活性与非活性克雷伯氏菌对水中Pb~(2+)的吸附性能
|
摘要
针对从云南某矿山废水中分离出的1株克雷伯氏菌(Klebsiella sp.),研究其活性菌体(AK)和非活性菌体(IK)对水溶液中Pb~(2+)的吸附性能。研究了pH、吸附剂用量、反应时间和初始Pb~(2+)浓度、背景阴离子和共存阳离子对AK和IK吸附Pb~(2+)的影响,采用Zeta电位和FTIR分析进一步阐述2种吸附剂吸附Pb~(2+)的差异。在不同的实验条件下,AK对Pb~(2+)的吸附量均高于IK。AK和IK的吸附动力学均符合伪二级动力学模型,吸附等温线都能更好地使用Langmuir模型拟合。背景阴离子NO32-、Cl-和SO42-对AK和IK去除Pb~(2+)影响不大。Pb~(2+)、Zn~(2+)和Cd~(2+)3种重金属离子共存时,AK和IK对Pb~(2+)的吸附具有选择性。Zeta电位和FTIR分析表明,AK比IK具有更强的结合Pb~(2+)的能力,参与反应的基团主要是羟基、氨基和羧基。
A laboratory experiment was conducted for investigating Pb~(2+) adsorption properties of biomass of Klebsiella sp.,which was separated from the effluents from a Yunnan mine.Active Klebsiella sp.(AK) and inactive Klebsiella sp.(IK),prepared in laboratory,were applied as Pb~(2+) adsorbents;the effects of different parameters on Pb~(2+) removal performances of AK and IK,which included pH of the solution,the adsorbent amount,contact time,initial Pb~(2+) concentration in the solution,background anions and coexisting cations,were respectively studied.Zeta potential and Fourier transform infrared spectroscopy(FTIR) measurements were also used to study the difference between AK and IK in terms of Pb~(2+) adsorption.The experiment suggested that AK has greater Pb~(2+) adsorption capacity than that of IK under different experimental conditions;Pb~(2+) adsorption process by AK and IK could be described by pseudo-second-order kinetics model and Langmuir isotherm,respectively;and the presence of the background anions such as NO32-,Cl-and SO42-had little impacts on the removal of Pb~(2+) by AK and IK,however,when Pb~(2+) ,Zn~(2+) and Cd~(2+) co-existed,there appeared a sort of competitive adsorption but it barely affected removal of Pb~(2+) .Additionally,analysis of Zeta potential and FTIR indicated that the AK had greater affinity with Pb~(2+) than IK did,and hydroxyl,amine and carboxyl were the major groups participating in the adsorption process.
A laboratory experiment was conducted for investigating Pb~(2+) adsorption properties of biomass of Klebsiella sp.,which was separated from the effluents from a Yunnan mine.Active Klebsiella sp.(AK) and inactive Klebsiella sp.(IK),prepared in laboratory,were applied as Pb~(2+) adsorbents;the effects of different parameters on Pb~(2+) removal performances of AK and IK,which included pH of the solution,the adsorbent amount,contact time,initial Pb~(2+) concentration in the solution,background anions and coexisting cations,were respectively studied.Zeta potential and Fourier transform infrared spectroscopy(FTIR) measurements were also used to study the difference between AK and IK in terms of Pb~(2+) adsorption.The experiment suggested that AK has greater Pb~(2+) adsorption capacity than that of IK under different experimental conditions;Pb~(2+) adsorption process by AK and IK could be described by pseudo-second-order kinetics model and Langmuir isotherm,respectively;and the presence of the background anions such as NO32-,Cl-and SO42-had little impacts on the removal of Pb~(2+) by AK and IK,however,when Pb~(2+) ,Zn~(2+) and Cd~(2+) co-existed,there appeared a sort of competitive adsorption but it barely affected removal of Pb~(2+) .Additionally,analysis of Zeta potential and FTIR indicated that the AK had greater affinity with Pb~(2+) than IK did,and hydroxyl,amine and carboxyl were the major groups participating in the adsorption process.
引文
[1]Hasan H A,Abdullah S R S,Kofli N T,et al.Interaction of environmental factors on simultaneous biosorption of lead and manganese ions by locally isolated Bacillus cereus[J].Journal of Industrial&Engineering Chemistry,2016,37:295-305.
[2]Witek Krowiak A,Szafran R G,Modelski S.Biosorption of heavy metals from aqueous solutions onto peanut shell as a low-cost biosorbent[J].Desalination,2011,265(1/2/3):126-134.
[3]Wang L,Zhang J,Zhao R,et al.Adsorption of Pb(Ⅱ)on activated carbon prepared from Polygonum orientale Linn.:kinetics,isotherms,pH,and ionic strength studies[J].Bioresource Technology,2010,101(15):5808-5814.
[4]廖鹏飞,张武昌,穆巍巍,等.钝顶螺旋藻磁性生物吸附剂的制备及对铬(Ⅵ)的吸附性能[J].中南大学学报:自然科学版,2011,42(9):2551-2557.Liao Pengfei,Zhang Wuchang,Mu Weiwei,et al.Preparation and Cr(Ⅵ)biosorption properties of magnetic biosorbents of Spirulina platensis[J].Journal of Central South University:Science and Technology,2011,42(9):2551-2557.
[5]胡晓婧,藏婷婷,顾海东,等.平菇菌糠对废水中铜离子的生物吸附性能[J].环境科学,2014,35(2):669-677.Hu Xiaojing,Zang Tingting,Gu Haidong,et al.Biosorption characteristics of Cu2+by spent substrate of pleurotus oyster[J].Environmental Science,2014,35(2):669-677.
[6]Li W W,Yu H Q.Insight into the roles of microbial extracellular polymer substances in metal biosorption[J].Bioresource Technology,2014,160(2):15.
[7]Manzoor Q,Nadeem R,Iqbal M,et al.Organic acids pretreatment effect on Rosa bourbonia phyto-biomass for removal of Pb(Ⅱ)and Cu(Ⅱ)from aqueous media[J].Bioresource Technology,2013,132(2):446-452.
[8]Cerino Córdova F J,García León A M,Soto Regalado E,et al.Experimental design for the optimization of copper biosorption from aqueous solution by Aspergillus terreus[J].Journal of Environmental Management,2012,95(2):S77-S82.
[9]Das S,Mishra J,Das S K,et al.Investigation on mechanism of Cr(Ⅵ)reduction and removal by Bacillus amyloliquefaciens,a novel chromate tolerant bacterium isolated from chromite mine soil[J].Chemosphere,2014,96(2):112-121.
[10]Daneshvar E,Vazirzadeh A,Niazi A,et al.A comparative study of methylene blue biosorption using different modified brown,red and green macroalgae-effect of pretreatment[J].Chemical Engineering Journal,2016,307:435-446.
[11]Mu?±Oz A J,Esp?Nola F,Moya M,et al.Biosorption of Pb(Ⅱ)ions by Klebsiella sp.3S1 isolated from a wastewater treatment plant:kinetics and mechanisms studies[J].Biomed Research International,2015,2015(9):719060.
[12]Chakravarty R,Banerjee P C.Mechanism of cadmium binding on the cell wall of an acidophilic bacterium[J].Bioresource Technology,2012,108(108):176-183.
[13]?olak F,Atar N,Yaz?c?oglu D,et al.Biosorption of lead from aqueous solutions by Bacillus strains possessing heavy-metal resistance[J].Chemical Engineering Journal,2011,173(2):422-428.
[14]Masoumi F,Khadivinia E,Alidoust L,et al.Nickel and lead biosorption by Curtobacterium sp.FM01,an indigenous bacterium isolated from farmland soils of northeast Iran[J].Journal of Environmental Chemical Engineering,2016,4(1):950-957.
[15]Joo J H,Hassan S H A,Oh SE.Comparative study of biosorption of Zn2+by Pseudomonas aeruginosa and Bacillus cereus[J].International Biodeterioration&Biodegradation,2010,64(8):734-741.
[16]周旭东,李彩云,高鹏程,等.活体微藻吸附水体中Cd2+的性能特征[J].微生物学通报,2017,44(5):1182-1188.Zhou Xudong,Li Caiyun,Gao Pengcheng,et al.Adsorption of Cd2+in water by living microalgae[J].Microbiology China,2017,44(5):1182-1188.
[17]李进,冯冲凌,李科林,等.抗铅锌功能菌生长菌株和非活性菌体吸附Pb2+、Zn2+性能优化及机理分析[J].微生物学通报,2015,42(7):1224-1233.Li Jin,Feng Chongling,Li Kelin,et al.Biosorption of Pb(Ⅱ)and Zn(Ⅱ)by the growing strain/dry biomass of a resistant fungus:optimization and mechanism studies[J].Microbiology China,2015,42(7):1224-1233.
[18]Bhat S V,Melo J S,Chaugule BB,et al.Biosorption characteristics of uranium(Ⅵ)from aqueous medium onto Catenella repens,a red alga[J].Journal of Hazardous Materials,2008,158(2/3):628-635.
[19]Stojakovic D,Hrenovic J,Mazaj M,et al.On the zinc sorption by the Serbian natural clinoptilolite and the disinfecting ability and phosphate affinity of the exhausted sorbent[J].Journal of Hazardous Materials,2011,185(1):408-415.
[20]Huang F,Dang Z,Guo C L,et al.Biosorption of Cd(Ⅱ)by live and dead cells of Bacillus cereus RC-1 isolated from cadmium-contaminated soil[J].Colloids&Surfaces B-Biointerfaces,2013,107(4B):11-18.
[21]Reddy D H K,Lee S M.Three-dimensional porous spinel ferrite as an adsorbent for Pb(Ⅱ)removal from aqueous solutions[J].Industrial&Engineering Chemistry Research,2013,52(45):15789-15800.
[22]He C,Zhou Q,Duan Z,et al.One-step synthesis of aβ-cyclodextrin derivative and its performance for the removal of Pb(Ⅱ)from aqueous solutions[J].Research on Chemical Intermediates,2018:1-16.2011,185(1):408-415.
[23]邓莉萍.藻体对水环境中N、P及重金属Cu、Pb、Cd、Cr的吸附特征研究[D].青岛:中国科学院海洋研究所,2008.Deng Liping.Bioaccumulation and Biosorption of Nitrogen,Phosphorus and Heavy Metal Ions Cu2+,Pb2+,Cd2+and Cr6+[D].Qingdao:Institute of Oceanography,Chinese Academy of Sciences,2008.
[24]Hashim M A,Chu K H.Biosorption of cadmium by brown,green,and red seaweeds[J].Chemical Engineering Journal,2004,97(2):249-255.
[25]Matheickal J T,Yu Q.Biosorption of lead(Ⅱ)and copper(Ⅱ)from aqueous solutions by pre-treated biomass of Australian marine algae[J].Bioresource Technology,1999,69(3):223-229.
[26]Mahamadi C,Nharingo T.Competitive adsorption of Pb2+,Cd2+and Zn2+ions onto Eichhornia crassipes in binary and ternary systems[J].Bioresource Technology,2010,101(3):859-864.
[27]RemenárováL,Pipí?ka M,Horník M,et al.Biosorption of cadmium and zinc by activated sludge from single and binary solutions:mechanism,equilibrium and experimental design study[J].Journal of the Taiwan Institute of Chemical Engineers,2012,43(3):433-443.
[28]Zhu Y,Hu J,Wang J.Competitive adsorption of Pb(Ⅱ),Cu(Ⅱ)and Zn(Ⅱ)onto xanthate-modified magnetic chitosan[J].Journal of Hazardous Materials,2012,221/222(2):155-161.
[29]Ma X,Cui W,Yang L,et al.Efficient biosorption of lead(Ⅱ)and cadmium(Ⅱ)ions from aqueous solutions by functionalized cell with intracellular CaCO3mineral scaffolds[J].Bioresource Technology,2015,185:70-78.
[30]Zhang W,Meng L,Mu G,et al.A facile strategy for fabrication of nano-ZnO/yeast composites and their adsorption mechanism towards lead(Ⅱ)ions[J].Applied Surface Science,2016,378:196-206.
[31]信欣,姚力,崔钶,等.耐铅产絮克雷伯氏菌胞外聚合物EPS-07吸附水中Pb(Ⅱ)的特性[J].中国有色金属学报,2012(9):2667-2675.Xin Xin,Yao Li,Cui Ke,et al.Sorption characteristics of Pb(Ⅱ)from aqueous solution by extracellular polymeric substance(EPS-07)of lead-resistant and producing flocculant strain Klebsiella pneumonia[J].The Chinese Journal of Nonferrous Metals,2012(9):2667-2675.
[2]Witek Krowiak A,Szafran R G,Modelski S.Biosorption of heavy metals from aqueous solutions onto peanut shell as a low-cost biosorbent[J].Desalination,2011,265(1/2/3):126-134.
[3]Wang L,Zhang J,Zhao R,et al.Adsorption of Pb(Ⅱ)on activated carbon prepared from Polygonum orientale Linn.:kinetics,isotherms,pH,and ionic strength studies[J].Bioresource Technology,2010,101(15):5808-5814.
[4]廖鹏飞,张武昌,穆巍巍,等.钝顶螺旋藻磁性生物吸附剂的制备及对铬(Ⅵ)的吸附性能[J].中南大学学报:自然科学版,2011,42(9):2551-2557.Liao Pengfei,Zhang Wuchang,Mu Weiwei,et al.Preparation and Cr(Ⅵ)biosorption properties of magnetic biosorbents of Spirulina platensis[J].Journal of Central South University:Science and Technology,2011,42(9):2551-2557.
[5]胡晓婧,藏婷婷,顾海东,等.平菇菌糠对废水中铜离子的生物吸附性能[J].环境科学,2014,35(2):669-677.Hu Xiaojing,Zang Tingting,Gu Haidong,et al.Biosorption characteristics of Cu2+by spent substrate of pleurotus oyster[J].Environmental Science,2014,35(2):669-677.
[6]Li W W,Yu H Q.Insight into the roles of microbial extracellular polymer substances in metal biosorption[J].Bioresource Technology,2014,160(2):15.
[7]Manzoor Q,Nadeem R,Iqbal M,et al.Organic acids pretreatment effect on Rosa bourbonia phyto-biomass for removal of Pb(Ⅱ)and Cu(Ⅱ)from aqueous media[J].Bioresource Technology,2013,132(2):446-452.
[8]Cerino Córdova F J,García León A M,Soto Regalado E,et al.Experimental design for the optimization of copper biosorption from aqueous solution by Aspergillus terreus[J].Journal of Environmental Management,2012,95(2):S77-S82.
[9]Das S,Mishra J,Das S K,et al.Investigation on mechanism of Cr(Ⅵ)reduction and removal by Bacillus amyloliquefaciens,a novel chromate tolerant bacterium isolated from chromite mine soil[J].Chemosphere,2014,96(2):112-121.
[10]Daneshvar E,Vazirzadeh A,Niazi A,et al.A comparative study of methylene blue biosorption using different modified brown,red and green macroalgae-effect of pretreatment[J].Chemical Engineering Journal,2016,307:435-446.
[11]Mu?±Oz A J,Esp?Nola F,Moya M,et al.Biosorption of Pb(Ⅱ)ions by Klebsiella sp.3S1 isolated from a wastewater treatment plant:kinetics and mechanisms studies[J].Biomed Research International,2015,2015(9):719060.
[12]Chakravarty R,Banerjee P C.Mechanism of cadmium binding on the cell wall of an acidophilic bacterium[J].Bioresource Technology,2012,108(108):176-183.
[13]?olak F,Atar N,Yaz?c?oglu D,et al.Biosorption of lead from aqueous solutions by Bacillus strains possessing heavy-metal resistance[J].Chemical Engineering Journal,2011,173(2):422-428.
[14]Masoumi F,Khadivinia E,Alidoust L,et al.Nickel and lead biosorption by Curtobacterium sp.FM01,an indigenous bacterium isolated from farmland soils of northeast Iran[J].Journal of Environmental Chemical Engineering,2016,4(1):950-957.
[15]Joo J H,Hassan S H A,Oh SE.Comparative study of biosorption of Zn2+by Pseudomonas aeruginosa and Bacillus cereus[J].International Biodeterioration&Biodegradation,2010,64(8):734-741.
[16]周旭东,李彩云,高鹏程,等.活体微藻吸附水体中Cd2+的性能特征[J].微生物学通报,2017,44(5):1182-1188.Zhou Xudong,Li Caiyun,Gao Pengcheng,et al.Adsorption of Cd2+in water by living microalgae[J].Microbiology China,2017,44(5):1182-1188.
[17]李进,冯冲凌,李科林,等.抗铅锌功能菌生长菌株和非活性菌体吸附Pb2+、Zn2+性能优化及机理分析[J].微生物学通报,2015,42(7):1224-1233.Li Jin,Feng Chongling,Li Kelin,et al.Biosorption of Pb(Ⅱ)and Zn(Ⅱ)by the growing strain/dry biomass of a resistant fungus:optimization and mechanism studies[J].Microbiology China,2015,42(7):1224-1233.
[18]Bhat S V,Melo J S,Chaugule BB,et al.Biosorption characteristics of uranium(Ⅵ)from aqueous medium onto Catenella repens,a red alga[J].Journal of Hazardous Materials,2008,158(2/3):628-635.
[19]Stojakovic D,Hrenovic J,Mazaj M,et al.On the zinc sorption by the Serbian natural clinoptilolite and the disinfecting ability and phosphate affinity of the exhausted sorbent[J].Journal of Hazardous Materials,2011,185(1):408-415.
[20]Huang F,Dang Z,Guo C L,et al.Biosorption of Cd(Ⅱ)by live and dead cells of Bacillus cereus RC-1 isolated from cadmium-contaminated soil[J].Colloids&Surfaces B-Biointerfaces,2013,107(4B):11-18.
[21]Reddy D H K,Lee S M.Three-dimensional porous spinel ferrite as an adsorbent for Pb(Ⅱ)removal from aqueous solutions[J].Industrial&Engineering Chemistry Research,2013,52(45):15789-15800.
[22]He C,Zhou Q,Duan Z,et al.One-step synthesis of aβ-cyclodextrin derivative and its performance for the removal of Pb(Ⅱ)from aqueous solutions[J].Research on Chemical Intermediates,2018:1-16.2011,185(1):408-415.
[23]邓莉萍.藻体对水环境中N、P及重金属Cu、Pb、Cd、Cr的吸附特征研究[D].青岛:中国科学院海洋研究所,2008.Deng Liping.Bioaccumulation and Biosorption of Nitrogen,Phosphorus and Heavy Metal Ions Cu2+,Pb2+,Cd2+and Cr6+[D].Qingdao:Institute of Oceanography,Chinese Academy of Sciences,2008.
[24]Hashim M A,Chu K H.Biosorption of cadmium by brown,green,and red seaweeds[J].Chemical Engineering Journal,2004,97(2):249-255.
[25]Matheickal J T,Yu Q.Biosorption of lead(Ⅱ)and copper(Ⅱ)from aqueous solutions by pre-treated biomass of Australian marine algae[J].Bioresource Technology,1999,69(3):223-229.
[26]Mahamadi C,Nharingo T.Competitive adsorption of Pb2+,Cd2+and Zn2+ions onto Eichhornia crassipes in binary and ternary systems[J].Bioresource Technology,2010,101(3):859-864.
[27]RemenárováL,Pipí?ka M,Horník M,et al.Biosorption of cadmium and zinc by activated sludge from single and binary solutions:mechanism,equilibrium and experimental design study[J].Journal of the Taiwan Institute of Chemical Engineers,2012,43(3):433-443.
[28]Zhu Y,Hu J,Wang J.Competitive adsorption of Pb(Ⅱ),Cu(Ⅱ)and Zn(Ⅱ)onto xanthate-modified magnetic chitosan[J].Journal of Hazardous Materials,2012,221/222(2):155-161.
[29]Ma X,Cui W,Yang L,et al.Efficient biosorption of lead(Ⅱ)and cadmium(Ⅱ)ions from aqueous solutions by functionalized cell with intracellular CaCO3mineral scaffolds[J].Bioresource Technology,2015,185:70-78.
[30]Zhang W,Meng L,Mu G,et al.A facile strategy for fabrication of nano-ZnO/yeast composites and their adsorption mechanism towards lead(Ⅱ)ions[J].Applied Surface Science,2016,378:196-206.
[31]信欣,姚力,崔钶,等.耐铅产絮克雷伯氏菌胞外聚合物EPS-07吸附水中Pb(Ⅱ)的特性[J].中国有色金属学报,2012(9):2667-2675.Xin Xin,Yao Li,Cui Ke,et al.Sorption characteristics of Pb(Ⅱ)from aqueous solution by extracellular polymeric substance(EPS-07)of lead-resistant and producing flocculant strain Klebsiella pneumonia[J].The Chinese Journal of Nonferrous Metals,2012(9):2667-2675.