃取葫芦籽油的研究[J].中国粮油学报,2012,27(10):53-56.
[2]吴欣秀,王海凤,李娜,等.冬瓜皮提取物对油脂的抗氧化作用研究[J].粮油食品科技,2016,24(1):37-39.
[3]DEBSANKAR D,SUBHAS M,DEBABRATA M.Structural characterization of dietary fiber of green chalcumra(Benincasa hispida)fruit by NMR spectroscopic analysis[J].Nat Prod Commun,2009,4(4):547-552.
[4]GILL N S,DHIMAN J,BAJWA P.Evaluation of free radical scavenging,anti-inflammatory and analgesic potential of Benincasa hispida seed extract[J].Int J Pharmacol,2010,6(5):652-657.
[5]MOON M K,KANG D G,LEE Y J.Effect of Benincasa hispida Cogniaux on high glucose-induced vascular inflammation of human umbilical vein endothelial cells[J].Vasc Pharmacol,2008,50(3):116-122.
[6]JIANG X,KUANG F,KONG F S,et al.Prediction of the antiglycation activity of polysaccharides from Benincasa hispida using a response surface methodology[J].Carbohydr Polym,2016,151(10):358-363.
[7]程园园,刘大川,刘晔,等.4种油料压榨油的品质评价[J].中国油脂,2016,41(1):7-10.
[8]肖丽娟,曾凡坤.番茄籽油的性质及制取工艺[J].粮油加工,2006(1):45-48.
[9]任飞,韩发,石丽娜,等.超临界CO2萃取技术在植物油脂提取中的应用[J].中国油脂,2010,35(5):14-18.
[10]沈心好,许时婴.超临界CO2萃取番茄籽油[J].无锡轻工大学学报,2004,23(6):1-5.
[11]王强,王存,王睿,等.微波辅助水酶法提取番茄籽油工艺优化及理化特性研究[J].中国粮油学报,2015,30(10):43-48.
[12]严小平,李成平,金建昌.超声波法提取西瓜籽油的最佳工艺研究[J].中国粮油学报,2012,27(3):53-56.
[13]胡滨,陈一资,苏赵.超声波和微波辅助水酶法提取葡萄籽油的工艺研究[J].中国油脂,2015,40(12):12-17.
[14]薛刚,苏印泉,杨芳霞,等.溶剂法提取水冬瓜籽油的研究[J].西北林学院学报,2010,25(3):154-157.
[15]李良玉,曹荣安,于伟,等.超声波辅助提取麦胚黄酮的技术研究[J].粮油食品科技,2014,22(4):42-47.
[16]李伟,陆占国,孙胜敏,等.酸浆果籽油的提取研究[J].食品与机械,2011,27(2):44-46.
[17]方芳,许凯扬,朱强,等.超声波辅助水酶法萃取葫芦籽油的研究[J].中国粮油学报,2012,27(10):53-56.