健脾复方对结直肠癌细胞增殖及侵袭迁移的影响
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摘要
目的探讨健脾复方对结直肠癌细胞增殖、侵袭和迁移及MALAT1表达的影响。方法通过CCK-8法检测健脾复方对LoVo细胞增殖的影响;根据CCK-8法检测结果,设立空白对照组和健脾复方高、中、低浓度组,然后采用Transwell实验检测健脾复方对LoVo细胞侵袭和迁移能力的影响;采用RT-PCR检测健脾复方对LoVo细胞长链非编码RNA MALAT1表达的影响。结果 LoVo细胞的生长抑制率与健脾复方浓度呈正相关;侵袭实验显示与对照组相比,到达侵袭小室底部的LoVo细胞数量随着健脾复方浓度增高而减少;Transwell迁移实验显示健脾复方能够抑制LoVo细胞的迁移能力,并且随着健脾复方浓度的增高,其迁移能力显著降低。健脾复方亦能对MALAT1表达起抑制作用。结论健脾复方可抑制结直肠癌细胞增殖、侵袭和转移并降低MALAT1表达,其抑制LoVo细胞侵袭转移的机制可能与降低MALAT1表达有关。
Objective This paper intents to investigate the influence of Jian Pi compound on the proliferation,invasion,migration,and the MALAT1 expression of colorectal cancer cells.MethodsThe CCK-8 was chosen to count the proliferation state of LoVo cells after treated with Jian Pi compound.The control group,the high,the medium,and the low concentration groups of Jian Pi compound were established according to the results obtained by CCK-8.Then,the migration and invasion of LoVo cells were measured by the Transwell.Besides the expression of long noncoding,MALAT1 in the LoVo cells was tested by RT-PCR.Results The growth inhibition rate of LoVo cells was positively correlated with the concentration of Jian Pi compound.Compared with the control group,the number of LoVo cells which reached the bottom of Transwells decreased significantly with the increase of the concentration of Jian Pi compound in Transwell invasion testes.Besides,the Transwel migration test also indicated that the migration ability of LoVo was inhibited by Jian Pi compound,and the migration ability of LoVo notablely declined as the concentration rises.In addition the expression of MALTA1 was also restrained by Jian Pi compound.Conclusions Jian Pi compound could inhibit the proliferation,invasion,migration of colorectal cancer cells.The inhibition mechanism may be connected with the depression of MALAT1.
Objective This paper intents to investigate the influence of Jian Pi compound on the proliferation,invasion,migration,and the MALAT1 expression of colorectal cancer cells.MethodsThe CCK-8 was chosen to count the proliferation state of LoVo cells after treated with Jian Pi compound.The control group,the high,the medium,and the low concentration groups of Jian Pi compound were established according to the results obtained by CCK-8.Then,the migration and invasion of LoVo cells were measured by the Transwell.Besides the expression of long noncoding,MALAT1 in the LoVo cells was tested by RT-PCR.Results The growth inhibition rate of LoVo cells was positively correlated with the concentration of Jian Pi compound.Compared with the control group,the number of LoVo cells which reached the bottom of Transwells decreased significantly with the increase of the concentration of Jian Pi compound in Transwell invasion testes.Besides,the Transwel migration test also indicated that the migration ability of LoVo was inhibited by Jian Pi compound,and the migration ability of LoVo notablely declined as the concentration rises.In addition the expression of MALTA1 was also restrained by Jian Pi compound.Conclusions Jian Pi compound could inhibit the proliferation,invasion,migration of colorectal cancer cells.The inhibition mechanism may be connected with the depression of MALAT1.
引文
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15 黄劲龙,沈建箴.长链非编码RNA MALAT-1在肿瘤中的作用及其机制研究进展[J].中国肿瘤临床,2016,43:161-165.
16 杨孜欢,冯杏芝,方乐堃,等.长链非编码RNA MALAT1调控Rac1b表达与结直肠癌侵袭和转移的关系[J].中国病理生理杂志,2015,31:1417-1421.
17 朱栋良,尹小平,王芳元.长链非编码RNA-MALAT1对结直肠癌细胞介导的血管形成的影响[J].中国普外基础与临床杂志,2015,22:60-63.
18 王雅娟,胡洁,赵海燕,等.EMT与非EMT细胞在大肠癌侵袭转移中的作用[J].基础医学与临床,2014,34:1165-1170.
19 任建琳,刘宣,陈文婷,等.丹参酮ⅡA通过COX-2/PGE2-β-catenin通路抑制人肠癌细胞VEGF表达[A].中华中医药学会.发挥中医优势,注重转化医学——2013年全国中医肿瘤学术年会论文汇编[C].中华中医药学会,2013:6.
20 徐胜美,马红梅,郭东.MMP-7在大肠癌中的表达与其侵袭转移的关系[J].实用癌症杂志,2009,24:570-572.
21 陈文婷,石齐,侯风刚,等.健脾复方联合化疗治疗大肠癌患者疗效指标系统评价[J].辽宁中医药大学学报,2014,16:146-148.
22 陈文婷,刘宣,任建琳,等.基于聚类分析法的大肠癌术后患者中医用药规律研究[J].中华中医药学刊,2016,34:101-104.
23 刘宣,周利红,隋华,等.健脾解毒方对大肠癌细胞COX-2/β-catenin/MMP-7信号通路的影响[J].上海中医药杂志,2016,50:65-69.
24 任建琳,吕祥,陈文婷,等.健脾复方通过Wnt/β-catenin信号通路抑制裸鼠人大肠癌转移[J].中国医药科学,2014,4:23-25,28.
2 周晓东,吕农华.大肠癌的流行病学研究现状[J].现代消化及介入诊疗,2006,11:149-151.
3 陈万青,郑荣寿,张思维,等.2012年中国恶性肿瘤发病和死亡分析[J].中国肿瘤,2016,25:1-8.
4 李洲宇,梁启廉,周圆,等.1290例大肠癌临床流行病学特征的比较分析[J].医学研究杂志,2012,41:73-76.
5 Hsu CW,King TM,Chang MC,et al.Factors that influence survival in colorectal cancer with synchronous distant metastasis[J].J Chin Med Assoc,2012,75:370-375.
6 Watson AJ,Collins PD.Colon cancer:a civilization disorder[J].Dig Dis,2011,29:222-228.
7 Court F,Baniol M,Hagegen H,et al.Long-range chromatin interactions at the mouse Igf2/H19 locus reveal a novel paternally expressed long non-coding RNA[J].Nucleic Acids Res,2011,39:5893-5906.
8 常建兰,李祖国,王晓燕,等.p53、malat1、ki-67和β-catenin基因mRNA检测在大肠癌分子诊断中的意义[J].世界华人消化杂志,2008,16:3849-3854.
9 田智,刘亮华,廖江涛.长链非编码核糖核酸Malat-1、p21和GAS5在大肠癌组织中的表达及其诊断价值[J].华中科技大学学报:医学版,2017,46:10-14.
10 Lai MC,Yang Z,Zhou L,et al.Long non-coding RNA MALAT-1 overexpression predicts tumor recurrence of hepatocellular carcinoma after liver transplantation[J].Med Oncol,2012,29:1810-1816.
11 Fan Y,Shen B,Tan M,et al.TGF-beta-induced upregulation of malat1 promotes bladder cancer metastasis by associating with suz12[J].Clin Cancer Res,2014,20:1531-1541.
12 Ji Q,Liu X,Fu X,et al.Resveratrol inhibits invasion and metastasis of colorectal cancer cells via MALAT1 mediated wnt/b-catenin signal pathway[J].PLoS One,2013,8:e78700.
13 季青,刘宣,周利红,等.健脾解毒方通过抑制MALAT1介导的β-catenin信号通路抗大肠癌侵袭转移[J].中华中医药杂志,2015,30:3877-3880.
14 靖琳,任建琳,季青,等.长链非编码RNA调控结直肠癌侵袭与转移机制的研究进展[J].国际消化病杂志,2015,35:334-337.
15 黄劲龙,沈建箴.长链非编码RNA MALAT-1在肿瘤中的作用及其机制研究进展[J].中国肿瘤临床,2016,43:161-165.
16 杨孜欢,冯杏芝,方乐堃,等.长链非编码RNA MALAT1调控Rac1b表达与结直肠癌侵袭和转移的关系[J].中国病理生理杂志,2015,31:1417-1421.
17 朱栋良,尹小平,王芳元.长链非编码RNA-MALAT1对结直肠癌细胞介导的血管形成的影响[J].中国普外基础与临床杂志,2015,22:60-63.
18 王雅娟,胡洁,赵海燕,等.EMT与非EMT细胞在大肠癌侵袭转移中的作用[J].基础医学与临床,2014,34:1165-1170.
19 任建琳,刘宣,陈文婷,等.丹参酮ⅡA通过COX-2/PGE2-β-catenin通路抑制人肠癌细胞VEGF表达[A].中华中医药学会.发挥中医优势,注重转化医学——2013年全国中医肿瘤学术年会论文汇编[C].中华中医药学会,2013:6.
20 徐胜美,马红梅,郭东.MMP-7在大肠癌中的表达与其侵袭转移的关系[J].实用癌症杂志,2009,24:570-572.
21 陈文婷,石齐,侯风刚,等.健脾复方联合化疗治疗大肠癌患者疗效指标系统评价[J].辽宁中医药大学学报,2014,16:146-148.
22 陈文婷,刘宣,任建琳,等.基于聚类分析法的大肠癌术后患者中医用药规律研究[J].中华中医药学刊,2016,34:101-104.
23 刘宣,周利红,隋华,等.健脾解毒方对大肠癌细胞COX-2/β-catenin/MMP-7信号通路的影响[J].上海中医药杂志,2016,50:65-69.
24 任建琳,吕祥,陈文婷,等.健脾复方通过Wnt/β-catenin信号通路抑制裸鼠人大肠癌转移[J].中国医药科学,2014,4:23-25,28.