溢油胁迫下海洋微藻脂肪酸合成过程中碳稳定同位素分馏效应
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摘要
为了研究溢油对海洋生态系统中海洋微藻生长的影响,本文探讨了在不同含量燃料油180号水溶性成分(WAF)胁迫下纤细角毛藻脂肪酸碳稳定同位素比值(δ13C)的变化。结果表明,在燃料油WAF胁迫下,微藻生长过程中脂肪酸合成速率均受到不同程度的影响,尤其是不饱和脂肪酸16∶1和18∶4的δ13C值与油浓度(WAF)成大致的线性关系,这说明燃料油的毒害作用不仅使海洋微藻类不饱和脂肪酸合成过程受到影响,同时引起脂肪酸合成过程发生碳稳定同位素分馏效应。因此,部分不饱和脂肪酸如16∶1等可作为溢油对海洋微藻类胁迫程度评价的生物标志物。
In order to realize the effects of oil spill on marine microalgae growth,we studied the carbon stable isotope composition( δ13C) of Chaetoceros gracilis under the stress of different content of water accommodated fraction( WAF) of No. 180 fuel oil. The result shows that the rate of fatty acid synthesis are affected by different degree of the stress of WAF in the process of microalgae growth,especially there are approximately linear relationships between theδ13C values of unsaturated fatty acids( 16∶ 1 and 18∶ 4) and WAF,which may result from the kinetic stable isotope fractionation effect. Therefore parts of unsaturated fatty acids such as 16 ∶ 1 may be used as an alternative biomarker for estimating the pollution degree of oil spills.
In order to realize the effects of oil spill on marine microalgae growth,we studied the carbon stable isotope composition( δ13C) of Chaetoceros gracilis under the stress of different content of water accommodated fraction( WAF) of No. 180 fuel oil. The result shows that the rate of fatty acid synthesis are affected by different degree of the stress of WAF in the process of microalgae growth,especially there are approximately linear relationships between theδ13C values of unsaturated fatty acids( 16∶ 1 and 18∶ 4) and WAF,which may result from the kinetic stable isotope fractionation effect. Therefore parts of unsaturated fatty acids such as 16 ∶ 1 may be used as an alternative biomarker for estimating the pollution degree of oil spills.
引文
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[2]GAUR J P,KUMAR H D.Growth response of four microalgae to three crude oils and a furnace oil[J].Environmental Pollution Series A:Ecological and Biological,1981,25(1):77-85.
[3]GAMILA H A,IBRAHIMM B M.Algalboassay for evaluating the role of algae in bioremediation of crude oil:Iisolat edstrains[J].Bulletin of Environmental Contamination and Toxicology,2004,73(5):883-889.
[4]RIMET F,ECTOR L,DOHET A,et al.Impacts of fluoranthene on diatom assemblages and frustul emorphology in indoor microcosms[J].Vie Et Milieulife and Environment,2004,54(2/3):145-156.
[5]白志鹏,张利文,朱坦,等.稳定同位素在环境科学研究中的应用进展[J].同位素,2007,20(1):57-64.
[6]曹福亮,王欢利,郁万文,等.高等植物脂肪酸去饱和酶及编码基因研究进展[J].南京林业大学学报(自然科学版),2012,36(2):125-132.
[7]曾硕士,江黎明,元冬娟.脂肪酸去饱和酶的研究进展[J].生命科学,2008,20(5):816-820.
[8]陈刚,肖慧,唐学玺.3种海洋赤潮微藻蛋白质和核酸合成动态对芘胁迫的响应[J].海洋环境科学,2008,27(4):320-322.
[9]王修林,杨茹君,祝陈坚.石油烃污染物存在下旋链角毛藻生长的粒度效应初步研究[J].中国海洋大学学报,2004,34(5):849-853.
[10]张蕾,王修林,韩秀荣,等.石油烃污染物对海洋浮游植物生长的影响——实验与模型[J].青岛海洋大学学报,2002,32(5):804-810.
[11]王悠,唐学玺,李永祺,等.低浓度蒽对两种海洋微藻生长的兴奋效应[J].应用生态学报,2002,13(3):343-346.
[12]张聿柏.石油烃对海洋微藻的毒性效应及其机理研究[D].青岛:中国海洋大学,2013.