摘要
该文以中药柴胡为材料,研究其皂苷成分对胃癌细胞MGC80-3的抑制活性,并探讨其潜在的分子机制。采用已报道的方法提取柴胡的总皂苷成分,通过显微镜观察其对MGC80-3细胞形态的影响,利用MTT法和细胞集落形成实验检测其对MGC80-3细胞增殖的影响,利用细胞黏附实验和细胞划痕实验分析其对MGC80-3细胞迁移的影响,进一步通过Western blot实验检测柴胡总皂苷对Akt/NF-κB信号通路的影响。结果显示,柴胡总皂苷显著抑制胃癌细胞MGC80-3的增殖和迁移能力,并呈现明显的量效关系和时效关系。而且,柴胡总皂苷降低了NF-κB p65的蛋白表达水平,虽然不影响Akt和IKKβ的表达,却显著抑制了二者的磷酸化水平。因此,柴胡总皂苷可能通过Akt/NF-κB信号通路发挥对胃癌细胞MGC80-3的抑制作用,具有开发为抗胃癌药物的潜在价值。
The inhibitory effect of saikosaponin from Bulperum Chinese DC on gastric cancer line MGC80-3 was accessed in this study. And then, the potential molecular mechanism was investigated. Saikosaponin was extracted from Bulperum Chinese DC using the reported method. Effect of saikosaponin on MGC80-3 cell morphology was accessed with the light microscope. Effect of saikosaponin on cell proliferation was determined by MTT method and colony formation assay. Migration capability of MGC80-3 was detected by the cell adhesive assay and wound-healing assay. Western blot was used to access the potential molecular mechanism. Saikosaponin from Bulperum Chinese DC repressed the capability of MGC80-3 cell proliferation and migration in a dose-and time-dependent manner. Saikosaponin significantly reduced the expression level of NF-κB p65, but not affect that of Akt and IKK β. Besides, phosphorylation of Akt and IKK β was decreased in cells treated by saikosaponin. Itis probable that saikosaponin from Bulperum Chinese DC repressed MGC80-3 cell via Akt/NF-κB pathway, and showed potential value in the development of anti-gastric cancer drugs.
引文
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