中西药联用对美人鱼发光杆菌的体外抑菌试验
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摘要
为了探讨中西药联用对美人鱼发光杆菌的体外抑菌效果,试验采用试管二倍稀释法分别测定2种西药(氟苯尼考、阿米卡星)和3种中药(女贞子、黄柏、夏枯草)对美人鱼发光杆菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),微量肉汤棋盘稀释法测定中西药联用对美人鱼发光杆菌的联合抑菌指数(FIC)。结果表明:这5种药物对美人鱼发光杆菌均有不同程度的抑制作用,且每种药物单独使用时以阿米卡星的抑菌效果最好。在6种药物组合中,氟苯尼考和黄柏组合及阿米卡星和黄柏组合在抑菌方面表现为相加作用;氟苯尼考和夏枯草组合及氟苯尼考和女贞子组合在抑菌方面表现为无关作用;阿米卡星和女贞子组合及阿米卡星和夏枯草组合在抑菌方面表现为颉颃作用。说明中西药联用对美人鱼发光杆菌具有一定程度的抑制作用。
引文
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[19]昌莉丽,李华坤,刘桂兰.中西药联用对鸭疫里默氏杆菌体外抑菌效果的研究[J].黑龙江畜牧兽医,2016(07下):137-139.
[20]马驿,陈钦旭,陈秀眉,等.鹅巴氏杆菌病病原分离鉴定及中西药联用抑菌试验[J].中兽医医药杂志,2011(2):46-49.
[21]廖广仁,赖伟华,黄孝榆.庆大霉素与3种中药联用对细菌释放内毒素的影响研究[J].中国药房,2006,17(24):1856-1857.
[22]佟延南,王德强,李芳远,等.防治花鳗鲡细菌性疾病的中西药筛选[J].安徽农业科学,2014,42(31):10971-10972.
[2]ANDREONI F,MAGNANI M. Photobacteriosis:prevention and diagnosis[J]. J Immunol Res,2014,2014:79(38)1-7.
[3]BARNES A C,DOS SANTOS N M,ELLIS A E. Update on bacterial vaccines:Photobacterium damselae subsp. piscicida[J].Dev Biol(Basel),2005,121:75-84.
[4]王小亮,徐立蒲,王姝,等.鱼源嗜水气单胞菌对氟苯尼考的耐药性分析[J].中国畜牧兽医,2014,41(6):216-220.
[5]DEL CASTILLO C S,HIKIMA J,JANG H B,et al. Comparative sequence analysis of a multidrug-resistant plasmid from Aeromonas hydrophila[J]. Antimicrob Agents Chemother,2013,57(1):120-129.
[6]聂鲡蓉,郑曙明,仇登高. 12种中药有效成分对3株水产致病菌体外抑菌试验[J].水生态学杂志,2010(6):141-144.
[7]胡金城,刘金兰,吕爱军,等.中草药在水产养殖中的应用[J].天津水产,2016(1):15-22.
[8]卢静,王振宁,陈锐,等.几种中药单体和抗生素对嗜水气单胞菌及温和气单胞菌的体外抑菌活性研究[J].水生生物学报,2013,37(6):1128-1132.
[9]李忠琴,关瑞章,汪黎虹,等.氟苯尼考与中药联用对养殖鳗鲡主要病原菌的体外抗菌活性分析[J].养殖与饲料,2013(3):13-17.
[10]昌莉丽,李华坤,刘桂兰.中西药联用对禽源大肠杆菌的体外抑菌效果研究[J].安徽农业科学,2014(16):5037-5038.
[11]陈晓利,彭彬,占爱思,等.黄鳝源嗜水气单胞菌的体外中药药敏试验[J].淡水渔业,2014,44(2):43-46.
[12]曲径.抗禽多杀性巴氏杆菌中药组方的筛选及颗粒剂的研制[D].雅安:四川农业大学,2015.
[13]丁月霞,黎梓葱,张秋玲,等. 13种中草药对禽源葡萄球菌体外抑菌试验[J].畜牧与饲料科学,2017,38(4):68-70.
[14]RIVAS A J,LEMOS M L,OSORIO C R. Photobacterium damselae subsp. damselae,a bacterium pathogenic for marine animals and humans[J]. Front Microbiol,2013,4:283.
[15]ALVAREZ J R,LAMBA S,DYER K Y,et al. An unusual case of urinary tract infection in a pregnant woman with Photobacterium damselae[J]. Infect Dis Obstet Gynecol,2006,2006:80682-80685.
[16]ALTER T,APPEL B,BARTELT E,et al. Vibrio infections from food and sea water. Introducing the “VibrioNet”[J]. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz,2011,54(11):1235-1240.
[17]VEZZULLI L,COLWELL R R,PRUZZO C. Ocean warming and spread of pathogenic vibrios in the aquatic environment[J]. Microb Ecol,2013,65(4):817-825.
[18]RIVAS A J,VON HOVEN G,NEUKIRCH C,et al. Phobalysin,a smallβ-pore-forming toxin of Photobacterium damselae subsp.damselae[J]. Infect Immun,2015,83(11):4335-4348.
[19]昌莉丽,李华坤,刘桂兰.中西药联用对鸭疫里默氏杆菌体外抑菌效果的研究[J].黑龙江畜牧兽医,2016(07下):137-139.
[20]马驿,陈钦旭,陈秀眉,等.鹅巴氏杆菌病病原分离鉴定及中西药联用抑菌试验[J].中兽医医药杂志,2011(2):46-49.
[21]廖广仁,赖伟华,黄孝榆.庆大霉素与3种中药联用对细菌释放内毒素的影响研究[J].中国药房,2006,17(24):1856-1857.
[22]佟延南,王德强,李芳远,等.防治花鳗鲡细菌性疾病的中西药筛选[J].安徽农业科学,2014,42(31):10971-10972.