代谢组学技术及其在药学研究领域的应用
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摘要
代谢组学是近年来兴起的一门学科,其研究对象主要是能反映生物体所处环境的生物体液、生物标本、细胞提取物、细胞培养液和组织,并利用核磁共振和质谱等技术进行分析。在药学研究领域已成为研究的热点。对代谢组学的概况、分析技术及其近些年在药学研究领域中的应用进行了综述,并对代谢组学这一研究方法的优缺点进行小结。
Metabonomics is a new science and technology developed in recent years. Major research objects of metabonomics are biological fluids which could reflect the environment of organisms,biological specimen,cell extracts,cell culture fluid and tissue,while NMR and MS are used as analysis methods. Metabonomics has been a research hotspot in the field of pharmacy. This paper reviews the analytical methods and the applications of metabolomics and summarize the advantages and disadvantages of metabolomics.
Metabonomics is a new science and technology developed in recent years. Major research objects of metabonomics are biological fluids which could reflect the environment of organisms,biological specimen,cell extracts,cell culture fluid and tissue,while NMR and MS are used as analysis methods. Metabonomics has been a research hotspot in the field of pharmacy. This paper reviews the analytical methods and the applications of metabolomics and summarize the advantages and disadvantages of metabolomics.
引文
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[6]梁强,郭晓辉,周蓓莉,等.代谢指纹分析及其在食品科学中的应用[J].食品与发酵工业,2011,37(10):134-136.
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[15]王勇,江芝婷,梁琼麟,等.代谢组学法研究三聚氰胺对儿童尿液代谢的影响[J].高等学校化学学报,2010,31(1):57-60.
[16]Kaddurah-Daouk R,Mc Evoy J,Baillie R A,et al.Metabolomic mapping of atypical antipsychotic effects inschizophrenia[J].Mol Psychiatry,2007,12(10):934-45.
[17]皮子凤,门丽慧,张静,等.五味子治疗大鼠糖尿病肾病作用机制的血清代谢组学研究[J].分析化学,2015,43(2):169-175.
[18]Clayton TA,Lindon JC,Cloarec O,et al.Pharmacometabonomic phenotyping and personalized drug treatment[J].Nature,2006,440(7087):1073-1077.
[19]曹蓓,阿基业,王广基,等.代谢组学在临床研究中的应用及进展[J].生命科学,2010,22(8):761-771.
[20]吕天,牟红元,冯江江,等.中药柴胡总皂苷急性肝毒性的代谢组学研究[J].化学研究与应用,2013,25(6):789-792.
[21]吴宏伟,李洪梅,唐力英,等.代谢组学方法研究姜黄根茎及块根次生代谢产物表达差异[J].分析化学研究报告,2012,40(5):713-717.
[22]Yongli Hua,Wenxin Xue,Man Zhang,et al.Metabonomics study on the hepatoprotective effect of polysaccharides from different preparations of Angelica sinensis[J].Journal of Ethnopharmacology,2014,151(2014)1090-1099.
[23]德洛,罗尚华,周林,等.代谢组学研究在藏医药理论及临床诊疗现代研究中的应用前景分析[Z].全国藏医药学术交流与适宜技术培训会(五藏区会议),甘肃合作市,2012.
[24]帕它木·莫合买提,哈木拉提·吾甫尔.代谢组学与维吾尔医现代研究[J].中国民族医药杂志,2008,1(1):63-65.
[2]Nicholson J K,Connelly J,Lindon J C,et al.Metabonomics:a platform for studying drug toxicity and gene function[J].Nature Reviews Drug Discovery,2002,1(2):153-161.
[3]Lindon J C,Holmes E,Nicholson J K.So what’s the deal with metabonomics?[J].Anal Chem,2003,75(17):384A-391A.
[4]German J,Bauman D,Burrin D,et a1.Metabolomies in the opening decade of the 21 st Century:building the roads to individualized health[J].J Nutr,2004,134(10):2729-2732.
[5]Fiehn O,Kopka J,Dormann P,et al.Metabolite profiling for plant functional genonmics[J].Nat Biotechnol,2000,18(11):1157-1161.
[6]梁强,郭晓辉,周蓓莉,等.代谢指纹分析及其在食品科学中的应用[J].食品与发酵工业,2011,37(10):134-136.
[7]Khan M T H,Ather A.Metabolomics-systematic studies of the metabolic profiling[J].Advances in Phytomedicine,2006,22(7):411-419.
[8]Dunn W B,Ellis D I.Metabolomics:current analytical platforms and methodologies[J].Trends in Anal Chem,2005,24(4):285-294.
[9]刘姗姗,陶金忠,赵兴绪,等.代谢组学分析技术及其在生殖领域中的应用[J].动物医学进展,2014,35(3):109-115.
[10]Dunn W B,Ellis D I.Metabolomics:current analytical platforms and methodologies[J].Tr AC Trends in Analy Chem,2005,24(4):285-294.
[11]Gellppi E.Contributions of liquid chromatography-mass spectrometry to“highlights”of biomedical research[J].J Chromatogra A,2003,1000(1):567-581.
[12]张瑞兴,刘舒,皮子凤,等.汞离子对细胞代谢通路影响的代谢组学[J].高等学校化学学报,2014,35(6):1146-1151.
[13]黄瑛.代谢组学在药物毒理学中的应用[J].药学实践杂志,2009,3(27):165-167.
[14]Slim R M,Roberstson D G,Ajbassam M,et al.Effect of dexamethasone on the metabonomics profile associated with phosphodiesterase Inhibitor-induced vascular lesions in rat[J].Toxicol Appl Res,2003,183(2):108-109.
[15]王勇,江芝婷,梁琼麟,等.代谢组学法研究三聚氰胺对儿童尿液代谢的影响[J].高等学校化学学报,2010,31(1):57-60.
[16]Kaddurah-Daouk R,Mc Evoy J,Baillie R A,et al.Metabolomic mapping of atypical antipsychotic effects inschizophrenia[J].Mol Psychiatry,2007,12(10):934-45.
[17]皮子凤,门丽慧,张静,等.五味子治疗大鼠糖尿病肾病作用机制的血清代谢组学研究[J].分析化学,2015,43(2):169-175.
[18]Clayton TA,Lindon JC,Cloarec O,et al.Pharmacometabonomic phenotyping and personalized drug treatment[J].Nature,2006,440(7087):1073-1077.
[19]曹蓓,阿基业,王广基,等.代谢组学在临床研究中的应用及进展[J].生命科学,2010,22(8):761-771.
[20]吕天,牟红元,冯江江,等.中药柴胡总皂苷急性肝毒性的代谢组学研究[J].化学研究与应用,2013,25(6):789-792.
[21]吴宏伟,李洪梅,唐力英,等.代谢组学方法研究姜黄根茎及块根次生代谢产物表达差异[J].分析化学研究报告,2012,40(5):713-717.
[22]Yongli Hua,Wenxin Xue,Man Zhang,et al.Metabonomics study on the hepatoprotective effect of polysaccharides from different preparations of Angelica sinensis[J].Journal of Ethnopharmacology,2014,151(2014)1090-1099.
[23]德洛,罗尚华,周林,等.代谢组学研究在藏医药理论及临床诊疗现代研究中的应用前景分析[Z].全国藏医药学术交流与适宜技术培训会(五藏区会议),甘肃合作市,2012.
[24]帕它木·莫合买提,哈木拉提·吾甫尔.代谢组学与维吾尔医现代研究[J].中国民族医药杂志,2008,1(1):63-65.