氯冉酸荷移分光光度法测定注射用头孢唑肟钠
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摘要
目的建立测定注射用头孢唑肟钠含量的方法。方法利用氯冉酸与头孢唑肟在乙二醇-乙醇介质中发生荷移反应,形成荷移配合物,采用分光光度法测定。结果荷移配合物在530nm处有最大吸收,表观摩尔吸收系数为1.167×10~3L/(mol·cm),药物浓度在0.005830mg·mL~(-1)～0.1895mg·mL~(-1)内符合朗伯比尔定律。回收率在98.6%～100.8%之间。结论该方法快速、准确,可用于测定药物制剂中头孢唑肟钠的含量。
OBJECTIVE To establish a method for rapid determination of Ceftizoxime sodium for injection.METHODS To utilize visible spectrophotometry based on the charge transfer reaction between Ceftizoxime sodium as donor and Chloranilic acid as acceptor in ethylene glycol-alcohol solution.RESULTS The apparent molar absorptivity of complex was 1.167×10~3L/(mol·cm) at 530 nm.Lambert-Beer law was obeyed in the range of 0.005830～0.1895 mg·mL~(-1) of Ceftizoxime sodium.The recovery of the samples was within the range of 98.6%～100.8%.CONCLUSION The proposed method is stable,rapid and accurate.It has been applied to the determination of Ceftizoxime sodium in preparation with satisfactory results.
OBJECTIVE To establish a method for rapid determination of Ceftizoxime sodium for injection.METHODS To utilize visible spectrophotometry based on the charge transfer reaction between Ceftizoxime sodium as donor and Chloranilic acid as acceptor in ethylene glycol-alcohol solution.RESULTS The apparent molar absorptivity of complex was 1.167×10~3L/(mol·cm) at 530 nm.Lambert-Beer law was obeyed in the range of 0.005830～0.1895 mg·mL~(-1) of Ceftizoxime sodium.The recovery of the samples was within the range of 98.6%～100.8%.CONCLUSION The proposed method is stable,rapid and accurate.It has been applied to the determination of Ceftizoxime sodium in preparation with satisfactory results.
引文
[1]陈新谦,金有豫,汤光.新编药物学第17版[M].北京:人民卫生出版社,2011,60.
[2]陶玲玲,朱建国.紫外分光光度法测定头孢唑肟钠粉针剂的含量[J].苏州医学院学报,1999,19(12):1294.
[3]国家药典委员会编.中国药典(二部)[S].北京:中国医药科技出版社,2015,282-283.
[4]于和,陈双璐,林澜.HPLC法测定注射用头孢唑肟钠中主药的含量[J].中国药房,2007,18(25):1981-1982.
[5]易爱纯,张顺芝,陈蒂芳.反相高效液相色谱法测定头孢唑肟钠的含量[J].中国药房,2003,14(11):687-688.
[6]张涛,周丹,赵运森.HPLC与微生物法测定注射用头孢唑肟钠含量的比较[J].现代食品与药品杂志,2007,17(1):46-48.
[7]宋健玲,谢鲜梅.荷移络合物分光光度法测定头孢噻肟钠的研究[J].光谱实验室,2011,28(1):352-356.
[8]Saleh G A,Askal H F,Raduan M F,et al.Use of Chargetransfer Complexation in the Spectrophotometric Analysis of Certain Cephalosporins[J].Talanta,2001,54(6):1205-1215.
[9]李省云.药物分析-荷移光谱法[M].北京:化学工业出版社,2008,103.
[10]李俊,闰金,王国庆,等.荷移分光光度法测定注射用头抱曲松钠[J].光谱实验室,2013,30(1):308-312.
[11]赵桐辉,李俊,李焱,等.荷移光度法测定注射用头孢唑啉钠含量[J].化学研究与应用,2014,26(9):1487-1490.
[12]田孟魁,冯喜兰,赵凤林,等.水与氯冉酸的荷移反应及其分析应用[J].分析试验室,2007,26(1):105-108.
[2]陶玲玲,朱建国.紫外分光光度法测定头孢唑肟钠粉针剂的含量[J].苏州医学院学报,1999,19(12):1294.
[3]国家药典委员会编.中国药典(二部)[S].北京:中国医药科技出版社,2015,282-283.
[4]于和,陈双璐,林澜.HPLC法测定注射用头孢唑肟钠中主药的含量[J].中国药房,2007,18(25):1981-1982.
[5]易爱纯,张顺芝,陈蒂芳.反相高效液相色谱法测定头孢唑肟钠的含量[J].中国药房,2003,14(11):687-688.
[6]张涛,周丹,赵运森.HPLC与微生物法测定注射用头孢唑肟钠含量的比较[J].现代食品与药品杂志,2007,17(1):46-48.
[7]宋健玲,谢鲜梅.荷移络合物分光光度法测定头孢噻肟钠的研究[J].光谱实验室,2011,28(1):352-356.
[8]Saleh G A,Askal H F,Raduan M F,et al.Use of Chargetransfer Complexation in the Spectrophotometric Analysis of Certain Cephalosporins[J].Talanta,2001,54(6):1205-1215.
[9]李省云.药物分析-荷移光谱法[M].北京:化学工业出版社,2008,103.
[10]李俊,闰金,王国庆,等.荷移分光光度法测定注射用头抱曲松钠[J].光谱实验室,2013,30(1):308-312.
[11]赵桐辉,李俊,李焱,等.荷移光度法测定注射用头孢唑啉钠含量[J].化学研究与应用,2014,26(9):1487-1490.
[12]田孟魁,冯喜兰,赵凤林,等.水与氯冉酸的荷移反应及其分析应用[J].分析试验室,2007,26(1):105-108.