绞股蓝总黄酮通过抑制氧化应激反应抗心肌缺血保护作用的研究
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摘要
目的:研究绞股蓝总黄酮抗心肌缺血保护作用及其抗氧化应激反应研究。方法:40只大鼠随机分为假手术组、模型组、绞股蓝总黄酮组、生理盐水组,每组10只,假手术组只穿线不结扎,其他组大鼠进行心肌缺血再灌注损伤造模。造模24 h后,测定各组大鼠心肌梗死面积;测定血清心肌酶(LDH、CK、GOT)活力、氧化应激指标(MDA、SOD、GSH-Px和CAT)表达水平;测定心肌组织中氧化应激指标(MDA、SOD、GSH-Px和CAT)表达水平。结果:与假手术组相比,模型组心肌梗死面积显著增大(P <0. 05),血清LDH、CK、GOT活力显著升高(P <0. 05),血清和心肌组织中MDA水平显著升高(P <0. 01),SOD、CAT和GSH-Px活力显著下降(P <0. 01)。与模型组和生理盐水组相比,绞股蓝总黄酮组心肌梗死面积明显减小(P <0. 05),血清LDH、CK、GOT活力显著升高(P <0. 05),血清和心肌组织中MDA水平显著升高(P <0. 01),SOD、CAT和GSH-Px活力显著下降(P <0. 01)。结论:绞股蓝总黄酮可以显著改善缺血再灌注大鼠氧化应激反应,减少心肌梗死面积,起到保护心肌作用。
Objective:To study the protective effect of total flavones from Gynura divaricata against myocardial ischemia and its anti-oxidative stress response. Methods: Forty rats were randomly divided into sham operation group,model group,total gypenosides group and normal saline group,with 10 rats in each group. Sham-operated rats were not ligated by threading. The other rats were subjected to myocardial ischemia-reperfusion injury model. After 24 h of modeling,myocardial infarct size was determined in each group. The activities of serum myocardial enzymes( LDH,CK,GOT) and the expression of oxidative stress indicators( MDA,SOD,GSH-Px and CAT) expression levels were compared. Results:Compared with the sham operation group,the area ofmyocardial infarction in model group was significantly increased( P <0. 05);the activities of serum LDH,CK and GOT were significantly increased( P < 0. 05) and the levels of MDA in serum and myocardial tissue were significantly increased( P < 0. 01). The activities of SOD,CAT and GSH-Px were significantly decreased( P < 0. 01). Compared with the model group and the saline group,the myocardial infarct size in the total flavonoids of Gynostemma pentaphyllum decreased significantly( P < 0. 05); the activities of serum LDH,CK and GOT increased significantly( P < 0. 05) and MDA levels in serum and myocardium significantly increased( P < 0. 01).The activities of SOD,CAT and GSH-Px decreased significantly( P < 0. 01). Conclusion: The total flavonoids of Gynostemma can significantly improve the oxidative stress response of rats with ischemia-reperfusion,reduce the area of myocardial infarction and plays a role in protecting myocardium.
Objective:To study the protective effect of total flavones from Gynura divaricata against myocardial ischemia and its anti-oxidative stress response. Methods: Forty rats were randomly divided into sham operation group,model group,total gypenosides group and normal saline group,with 10 rats in each group. Sham-operated rats were not ligated by threading. The other rats were subjected to myocardial ischemia-reperfusion injury model. After 24 h of modeling,myocardial infarct size was determined in each group. The activities of serum myocardial enzymes( LDH,CK,GOT) and the expression of oxidative stress indicators( MDA,SOD,GSH-Px and CAT) expression levels were compared. Results:Compared with the sham operation group,the area ofmyocardial infarction in model group was significantly increased( P <0. 05);the activities of serum LDH,CK and GOT were significantly increased( P < 0. 05) and the levels of MDA in serum and myocardial tissue were significantly increased( P < 0. 01). The activities of SOD,CAT and GSH-Px were significantly decreased( P < 0. 01). Compared with the model group and the saline group,the myocardial infarct size in the total flavonoids of Gynostemma pentaphyllum decreased significantly( P < 0. 05); the activities of serum LDH,CK and GOT increased significantly( P < 0. 05) and MDA levels in serum and myocardium significantly increased( P < 0. 01).The activities of SOD,CAT and GSH-Px decreased significantly( P < 0. 01). Conclusion: The total flavonoids of Gynostemma can significantly improve the oxidative stress response of rats with ischemia-reperfusion,reduce the area of myocardial infarction and plays a role in protecting myocardium.
引文
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[25]周茹,何耀,和丽芬,等.丹参水提物对大鼠心肌缺血-再灌注损伤氧化应激的影响[J].中药药理与临床,2014,30(2):76-77.
[2]高仲录.自拟益肾健脾复脉法对心悸患者静息心率水平及心肌缺血症状的影响[J].四川中医,2016,34(12):70-73.
[3]张钊,利鹏婧,杨洋.绞股蓝研究综述[J].食品研究与开发,2011,32(11):193-196.
[4]李乐,高小利,丁宝兴.绞股蓝总黄酮对缺氧心肌细胞损伤标记物及细胞内游离Ca2+浓度的影响[J].浙江工业大学学报,2008,36(1):23-25.
[5]黄新宇,刘永林.白藜芦醇激活Nrf2/ARE信号通路降低心肌缺血再灌注损伤大鼠炎症和氧化应激[J].中华中医药学刊,2017,35(6):1516-1520.
[6]汤世民,吴咏梅,周雨亭,等.丹参酮IIA对心肌缺血再灌注损伤大鼠氧化应激的保护作用[J].中国医药导报,2017,14(4):8-11.
[7]刘征,冯其茂,董耀荣,等.通心方对慢性心肌缺血小猪冠脉侧支循环的研究[J].中华中医药学刊,2017,35(9):2356-2361.
[8]Thielmann M1,Kottenberg E,Kleinbongard P,et al. CardioProtective and prognosticeffects of remote ischaemic preconditioning in patientsundergoing coronary artery bypass surgery:a singlecentre randomised,double-blind,controlled trial[J]. Lancet,2013,382(9892):597-604.
[9]吕磊,郭华,张洁,等.糖原合酶激酶-3β在川芎嗪对心肌缺血再灌注损伤保护中的作用[J].东南大学学报(医学版),2016,35(6):836-840.
[10]曹旭丹,任春梅,和田田,等.水飞蓟宾对心肌缺血再灌注损伤大鼠心功能和氧化应激的影响[J].中国循征心血管医学杂志,2017,9(5):573-576.
[11]樊孝廉,舒毅,曾春平,等.~(99)Tc~m-MIBI门控心肌灌注显像评价2型糖尿病心功能的临床价值[J].标记免疫分析与临床,2017,24(6):614-616,620.
[12]Mangano DT,Tudor IC,Dietzel C,et al. The risk associated with aprotinin in cardiac surgery[J]. N Engl J Med,2006,354(4):353-365.
[13]刘锦源,司林杰.酒石酸布托啡诺预处理对小鼠心肌缺血再灌注损伤的保护作用及其机制[J].东南大学学报(医学版),2017,36(1):74-77.
[14]万芳,高应东.缺血修饰白蛋白的临床应用进展[J].标记免疫分析与临床,2017,24(7):823-826.
[15]Morita K. Surgical reoxygenation injury of the myocardium in cyanoticpatients:clinical relevance and therapeutic strategies by normoxicmanagement during cardiopulmonary bypass[J]. Gen Thorac Cardiovasc Surg,2012,60(9):549-556.
[16]杨海,梁宗夏,陈远东,等.缺血修饰清蛋白在冠心病心肌缺血早期的应用评价[J].检验医学与临床,2016,13(22):3239-3240.
[17]赵世杰,齐国先,田文,等.活体冷冻技术在评估小鼠心脏缺血再灌注损伤中的应用[J].现代医学,2016,44(4):445-449.
[18]Sánchez A,Calpena AC,Clares B. Evaluatingthe oxidative stress in inflammation:Role ofmelatonin[J]. Int J Mol Sci,2015,16(8):16981-7004.
[19]吕祥威,徐彤彤. 6-姜酚抑制氧化应激减轻大鼠心肌缺血/再灌注损伤[J].临床心血管病杂志,2017,33(6):575-579.
[20]陈香.运用动态心电图检测评价心肌缺血伴室性期前收缩起源部位的意义[J].检验医学与临床,2017,14(11):1586-1588.
[21]Guerin P,Bigot E,Patrice T. Evidence forantioxidants consumption in the coronary blood of patientswith an acute myocardial infarction[J]. J Thromb Thrombolysis,2013,35(1):41-47.
[22]邵莹,吴启南,周婧,等.竹叶黄酮对大鼠心肌缺血/再灌注损伤的保护作用[J].药理学通报,2013,29(2):241-247.
[23]许波华,许立.中药抗心肌缺血作用机制的研究进展[J].中国实验方剂学杂志,2011,17(15):265-269.
[24]乔钰惠,孟增慧,郭丽君,等.心肌缺血再灌注损伤的机制和治疗[J].基础医学与临床,2015,35(12):1666-1671.
[25]周茹,何耀,和丽芬,等.丹参水提物对大鼠心肌缺血-再灌注损伤氧化应激的影响[J].中药药理与临床,2014,30(2):76-77.