霉菌毒素分解酶枯草杆菌制剂对日粮含AFB1的艾维茵肉鸡的解毒效果研究
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摘要
为研究在含有微量黄曲霉毒素B1 (AFB1)日粮中添加霉菌毒素分解酶枯草杆菌制剂对肉鸡生长性能、免疫器官指数和抗氧化能力的影响,本研究将120羽7日龄艾维茵肉鸡随机分成4组,每组3个重复。A组饲喂基础日粮,B、C、D组在基础日粮中分别添加50μg/kg AFB1、50μg/kg AFB1+0.1%枯草芽孢杆菌Pab02制剂和50μg/kg AFB1+0.1%霉菌毒素分解酶枯草杆菌制剂,饲喂28 d后测定肉鸡的生长性能、免疫器官指数、抗氧化能力及AFB1在肝脏和肌肉的残留量。结果显示:试验期间,除B组鸡出现精神沉郁和6.67%死亡率外,其余3组鸡均未出现病理症状和死亡现象;净增重D>A>C>B组(p>0.05),料重比B>C>D>A组(p>0.05);脾脏指数A>D>C>B组,并且A、D、C组极显著高于B组(p<0.01);B组血清和肝脏中的超氧化物歧化酶(SOD)活性和总抗氧化能力(T-AOC)显著或极显著低于A、D组(p<0.05或p<0.01),丙二醛(MDA)含量极显著高于A、C、D组(p<0.01);各组肉鸡肝脏和肌肉中黄曲霉毒素的残留量A To investigate the effect of the Bacillus subtilis and mycotoxins enzyme compound preparation on growth performance, immune organ index and antioxidant ability of broilers fed with diets containing Aflatoxin B1(AFB1), a total of 1207-day-old Avian broilers were randomly divided into 4 groups with 3 replicates in each group. Group A fed with basal diet, group B, C and D were fed with basal diet supplemented with 50μg/kg AFB1, 50μg/kg AFB1+0.1% Bacillus subtilis Pab02 preparation,and 50μg/kg AFB1+0.1% Bacillus subtilis and mycotoxins enzyme compound preparation, respectively. The growth performance,immune organ index, antioxidant ability, and AFB1 residues in the liver and muscle of broilers were detected after 28 days. The results showed that broilers in group B became depressed and lethargic, as well as the mortality rate in this group was 6.67%during the trial period, while the broilers in the remaining groups did not show these pathological symptoms and broiler deaths; the net weight gain of broilers in four groups was different, D>A>C>B(p>0.05), the feed conversion ratio was ordered as B>C>D >A(p>0.05); the four groups spleen index in turn was: A>D>C>B, and the first three groups were significantly higher than that of group B(p<0.01); the activity of SOD and T-AOC in serum and liver of group B were extremely lower than that in group A and D(p<0.05 or p<0.01), and the MDA content of group B was extremely significant higher than that in group A, C and D(p<0.01);the AFB1 residues in liver and muscle of four groups increased gradually from A, D, C to B in order, and group A, C and D showed a significant decrease compared with group B(p<0.01). These results suggest that Bacillus subtilis Pab02, Bacillus subtilis and mycotoxins enzyme compound preparation could not only decrease AFB1 residues in broilers, but also improving the following symptoms induced by AFB1, such as the stunted growth, the mal development of immune organ, the decreased antioxidant ability and so on. Furthermore, the antidotal effect of the Bacillus subtilis and mycotoxins enzyme compound preparation is better than Bacillus subtilis Pab02. This study provides the experimental basis for the application of Bacillus subtilis and mycotoxins enzyme compound preparation in production.
引文
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[19]李国富,何一凡,吴清坛,陈顺金.血清SOD、GSH-Px、T-AOC及MDA的测定对肺炎诊断的临床意义[J].现代临床医学生物工程学杂志,2003,(03):196-197.
[20]曹红,尹逊慧,陈善林,等.黄曲霉毒素解毒酶对岭南黄肉仔鸡日粮中黄曲霉毒素B_1解毒效果的研究[J].动物营养学报,2010, 22(2):424-430.
[2] Abdel-Aziem S H, Hassan A M, Abdel-Wahhab M A. Dietary supplementation with whey protein and ginseng extract counteracts oxidative stress and DNA damage in rats fed an aflatoxin-contaminated diet[J]. Mutati Res, 2011, 723(1):65-71.
[3]李超波.黄曲霉毒素B1降解菌的分离鉴定及其降解特性[D].南昌:南昌大学,2012.
[4]江涛,马良,张宇昊,等.光谱法研究黄曲霉毒素B1与人血清白蛋白的结合反应[J].分析化学,2016, 44(1):54-60.
[5]计成,赵丽红,马秋刚,等.黄曲霉毒素生物降解的研究及前景展望[J].动物营养学报,2010,31(2):6-9.
[6]宋文静,Latifa Rizka Fidaroin,齐德生,等.微生物对肉鸭肝脏及肌肉中黄曲霉毒素B1残留的影响[J].饲料工业,2013,34(08):8-11.
[7]王晓阁.枯草芽孢杆菌研究进展与展望[J].中山大学研究生学刊(自然科学.医学版),2012,(3):14-23.
[8]潘康成,杨金龙,王振华,等.枯草芽孢杆菌制剂对肉鸡生长、胴体和鸡肉品质的影响[J].中国饲料,2005,(20):11-14.
[9]张盼,李培武,万霞,等.枯草芽孢杆菌NattO3的筛选及其降解黄曲霉毒素B1的初步研究[J].中国油料作物学报,2015, 37(2):234-239.
[10]潘康成,张新华,张钧利,等.霉菌毒素分解酶对育肥猪生产性能及肝功能的影响[J].饲料工业,2016,37(11):39-41.
[11]薛秀青.~(99)Tc-MDP对D-半乳糖衰老模型小鼠抗氧化作用的实验研究[D].苏州:苏州大学,2011.
[12]尹逊慧,陈善林,曹红,等.日粮添加黄曲霉毒素解毒酶制剂对黄羽肉鸡生产性能、血清生化指标和毒素残留的影响[J].中国家禽,2010,33(2):29-33.
[13]石达友,李鹏飞,郭铭生,等.不同剂量黄曲霉毒素B1对雏鸭生长性能的影响[J].中国兽医杂志,2010,46(4):22-23.
[14] Zuo Rui-yu, Chang Juan, Yin Qing-qiang, et al. Effect of the combined probiotics with aflatoxin B1-degrading enzyme onaflatoxin detoxification, broiler production performance and hepatic enzyme gene expression[J]. Food Chemic Toxicol, 2013,59(8):470-475.
[15]邓庆庆.乳酸菌对饲喂黄曲霉毒素B1污染日粮肉鸡生产性能和免疫功能的影响[D].洛阳:河南科技大学,2016.
[16]余东游,毛翔飞,秦艳,等.枯草芽孢杆菌对肉鸡生长性能及其抗氧化和免疫功能的影响[J].中国畜牧杂志,2010,46(3):22-25.
[17] Thaxton J P, Tung H T, Hamilton P B. Immunosuppression in Chickens by Aflatoxin[J]. Poultry Science, 1974, 53(2):721-725.
[18]王荣梅,苏荣胜,潘家强,等.黄曲霉毒素对动物免疫及抗氧化能力的影响[J].饲料研究,2010,(2):54-56.
[19]李国富,何一凡,吴清坛,陈顺金.血清SOD、GSH-Px、T-AOC及MDA的测定对肺炎诊断的临床意义[J].现代临床医学生物工程学杂志,2003,(03):196-197.
[20]曹红,尹逊慧,陈善林,等.黄曲霉毒素解毒酶对岭南黄肉仔鸡日粮中黄曲霉毒素B_1解毒效果的研究[J].动物营养学报,2010, 22(2):424-430.