高效液相色谱分离分析法在葡萄酒中萃取白藜芦醇的应用
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摘要
目的提取和检测贵州地区出产的葡萄酒中的白藜芦醇,为研发高白藜芦醇葡萄酒提供理论依据,并对该种中药成分做出科学评价。方法通过高效液相色谱分离分析法分离葡萄酒中的白藜芦醇并测定其峰值,探讨该技术在白藜芦醇提取过程中的精密度、准确度和重复性。结果白藜芦醇浓度在2.50~255.00μg/m L时与峰面积呈良好的线性关系,且检测的精密度、稳定性和重复性的相对标准偏差均在0.76%以上。结论高效液相色谱新技术新方法在中药复杂体系的分离分析中具有很大的发展潜力和应用前景。
Objective Extracting and detecting resveratrol in wine production of Guizhou to provide a theoretical basis for the development of high resveratrol wine and the kinds of Chinese medicine ingredients to make a scientific evaluation.Methods Separated by HPLC separation of resveratrol in wine and determined its peak,and to explore the technology of resveratrol extraction process of precision, accuracy and repeatability. Results The results showed that when the concentration was2.50~255.00 μg/m L,the peak area showed a good linear relationship, and the relative standard deviation of the detection precision, stability and reproducibility were higher 0.76%. Conclusion A new method for high performance liquid chromatography separation and analysis of complex systems in Chinese medicine has great development potential and prospects.
Objective Extracting and detecting resveratrol in wine production of Guizhou to provide a theoretical basis for the development of high resveratrol wine and the kinds of Chinese medicine ingredients to make a scientific evaluation.Methods Separated by HPLC separation of resveratrol in wine and determined its peak,and to explore the technology of resveratrol extraction process of precision, accuracy and repeatability. Results The results showed that when the concentration was2.50~255.00 μg/m L,the peak area showed a good linear relationship, and the relative standard deviation of the detection precision, stability and reproducibility were higher 0.76%. Conclusion A new method for high performance liquid chromatography separation and analysis of complex systems in Chinese medicine has great development potential and prospects.
引文
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[15]Agati G,Azzarello E,Pollastri S,et al.Flavonoids as antioxidants in plants:location and functional significance[J].Plant Sci,2012,196:67-76.
[16]Yousefi SM,Shemirani F.Selective and sensitive speciation analysis of Cr(Ⅵ)and Cr(Ⅲ)in water samples by fiber optic-linear array detection spectrophotometry after ion pair based-surfactant assisted dispersiveliquid-liquid microextraction[J].J Hazard Mater,2013(254/255):134-140.
[2]侯丙波,冯江江,杨圣伟.白藜芦醇的合成[J].化学试剂,2011,33(12):1141-1143.
[3]李环宇,王君高,苏峰,等.葡萄酒中白藜芦醇与人体健康[J].中国酿造,2009(7):20-22.
[4]孟德平,邹志孟,许晓群,等.白藜芦醇对黑色素瘤细胞增殖的抑制作用[J].中华肿瘤防治杂志,2012,19(5):328-331.
[5]齐威.白藜芦醇可提高放射治疗效果[J].农产品加工:创新版,2012(5):30.
[6]黄卫文,李忠海,黎继烈.白藜芦醇的合成研究进展[J].中国林业科技大学学报,2010,30(10):72-75.
[7]Chen HF,Zhang WG,Yuan JB,et al.Simultaneous quantification of polymethoxylated flavones and coumarins in Fructus aurantiiand Fructus aurantii immaturus using HPLC-ESI-MS/MS[J].J Pharm Biomed Anal,2012,59:90-95.
[8]Ulusoy H,Y lmaz O,Gürkan R.A micellar im proved method for trace levels selenium quantification in food samples,alcoholicand nonalcoholic beverages through CPE/FAAS[J].Food Chem,2013,139(1/4):1008-1014.
[9]俞然,尹吉泰,邢凯,等.葡萄品种、产区及酿造工艺对酒中白藜芦醇的影响[J].中外葡萄与葡萄酒,2009(7):17-19.
[10]Ruan M,Li Y,Li X,et al.Qualitative and quanti tative analysis of the major constituents in Chinese medicinal preparation Guan-Xin-Ning injection by HPLC-DAD-ESI-MS(n)[J].J Pharm Biomed Anal,2012,59:184-189.
[11]Ndiaye M,Philippe C,Mukhtar H,et al.The grape antioxidant resveratrol for skin disorders:Promise,prospects,and challenges[J].Arch Biochem Biophys,2011,508(2):164-170.
[12]Sagratini G,Maggi F,Caprioli G,et al.Comparative study of aroma profile and phenolic content of Montepulciano mono varietal red wines from the Marches and Abruzzo regions of Italy using HS-SPME-GC-MS and HPLC-MS[J].Food Chem,2012,132(1):1592-1599.
[13]张瑞.白藜芦醇抗菌作用的研究进展[J].河北医科大学学报,2010,31(9):1151-1154.
[14]何冰,王经韵,郑宇翔.白藜芦醇抗喘活性及机制研究[J].广东药学院学报,2012,28(4):231-234.
[15]Agati G,Azzarello E,Pollastri S,et al.Flavonoids as antioxidants in plants:location and functional significance[J].Plant Sci,2012,196:67-76.
[16]Yousefi SM,Shemirani F.Selective and sensitive speciation analysis of Cr(Ⅵ)and Cr(Ⅲ)in water samples by fiber optic-linear array detection spectrophotometry after ion pair based-surfactant assisted dispersiveliquid-liquid microextraction[J].J Hazard Mater,2013(254/255):134-140.