小麦材料CH7034中成株期抗白粉病QTL定位
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摘要
小麦生长过程中常因白粉病侵扰而造成产量和品质损失,发掘新抗源、鉴定新位点是小麦品种抗性改良的基础。CH7034是本实验室选育的一份抗白粉病材料。本研究利用芯片技术对CH7034与感病品种SY95-71的重组自交系(recombinant inbred lines, RIL)群体进行扫描,结合3年成株抗白粉病鉴定数据,鉴定出5个QTL-Pm,之后开发SSR标记对主效位点QPm.sac-2B.1 (表型变异解释率为30.6%～33.6%)进行图谱加密,进一步将其定位在小麦2B染色体长臂683 034 934～703 889 622基因组区段内,侧翼标记为X2BL-NRM12和X2BL-NRM17。本研究结果为小麦抗病育种提供了新抗源和可用于PCR检测的常规分子标记。
During the grow th of wheat, yield and quality losses are often caused by powdery mildew. Finding resistant sources and identifying resistant loci are the basis for resistance improvement of wheat varieties. CH7034 is a powdery mildew resistant material bred in our laboratory. In this study, five QTL-Pm were identified by SNP-chip technology in the RIL(Recombinant inbred lines) population crossed by CH7034 and susceptible variety SY95-71 with identification data of adult plant resistance to powdery mildew for three years. Then SSR markers were developed to construct the more densely linkage map of the major QTL-Pm, QPm.sac-2 B.1, with a phenotypic variation interpretation rate of 30.6%～33.6%. Finally, QPm.sac-2 B.1 was located in the 683 034 934～703 889 622 genomic section on the long arm of wheat chromosome 2 B with flanking marker X2 BL-NRM12 and X2 BL-NRM17.The results could provide new resistance source and conventional PCR-detected molecular markers for wheat resistance breeding.
During the grow th of wheat, yield and quality losses are often caused by powdery mildew. Finding resistant sources and identifying resistant loci are the basis for resistance improvement of wheat varieties. CH7034 is a powdery mildew resistant material bred in our laboratory. In this study, five QTL-Pm were identified by SNP-chip technology in the RIL(Recombinant inbred lines) population crossed by CH7034 and susceptible variety SY95-71 with identification data of adult plant resistance to powdery mildew for three years. Then SSR markers were developed to construct the more densely linkage map of the major QTL-Pm, QPm.sac-2 B.1, with a phenotypic variation interpretation rate of 30.6%～33.6%. Finally, QPm.sac-2 B.1 was located in the 683 034 934～703 889 622 genomic section on the long arm of wheat chromosome 2 B with flanking marker X2 BL-NRM12 and X2 BL-NRM17.The results could provide new resistance source and conventional PCR-detected molecular markers for wheat resistance breeding.
引文
Bougot Y.,Lemoine J.,Pavoine M.T.,Guyomar'ch H.,Gautier V.,Muranty H.,and Barloy D.,2006,A major QTL effect controlling resistance to powdery mildew in winter wheat at the adult plant stage,Plant Breed.,125(6):550-556
Gao H.Y.,Yang X.W.,He D.X.,Niu J.S.,Wang C.Y.,and Wu Y.,2015,Effects of wheat powdery mildew on grain yield and quality and its prevention and control measures,Shandong Nongye Kexue(Shandong Agricultural Sciences),47(10):139-144(高红云,杨习文,贺德先,牛吉山,王晨阳,吴寅,2015,小麦白粉病对籽粒产量和品质的影响及防控途径,山东农业科学,47(10):139-144)
Griffey C.,Das M.,and Stromberg E.,1993,Effectiveness of adult-plant resistance in reducing grain yield loss to powdery mildew in winter wheat,Plant Dis.,77(6):618-622
He R.L.,Chang Z.J.,Liu J.X.,Zhan H.X.,Zhang X.J.,and Dong C.L.,2008,Chromosomal location of powdery mildew resistance gene in Thinopyrum ponticum-derived wheat germplasm line CH7034,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),6(2):251-256(贺润丽,畅志坚,刘建霞,詹海仙,张晓军,董春林,2008,源于长穗偃麦草的小麦新品系CH7034抗白粉病基因的染色体定位,分子植物育种,6(2):251-256)
He Z.H.,Xia X.C.,Luo J.,Xin Z.Y.,Kong X.Y.,Jing R.L.,Wu Z.L.,and Li X.P.,2006,Trend analysis of international wheat breeding,Mailei Zuowu Xuebao(Journal of Triticeae Crops),26(2):154-156(何中虎,夏先春,罗晶,辛志勇,孔秀英,景蕊莲,吴振录,李杏普,2006,国际小麦育种研究趋势分析,麦类作物学报,26(2):154-156)
Hill-Ambroz K.L.,Brown-Guedira G.L.,and Fellers J.P.,2002,Modified rapid DNA extraction protocol for high throughput microsatellite analysis in wheat,Crop Sci.,42(6):2088-2091
Huang X.Q.,Hsam S.L.K.,and Zeller F.J.,1997,Identification of powdery mildew resistance genes in common wheat(Triticum aestivum L.em Thell.).IX.cultivars,land races and breeding lines grown in China,Plant Breed.,116(3):233-238
Lan C.X.,Ni X.W.,Yan J.,Zhang Y.,Xia X.C.,Chen X.M.,and He Z.H.,2010,Quantitative trait loci mapping of adult-plant resistance to powdery mildew in Chinese wheat cultivar Lumai 21,Mol.Breed.,25(4):615-622
Li J.B.,Qiao L.Y.,Li X.,Zhang X.J.,Zhan H.X.,Guo H.J.,Ren Y.K.,and Chang Z.J.,2015,Molecular mapping of powdery mildew resistance gene PmCH7124 in a putative wheat-Thinopyrum intermedium introgression line,Zuowu Xuebao(Acta Agronomica Sinica),41(1):49-56(李建波,乔麟轶,李欣,张晓军,詹海仙,郭慧娟,任永康,畅志坚,2015,小麦-中间偃麦草渗入系抗白粉病基因PmCH7124的分子定位,作物学报,41(1):49-56)
Liang S.S.,Suenaga K.,He Z.H.,Wang Z.L.,Liu H.Y.,Wang D.S.,Singh R.P.,Sourdille P.,and Xia X.C.,2006,Quantitative trait loci mapping for adult-plant resistance to powdery mildew in bread wheat,Phytopathology,96(7):784-789
Line R.F.,and Chen X.M.,1995,Success in breeding for and managing durable resistance to wheat rusts,Plant Dis.,79(12):1254-1255
Liu S.X.,Griffey C.A.,and Saghai-Maroof M.A.,2001,Identification of molecular markers associated with adult plant resistance to powdery mildew in common wheat cultivar Massey,Crop Sci.,41(4):1268-1275
Qiao L.Y.,Zhang X.J.,Li X.,Zhang L.,Zheng J.,and Chang Z.J.,2017,Development of NBS-related microsatellite(NRM)markers in hexaploid wheat,Euphytica,213(11):256
Qin B.,Cao A.,Wang H.,Chen T.,You F.M.,Liu Y.,Ji J.,Liu D.,Chen P.,and Wang X.E.,2011,Collinearity-based marker mining for the fine mapping of Pm6,a powdery mildew resistance gene in wheat,Theor.Appl.Genet.,123(2):207-218
Sun H.G.,Hu J.H.,Song W.,Qiu D.,Cui L.,Wu P.P.,Zhang H.J.,Liu H.W.,Yang L.,Qu Y.F.,Li Y.H.,Li T.,Cheng W.,Zhou Y.,Liu Z.Y.,Li J.T.,and Li H.J.,2018,Pm61:a re cessive gene for resistance to powdery mildew in wheat landrace Xuxusanyuehuang identified by comparative genomics analysis,Theor.Appl.Genet.,131(10):2085-2097
Tucker D.M.,Griffey C.A.,Liu S.,Brown-Guedira G.,Marshall D.S.,and Saghai-Maroof M.A.,2007,Confirmation of three quantitative trait loci conferring adult plant resistance to powdery mildew in two winter wheat populations,Euphytica,155(1-2):1-13
Yang L.J.,Zeng F.S.,Gong S.J.,Shi W.Q.,Zhang X.J.,Wang H.,Xiang L.B.,and Yu D.Z.,2013,Evaluation of resistance to powdery mildew in 68 Chinese major wheat cultivars and postulation of their resistance genes,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),46(16):3354-3368(杨立军,曾凡松,龚双军,史文琦,张学江,汪华,向礼波,喻大昭,2013,68个主推小麦品种的白粉病抗性分析及基因推导,中国农业科学,46(16):3354-3368)
Zeller F.J.,and Hsam S.L.,1996,Chromosomal location of a gene suppressing powdery mildew resistance genes Pm8 and Pm17 in common wheat(Triticum aestivum L.em.Thell.),Theor.Appl.Genet.,93(1-2):38-40
Zhan H.X.,Li G.R.,Zhang X.J.,Li X.,Guo H.J.,Gong W.P.,Jia J.Q.,Qiao L.Y.,Ren Y.K.,Yang Z.J.,and Chang Z.J.,2014,Chromosomal location and comparative genomics analysis of powdery mildew resistance gene Pm51 in a putative wheat-Thinopyrum ponticum introgression line,PLoS One,9(11):e113455
Zhao Z.H.,Sun H.G.,Song W.,Lu M.,Huang J.,Wu L.F.,Wang X.M.,and Li H.J.,2013,Genetic analysis and detection of the gene MlLX99 on chromosome 2BL conferring resistance to powdery mildew in the wheat cultivar Liangxing 99,Theor.Appl.Genet.,126(12):3081-3089
Zhu Z.D.,Zhou R.H.,Kong X.Y.,Dong Y.C.,and Jia J.Z.,2005,Microsatellite markers linked to 2 powdery mildew resistance genes introgressed from Triticum carthlicum accession PS5 into common wheat,Genome,48(4):585-590
Gao H.Y.,Yang X.W.,He D.X.,Niu J.S.,Wang C.Y.,and Wu Y.,2015,Effects of wheat powdery mildew on grain yield and quality and its prevention and control measures,Shandong Nongye Kexue(Shandong Agricultural Sciences),47(10):139-144(高红云,杨习文,贺德先,牛吉山,王晨阳,吴寅,2015,小麦白粉病对籽粒产量和品质的影响及防控途径,山东农业科学,47(10):139-144)
Griffey C.,Das M.,and Stromberg E.,1993,Effectiveness of adult-plant resistance in reducing grain yield loss to powdery mildew in winter wheat,Plant Dis.,77(6):618-622
He R.L.,Chang Z.J.,Liu J.X.,Zhan H.X.,Zhang X.J.,and Dong C.L.,2008,Chromosomal location of powdery mildew resistance gene in Thinopyrum ponticum-derived wheat germplasm line CH7034,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),6(2):251-256(贺润丽,畅志坚,刘建霞,詹海仙,张晓军,董春林,2008,源于长穗偃麦草的小麦新品系CH7034抗白粉病基因的染色体定位,分子植物育种,6(2):251-256)
He Z.H.,Xia X.C.,Luo J.,Xin Z.Y.,Kong X.Y.,Jing R.L.,Wu Z.L.,and Li X.P.,2006,Trend analysis of international wheat breeding,Mailei Zuowu Xuebao(Journal of Triticeae Crops),26(2):154-156(何中虎,夏先春,罗晶,辛志勇,孔秀英,景蕊莲,吴振录,李杏普,2006,国际小麦育种研究趋势分析,麦类作物学报,26(2):154-156)
Hill-Ambroz K.L.,Brown-Guedira G.L.,and Fellers J.P.,2002,Modified rapid DNA extraction protocol for high throughput microsatellite analysis in wheat,Crop Sci.,42(6):2088-2091
Huang X.Q.,Hsam S.L.K.,and Zeller F.J.,1997,Identification of powdery mildew resistance genes in common wheat(Triticum aestivum L.em Thell.).IX.cultivars,land races and breeding lines grown in China,Plant Breed.,116(3):233-238
Lan C.X.,Ni X.W.,Yan J.,Zhang Y.,Xia X.C.,Chen X.M.,and He Z.H.,2010,Quantitative trait loci mapping of adult-plant resistance to powdery mildew in Chinese wheat cultivar Lumai 21,Mol.Breed.,25(4):615-622
Li J.B.,Qiao L.Y.,Li X.,Zhang X.J.,Zhan H.X.,Guo H.J.,Ren Y.K.,and Chang Z.J.,2015,Molecular mapping of powdery mildew resistance gene PmCH7124 in a putative wheat-Thinopyrum intermedium introgression line,Zuowu Xuebao(Acta Agronomica Sinica),41(1):49-56(李建波,乔麟轶,李欣,张晓军,詹海仙,郭慧娟,任永康,畅志坚,2015,小麦-中间偃麦草渗入系抗白粉病基因PmCH7124的分子定位,作物学报,41(1):49-56)
Liang S.S.,Suenaga K.,He Z.H.,Wang Z.L.,Liu H.Y.,Wang D.S.,Singh R.P.,Sourdille P.,and Xia X.C.,2006,Quantitative trait loci mapping for adult-plant resistance to powdery mildew in bread wheat,Phytopathology,96(7):784-789
Line R.F.,and Chen X.M.,1995,Success in breeding for and managing durable resistance to wheat rusts,Plant Dis.,79(12):1254-1255
Liu S.X.,Griffey C.A.,and Saghai-Maroof M.A.,2001,Identification of molecular markers associated with adult plant resistance to powdery mildew in common wheat cultivar Massey,Crop Sci.,41(4):1268-1275
Qiao L.Y.,Zhang X.J.,Li X.,Zhang L.,Zheng J.,and Chang Z.J.,2017,Development of NBS-related microsatellite(NRM)markers in hexaploid wheat,Euphytica,213(11):256
Qin B.,Cao A.,Wang H.,Chen T.,You F.M.,Liu Y.,Ji J.,Liu D.,Chen P.,and Wang X.E.,2011,Collinearity-based marker mining for the fine mapping of Pm6,a powdery mildew resistance gene in wheat,Theor.Appl.Genet.,123(2):207-218
Sun H.G.,Hu J.H.,Song W.,Qiu D.,Cui L.,Wu P.P.,Zhang H.J.,Liu H.W.,Yang L.,Qu Y.F.,Li Y.H.,Li T.,Cheng W.,Zhou Y.,Liu Z.Y.,Li J.T.,and Li H.J.,2018,Pm61:a re cessive gene for resistance to powdery mildew in wheat landrace Xuxusanyuehuang identified by comparative genomics analysis,Theor.Appl.Genet.,131(10):2085-2097
Tucker D.M.,Griffey C.A.,Liu S.,Brown-Guedira G.,Marshall D.S.,and Saghai-Maroof M.A.,2007,Confirmation of three quantitative trait loci conferring adult plant resistance to powdery mildew in two winter wheat populations,Euphytica,155(1-2):1-13
Yang L.J.,Zeng F.S.,Gong S.J.,Shi W.Q.,Zhang X.J.,Wang H.,Xiang L.B.,and Yu D.Z.,2013,Evaluation of resistance to powdery mildew in 68 Chinese major wheat cultivars and postulation of their resistance genes,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),46(16):3354-3368(杨立军,曾凡松,龚双军,史文琦,张学江,汪华,向礼波,喻大昭,2013,68个主推小麦品种的白粉病抗性分析及基因推导,中国农业科学,46(16):3354-3368)
Zeller F.J.,and Hsam S.L.,1996,Chromosomal location of a gene suppressing powdery mildew resistance genes Pm8 and Pm17 in common wheat(Triticum aestivum L.em.Thell.),Theor.Appl.Genet.,93(1-2):38-40
Zhan H.X.,Li G.R.,Zhang X.J.,Li X.,Guo H.J.,Gong W.P.,Jia J.Q.,Qiao L.Y.,Ren Y.K.,Yang Z.J.,and Chang Z.J.,2014,Chromosomal location and comparative genomics analysis of powdery mildew resistance gene Pm51 in a putative wheat-Thinopyrum ponticum introgression line,PLoS One,9(11):e113455
Zhao Z.H.,Sun H.G.,Song W.,Lu M.,Huang J.,Wu L.F.,Wang X.M.,and Li H.J.,2013,Genetic analysis and detection of the gene MlLX99 on chromosome 2BL conferring resistance to powdery mildew in the wheat cultivar Liangxing 99,Theor.Appl.Genet.,126(12):3081-3089
Zhu Z.D.,Zhou R.H.,Kong X.Y.,Dong Y.C.,and Jia J.Z.,2005,Microsatellite markers linked to 2 powdery mildew resistance genes introgressed from Triticum carthlicum accession PS5 into common wheat,Genome,48(4):585-590