高效液相色谱法同时测定炒防风中升麻素苷和5-O-甲基维斯阿米醇苷含量
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摘要
目的建立同时测定炒防风中升麻素苷和5-O-甲基维斯阿米醇苷含量的高效液相色谱法。方法色谱柱为Rigol-C_(18)柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(40∶60),流速为1.0 mL/min,进样量为5μL,检测波长为254 nm,柱温为35℃。结果可在20 min内完成对升麻素苷和5-O-甲基维斯阿米醇苷的色谱分析,升麻素苷和5-O-甲基维斯阿米醇苷的质量浓度线性范围分别为0.0116~0.2920 g/L(r=0.9994)和0.0114~0.2850 g/L(r=0.9994),平均回收率分别为95.82%和107.70%,RSD分别为2.92%和2.45(n=6)。结论该法方便、快捷、准确、简便、重复性好,可用于炒防风的质量控制。
Objective To establish an HPLC method for simultaneous determination of prim-O-glucosylcimifugin and 5-O-methylvisammioside in fried Radix Saposhnikovia divaricata. Methods The Rigol-C_(18) column( 250 mm × 4. 6 mm,5 μm) was adopted,the mobile phase was methanol-water( 40 ∶ 60),the flow rate was 1. 0 mL/min,the sample size was 5 μL,the detection wavelength was set at 254 nm,and the column temperature was 35 ℃. Results The chromatographic analysis of prim-O-glucosylcimifugin and 5-O-methylvisammioside were completed within 20 min. Prim-O-glucosylcimifugin and 5-O-methylvisammioside showed good linear relationship within the ranges of 0. 0116-0. 2920 g/L( r = 0. 9994) and 0. 0114-0. 2850 g/L( r = 0. 9994), the average recoveries were 95. 82% and 107. 70% and RSDs were 2. 92% and 2. 45%( n = 6). Conclusion The method is convenient,quick,accurate,simple and reproducible,which can be used for quality control of fried Radix Saposhnikovia divaricata.
Objective To establish an HPLC method for simultaneous determination of prim-O-glucosylcimifugin and 5-O-methylvisammioside in fried Radix Saposhnikovia divaricata. Methods The Rigol-C_(18) column( 250 mm × 4. 6 mm,5 μm) was adopted,the mobile phase was methanol-water( 40 ∶ 60),the flow rate was 1. 0 mL/min,the sample size was 5 μL,the detection wavelength was set at 254 nm,and the column temperature was 35 ℃. Results The chromatographic analysis of prim-O-glucosylcimifugin and 5-O-methylvisammioside were completed within 20 min. Prim-O-glucosylcimifugin and 5-O-methylvisammioside showed good linear relationship within the ranges of 0. 0116-0. 2920 g/L( r = 0. 9994) and 0. 0114-0. 2850 g/L( r = 0. 9994), the average recoveries were 95. 82% and 107. 70% and RSDs were 2. 92% and 2. 45%( n = 6). Conclusion The method is convenient,quick,accurate,simple and reproducible,which can be used for quality control of fried Radix Saposhnikovia divaricata.
引文
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[15]赵博,杨鑫宝,杨秀伟,等.HPLC法同时测定防风中6个主要成分的含量[J].药物分析杂志,2013,33(3):382-387.
[2]丁安荣,王奇志,李淑莉,等.防风化学成分的研究[J].中草药,1987,18(6):7-9.
[3]赵博,杨鑫宝,张连学,等.防风灵——防风中1个新的香豆素类化合物[J].中国中药杂志,2010,35(11):1418-1420.
[4]赵博,杨鑫宝,张连学,等.防风化学成分的研究[J].中国中药杂志,2010,35(12):1569-1572.
[5]夏伦祝,彭代银,汪永忠,等.三种方法提取防风挥发油的气质联用成分分析[J].安徽医药,2009,13(8):885-886.
[6]陈勇,李晓如,曾笑,等.气相色谱-质谱和化学计量学解析法分析防风挥发油成分[J].中国医院药学杂志,2008,28(6):500-502.
[7]严云丽,张华,金高娃,等.防风挥发油化学成分分析比较[J].世界科学技术-中医药现代化,2009,11(3):400-406.
[8]李洪峰,于英,金星海,等.不同产地防风中挥发油成分分析[J].黑龙江水专学报,2007,34(4):101-104.
[9]王松柏,秦雪梅,刘焕蓉,等.防风多糖化学成分的研究[J].化学研究,2008,19(2):66-68.
[10]薛宝文,李文,李丽,等.防风色原酮甙类成分的药理活性研究[J].中国中药杂志,2000,25(5):297-299.
[11]高鸿霞,邵世和,王国庆.中药防风的研究进展[J].井冈山医专学报,2004,11(4):12-14.
[12]杨波,曹玲,王喜军.CO2超临界萃取物的药效学研究[J].中医药学报,2006,34(1):14-15.
[13]胡紫艳,冯振斌,蒋健.HPLC测定不同产地防风中4种有效成分的含量[J].中国执业药师,2016,13(11):31-34.
[14]邵留英,喻志斌,瞿发林.不同产地防风中有效成分含量比较[J].海峡药学,2013,25(2):52-54.
[15]赵博,杨鑫宝,杨秀伟,等.HPLC法同时测定防风中6个主要成分的含量[J].药物分析杂志,2013,33(3):382-387.