白藜芦醇对小鼠淋巴细胞分泌细胞因子的影响
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摘要
为了探讨白藜芦醇的免疫增强作用。分离小鼠脾淋巴细胞,设空白对照组、阳性对照组及10、20、40和80μg/mL白藜芦醇组,采用ELISA法检测白藜芦醇对淋巴细胞分泌IL-3、IL-4、IL-12和IFN-γ细胞因子的影响。结果,白藜芦醇能诱导脾淋巴细胞IL-3、IL-4、IL-12和IFN-γ细胞因子的分泌。表明白藜芦醇可通过促进淋巴细胞细胞因子的分泌,来提高淋巴细胞的免疫功能。
To set up a blank control group, a positive control group, 10, 20, 40, and 80 ug/mL of resveratrol, and use ELISA to detect the effects of resveratrol on the lymphocytes to produce il-3, il-4, il-12, and IFN-gamma cytokines. As a result, resveratrol can induce the spleen lym‐phocytes IL-3, IL-4, IL-12 and IFN-gamma the secretion of cytokines. It is suggest that resvera‐trol can enhance lymphocyte immunity by promoting that secretion of cytokine in lymphocytes.
To set up a blank control group, a positive control group, 10, 20, 40, and 80 ug/mL of resveratrol, and use ELISA to detect the effects of resveratrol on the lymphocytes to produce il-3, il-4, il-12, and IFN-gamma cytokines. As a result, resveratrol can induce the spleen lym‐phocytes IL-3, IL-4, IL-12 and IFN-gamma the secretion of cytokines. It is suggest that resvera‐trol can enhance lymphocyte immunity by promoting that secretion of cytokine in lymphocytes.
引文
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[19]曹喆燚,吴沐芸,王嘉毅,等.红酒中白藜芦醇对辐射损伤小鼠免疫系统的保护作用[A].第十届全国免疫学学术大会汇编[C].北京:第十届全国免疫学学术大会,2015,88.
[2]Albert S,Horbach R,Deising H B,et al.Syn‐thesis and antimicrobial activity of(E)stil‐bene derivatives[J].Bioorganic&Medicinal Chemistry,2011,19(17):5155.
[3]Hu L,Cao D,Li Y,et al.Resveratrol sensi‐tized leukemia stem cell-like KG-1a cells to cytokine-induced killer cells-mediated cy‐tolysis through NKG2D ligands and TRAIL re‐ceptors[J].Cancer Biology&Therapy,2012,13(7):516-526.
[4]Zhang S,Cao H J,Davis F B,et al.Oestrogen inhibits resveratrol-induced post-transla‐tional modification of p53 and apoptosis in breast cancer cells[J].British Journal of Cancer,2004,91(1):178.
[5]冯永红,周文亮.低剂量白藜芦醇增强小鼠免疫反应[J].中国药理学报:英文版,2002(10):31-35.
[6]Lee K,Lee J H,Ryu S Y,et al.Stilbenes re‐duce Staphylococcus aureus hemolysis,biofilm formation,and virulence[J].Foodborne Pathogens&Disease,2014,11(9):710.
[7]李敬双,孟显华,刘天明,等.华蟾毒精对小鼠免疫细胞活性的影响[J].现代畜牧兽医,2014,(6):12-15.
[8]Xu Y Y,Chu F J,Wu Y P,et al.Effects of housefly maggots extracts on the prolifera‐tion and the CD4+/CD8+of mouser splenic lym‐phocytes in vitro[J].Guangdong Medical Journal,2013.
[9]王萌,韩嘉祺,张凤,等.桔梗皂苷D对小鼠脾淋巴细胞免疫调节活性的研究[J].中国兽医科学,2018,48(1):93-100.
[10]倪湾,李敬双,于洋,等.洋葱槲皮素对脂多糖诱导的小鼠腹腔巨噬细胞炎症反应抑制作用[J].食品工业科技,2017,23(3):284-288.
[11]Zhao N,Xu X X,Zhang Y J,et al.Compara‐tive study on the effects of lactoferricins from different sources on proliferation and cytokine secretion of spleen lymphocytes in mice[J].Modern Food Science&Technology,2015.
[12]Rosarosa J M,Graciaaznárez F J,Hodges E,et al.Deep sequencing of target linkage as‐say-identified regions in familial breast cancer:methods,analysis pipeline and trou‐bleshooting[J].Plos One,2010,5(4):e9976.
[13]Obleukhova I A,Kurilin V V,Goncharov M A,et al.Effect of mature dendritic cells primed with autologous tumor antigens from patients with epithelial ovarian cancer on stimulation of the cytotoxic immune response in culture of mononuclear cells[J].Bulletin of Experimental Biology and Medicine,2013,156(1):161-164.
[14]Kawamata A,Ito D,Odani T,et al.Thalido‐mide suppresses melanoma growth by activat‐ing natural killer cells in mice[J].Oncolo‐gy Reports,2006,16(6):1231-1236.
[15]Baird J R,Fox B A,Sanders K L,et al.Avirulent Toxoplasma gondii generates thera‐peutic antitumor immunity by reversing immu‐nosuppression in the ovarian cancer microen‐vironment[J].Cancer Research,2013,73(13):3842.
[16]ShinIchi Inoue,Mamoru Niikura,Shoichiro Mineo,et al.Roles of IFN-γandγδTCells in Protective Immunity Against BloodStage Malaria[J].Frontiers in Immunology,2013,4:258.
[17]Habijanic J,Berovic M,Boh B,et al.Sub‐merged cultivation of Ganoderma lucidum and the effects of its polysaccharides on the production of human cytokines TNF-α,IL-12,IFN-γ,IL-2,IL-4,IL-10 and IL-17[J].New Biotechnology,2015,32(1):85-95.
[18]雷宇鹏,曾振国,胡德泉.白藜芦醇对脂多糖诱导的巨噬细胞极化改变的影响[J].重庆医学,2015(19):2593-2595.
[19]曹喆燚,吴沐芸,王嘉毅,等.红酒中白藜芦醇对辐射损伤小鼠免疫系统的保护作用[A].第十届全国免疫学学术大会汇编[C].北京:第十届全国免疫学学术大会,2015,88.