HPLC法测定喜树不同部位喜树碱的含量
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摘要
采用高效液相色谱法测定喜树7个不同部位中喜树碱的含量.色谱柱为Diamonsil TM C18(4. 6×150mm,5μm);流动相为V(甲醇)∶V(水)(=55∶45);检测波长254 nm;柱温35℃;流速0. 8 m L·min-1.结果表明,种籽中喜树碱含量最高,嫩叶、嫩枝其次,喜树碱在0. 13~0. 24μg/m L范围内线性关系良好,平均回收率为99. 6%,RSD为1. 7%.本方法具有简单高效,灵敏度高,选择性强等优势,可用于测定喜树中喜树碱的含量.
HPLC is used to determine the camptothecin in seven parts of camptotheca acuminate decne. The separation was performed on Diamonsil TM C18( 4. 6 × 150 mm,5 μm) at 35℃; and the mobile phase was methanol and water( 55: 45),with the detection wavelength of 254 nm and the velocity of flow of 0. 8 m L·min-1. Results showed that the highest content of camptothecin appeared in the seed,followed by the tender leaf and twig. The linear range was 0.13 ~ 0. 24 μg/m L,the average recovery of camptothecin was 99. 6% and RSD was 1. 7%. The method was simple,high sensitivity,perfect precision and selectivity for determination of camptothecin in comptotheca acuminata decne.
HPLC is used to determine the camptothecin in seven parts of camptotheca acuminate decne. The separation was performed on Diamonsil TM C18( 4. 6 × 150 mm,5 μm) at 35℃; and the mobile phase was methanol and water( 55: 45),with the detection wavelength of 254 nm and the velocity of flow of 0. 8 m L·min-1. Results showed that the highest content of camptothecin appeared in the seed,followed by the tender leaf and twig. The linear range was 0.13 ~ 0. 24 μg/m L,the average recovery of camptothecin was 99. 6% and RSD was 1. 7%. The method was simple,high sensitivity,perfect precision and selectivity for determination of camptothecin in comptotheca acuminata decne.
引文
[1]李春杰,谷莹,韩应许,等.喜树碱及其衍生物的研究进展[J].中国药物化志,2003,13(5):306-310.
[2]冯乙巳,徐超,储玲玲,等.喜树碱衍生物的研究进展[J].合肥工业大学学报(自然科学报),2007,30(5):279-282.
[3]杨磊,李晓娟,赵春健.喜树生物碱在喜树中的分布[J].植物生理学通讯,2008,44(5):873-876.
[4] Wall M E,Wani M C,Cook C E,et al. Plant Antitum or Agents[J]. The isotation and structure of camptothecin a novel alkaloidal leukemia and inhibitor from camptotheca[J]. Jamer Chem Soc,1996,88(16):3888-3890.
[5] Hsiang Y H,Heribeng R,Hechts S,et al. Camptothecin induces protein-linked DNA breaks via mammalian DNA Topoisomerase[J]. Jbiol Chem Soc,1985,260(27):14873-14875.
[6]边清泉.喜树碱标样制备方法的研究[J].绵阳师范学院学报,2004,23(2):59-61.
[7]苏爱雪,周翔,罗娅君,等. HPLC法测定并比较喜树果、马比木中10-羟基喜树碱含量[J].产业与科技论坛,2014,5(13):78-79.
[8]秦庆芳,粟本超.广西不同地区喜树嫩叶中喜树碱含量测定[J].医药导报,2015,34(3):388-391.
[9]张玉红.喜树果实中喜树碱含量的产地差异及季节变化[J].东北林业大学学报,2002,30(6):44-47.
[10]周翔,林森,罗娅君,等.喜树果与马比木中喜树碱含量的比较[J].食品与发酵科技,2014,50(2):89-91.
[11]阎秀峰,王洋,于涛,等.喜树叶中喜树碱含量的高效液相色谱分析[J].分析测试学报,2002,21(2):15-17.
[12]李猛,黄意涵,李沁媛,等. HPLC法测定马比木中喜树碱的含量[J].绵阳师范学院学报,2012,29(2):68-70.
[2]冯乙巳,徐超,储玲玲,等.喜树碱衍生物的研究进展[J].合肥工业大学学报(自然科学报),2007,30(5):279-282.
[3]杨磊,李晓娟,赵春健.喜树生物碱在喜树中的分布[J].植物生理学通讯,2008,44(5):873-876.
[4] Wall M E,Wani M C,Cook C E,et al. Plant Antitum or Agents[J]. The isotation and structure of camptothecin a novel alkaloidal leukemia and inhibitor from camptotheca[J]. Jamer Chem Soc,1996,88(16):3888-3890.
[5] Hsiang Y H,Heribeng R,Hechts S,et al. Camptothecin induces protein-linked DNA breaks via mammalian DNA Topoisomerase[J]. Jbiol Chem Soc,1985,260(27):14873-14875.
[6]边清泉.喜树碱标样制备方法的研究[J].绵阳师范学院学报,2004,23(2):59-61.
[7]苏爱雪,周翔,罗娅君,等. HPLC法测定并比较喜树果、马比木中10-羟基喜树碱含量[J].产业与科技论坛,2014,5(13):78-79.
[8]秦庆芳,粟本超.广西不同地区喜树嫩叶中喜树碱含量测定[J].医药导报,2015,34(3):388-391.
[9]张玉红.喜树果实中喜树碱含量的产地差异及季节变化[J].东北林业大学学报,2002,30(6):44-47.
[10]周翔,林森,罗娅君,等.喜树果与马比木中喜树碱含量的比较[J].食品与发酵科技,2014,50(2):89-91.
[11]阎秀峰,王洋,于涛,等.喜树叶中喜树碱含量的高效液相色谱分析[J].分析测试学报,2002,21(2):15-17.
[12]李猛,黄意涵,李沁媛,等. HPLC法测定马比木中喜树碱的含量[J].绵阳师范学院学报,2012,29(2):68-70.