代谢组学技术在中药药性理论研究中的应用概述
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摘要
代谢组学作为系统生物学的重要组成部分,其研究思想与中医药研究思想的整体观相似。近几年,随着代谢组学技术的蓬勃发展,其在中药现代研究中的应用越来越广泛。通过对代谢组学起源、发展、研究内容及方法进行简要概述,并对其在中药药性理论,包括四气五味、升降沉浮、归经、有毒无毒、配伍、禁忌中的应用进行概述,初步分析并总结了代谢组学应用于中药药性理论研究所存在的问题及发展趋势。
Metabonomics as an important component of systems biology,the research idea is similar to the overall concept in the research of traditional Chinese medicine(TCM).In recent years,with the vigorous development of metabonomics,its application is more and more widely in modern research of Chinese material medica(CMM).In this paper,based on the introduction of origin,development,research content,and method of metabonomics,and at the same time its application on medicinal properties in TCM theory,including four Qi,five tastes,meridian entry,direction of medicinal actions(upward,downward,outward,and inward),toxicity,compatibility of medicines,and the contraindication of application,are summarized.The preliminary analysis and sums up of research problems and development trend on the metabonomics applying in TCM property theory have been brought forward.
Metabonomics as an important component of systems biology,the research idea is similar to the overall concept in the research of traditional Chinese medicine(TCM).In recent years,with the vigorous development of metabonomics,its application is more and more widely in modern research of Chinese material medica(CMM).In this paper,based on the introduction of origin,development,research content,and method of metabonomics,and at the same time its application on medicinal properties in TCM theory,including four Qi,five tastes,meridian entry,direction of medicinal actions(upward,downward,outward,and inward),toxicity,compatibility of medicines,and the contraindication of application,are summarized.The preliminary analysis and sums up of research problems and development trend on the metabonomics applying in TCM property theory have been brought forward.
引文
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[21]刘萍,王平,陈刚,等.基于系统生物学探讨中药归经理论的科学内涵[J].中医杂志,2009,51(9):773-777.
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[26]韩亮,冯毅凡,江涛,等.蛇床子超临界提取物肝肾毒性及代谢组学的初步研究[J].中药新药与临床药理,2012,23(2):131-135.
[27]吕天,牟红元,冯江江,等.中药柴胡总皂苷急性肝毒性的代谢组学研究[J].化学研究与应用,2013,25(6):789-792.
[28]施洁瑕,马宏跃,段金廒,等.应用代谢组学探讨芫花酯甲对人肝细胞L02的毒性作用[J].中国药理学与毒理学杂志,2013,27(4):704-709.
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[30]李晶晶,王晓娟,刘妍如,等.川楝子毒性的血清代谢组学研究[J].国际药学研究杂志,2014,41(4):468-472.
[31]雷荣辉,王青秀,颜贤忠,等.利用1H-NMR技术研究大黄素染毒后大鼠内源性代谢物的改变[J].药物评价研究,2015,38(1):29-35.
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[33]邵凤,刘林生,阿基业.GC/TOF-MS代谢组学技术研究雷公藤甲素在大鼠体内的急性毒性[J].中国药科大学学报,2014,45(6):703-709.
[34]杨永霞,丁佳佳,唐冰雯,等.醋制甘遂减毒的血浆代谢组学研究[J].第二军医大学学报,2013,34(10):1097-1103.
[35]徐婷婷,金若敏,姚广涛.商陆致大鼠肾损伤的代谢组学[J].中国药理学与毒理学杂志,2013,27(3):493-494.
[36]Tan Y,Li J,Liu X,et al.Deciphering the differential toxic responses of radix aconiti lateralis praeparata in healthy and hydrocortisone-pretreated rats based on serum metabolic profiles[J].Proteome Res,2013,12(1):513-524.
[37]王喜军.中药药效物质基础研究的系统方法学——中医方证代谢组学[J].中国中药杂志,2015,40(1):13-17.
[38]王静,袁子民,李云兴,等.基于GC-MS代谢组学法研究黄连、生地黄治疗II型糖尿病的配伍机制[J].中国中药杂志,2014,39(3):526-530.
[39]周本宏,周静,吴丽宁,等.天麻-川芎对偏头痛模型大鼠尿液内源性物质代谢的影响[J].中国实验方剂学杂志,2014,20(3):104-108.
[40]谢颖,汤庆发,宋帅,等.药对麻黄-杏仁配伍对哮喘大鼠干预的尿液代谢组学研究[J].时珍国医国药,2015,26(4):815-817.
[41]施旭光,吴美音,黄曼婷,等.基于代谢组学的补中益气汤“益气升阳”配伍机制研究[J].中国实验方剂学杂志,2014,20(1):103-106.
[42]赵佳伟,何家乐,马增春,等.参附配伍减毒作用的尿液代谢组学研究[J].中药药理与临床,2015,31(1):1-5.
[43]Xu W F,Wang H F,Chen G,et al.1H-NMR-based metabonomics study on the toxicity alleviation effect of other traditional Chinese medicines in Niuhuang Jiedu tablet to realgar[J].Ethnopharmacology,2013,148(1):88-98.
[44]陈刚,徐文峰,王海峰,等.基于代谢组学的厚朴与枳实配伍减毒机制分析[J].国际药学研究杂志,2013,40(6):785-789.
[45]Wang H,Bai J,Chen G,et al.A metabolic profiling analysis of the acute hepatotoxicity and nephrotoxicity of Zhusha Anshen Wan compared with cinnabar in rats using1H-NMR spectroscopy[J].J Ethnopharmacol,2013,146(2):572-580.
[46]唐冰雯,李俊健,毋福海,等.甘遂-甘草配伍对大鼠肾脏毒性代谢组学的影响[J].中国实验方剂学杂志,2015,21(9):88-92.
[47]李俊健.基于NMR的代谢组学对甘遂甘草配伍减毒机制的研究[D].广州:广东药学院,2014.
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[2]欧阳兵,王振国,李峰,等.中药四性“性-效-物质三元论”假说及其论证[J].山东中医药大学学报,2008,32(3):172-183.
[3]张冰,翟华强,林志健,等.从“三要素”理念探讨中药药性之核心构成[J].北京中医药大学学报,2007,30(100):656-657.
[4]李石生,谭宁华.中药现代化研究的关键在于建立科学的现代中药理论体系——分子药性假说的提出[J].中国中西医结合杂志,2000,20(2):83-84.
[5]王普霞,周春祥.基于“证-药效-药性”观念及“药性本质多元”假说探讨中药药性本质[J].辽宁中医杂志,2006(2):217-218.
[6]张铁军,刘昌孝.中药五味药性理论辨识及其化学生物学实质表征路径[J].中草药,2015,46(1):1-6.
[7]Devaux P G,Horning M G,Hill R M,et al.O-Benzyloximes:Derivatives for the study of ketosteroids by gas chromatography.Application to urinary steroids of the newborn human[J].Anal Biochem,1971,41(1):70-82.
[8]Nicholson J K,Timbrell J A,Sadler P J.Proton NMR spectra of urine as indicators of renal damage.Mercury-induced nephrotoxicity in rats[J].MolPharmacol,1985,27(6):644-651.
[9]Nicholson J K,Lindon J C,Holmes E.‘Metabonomics’:understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data[J].Xenobiotica,1999,29(11):1181-1189.
[10]Fiehn O,Kopka J,Dormann P,et al.Metabolite profiling for plant functional genomics[J].Nat Biotechnol,2000,18(11):1157-1161.
[11]Fiehn O.Metabolomics—the link between genotypes and phenotypes[J].Plant Mol Biol,2002,48(1/2):155-171.
[12]Taylor J,King R D,Altmann T,et al.Application of metabolomics to plant genotype discrimination using statistics and machine learning[J].Bioinformatics,2002,18(2):241-248.
[13]崔利锐,王鹏,欧阳兵.试论中药四性理论现代研究发展趋势[J].山东中医药大学学报,2013,37(4):275-276.
[14]Kuang H X,Wang Y Y.Metabonomics research of the four properties in traditional Chinese medicine based on UPLC-QTOF-MS system[J].Chin Med,2011,11:13-28.
[15]汪娜,柳长凤,刘树民,等.基于代谢组学研究黄芩对干酵母热证模型的影响及苦寒性效关系[J].中草药,2013,44(18):2556-2562.
[16]孟宪生,姜民,罗国安,等.基于代谢组学的中药川芎对寒凝血瘀证大鼠作用机制研究[J].辽宁中医杂志,2012,39(2):218-221.
[17]钟凌云,廖智慧,龚千锋,等.基于代谢组学研究姜制对黄连药性的影响[J].中草药,2013,44(22):3177-3181.
[18]聂斌,郝竹林,桂宝,等.基于随机森林的中药寒、热药性代谢组学判别方法研究[J].江西中医药大学学报,2015,27(2):82-86.
[19]俞仲毅,韩翠翠.中药升降浮沉研究相关问题探讨[J].上海中医药大学学报,2013,27(3):7-12.
[20]韩翠翠,俞仲毅.中药升降浮沉理论沿革及应用纂要[J].上海中医药大学学报,2013,27(3):16-22.
[21]刘萍,王平,陈刚,等.基于系统生物学探讨中药归经理论的科学内涵[J].中医杂志,2009,51(9):773-777.
[22]刘昌孝,司端运,万仁忠,等.代谢组学与天然药物和中药研究[J].中国天然药物,2008,6(2):82-88.
[23]Qiu J.A culture in the balance:traditional Chinese medicine and western science face almost irreconcilable differences can systems biology bring them together?[J].Nature,2007,448(7150):126-128.
[24]刘卫红,张琪,张蕾,等.三仁汤对大鼠高脂血症模型血脂及代谢产物谱的影响[J].中国中西医结合杂志,2011,31(1):52-57.
[25]贾伟.医学代谢组学[M].上海:上海科学技术出版社,2011.
[26]韩亮,冯毅凡,江涛,等.蛇床子超临界提取物肝肾毒性及代谢组学的初步研究[J].中药新药与临床药理,2012,23(2):131-135.
[27]吕天,牟红元,冯江江,等.中药柴胡总皂苷急性肝毒性的代谢组学研究[J].化学研究与应用,2013,25(6):789-792.
[28]施洁瑕,马宏跃,段金廒,等.应用代谢组学探讨芫花酯甲对人肝细胞L02的毒性作用[J].中国药理学与毒理学杂志,2013,27(4):704-709.
[29]杨永霞,唐冰雯,丁佳佳,等.甘遂毒性的血浆代谢组学研究[J].第三军医大学学报,2014,34(1):38-41.
[30]李晶晶,王晓娟,刘妍如,等.川楝子毒性的血清代谢组学研究[J].国际药学研究杂志,2014,41(4):468-472.
[31]雷荣辉,王青秀,颜贤忠,等.利用1H-NMR技术研究大黄素染毒后大鼠内源性代谢物的改变[J].药物评价研究,2015,38(1):29-35.
[32]张盼盼,章文军,薛蓉,等.基于1H-NMR的代谢组学方法对大鼠砒霜给药后血清的研究[J].分析化学,2013,41(9):1434-1438.
[33]邵凤,刘林生,阿基业.GC/TOF-MS代谢组学技术研究雷公藤甲素在大鼠体内的急性毒性[J].中国药科大学学报,2014,45(6):703-709.
[34]杨永霞,丁佳佳,唐冰雯,等.醋制甘遂减毒的血浆代谢组学研究[J].第二军医大学学报,2013,34(10):1097-1103.
[35]徐婷婷,金若敏,姚广涛.商陆致大鼠肾损伤的代谢组学[J].中国药理学与毒理学杂志,2013,27(3):493-494.
[36]Tan Y,Li J,Liu X,et al.Deciphering the differential toxic responses of radix aconiti lateralis praeparata in healthy and hydrocortisone-pretreated rats based on serum metabolic profiles[J].Proteome Res,2013,12(1):513-524.
[37]王喜军.中药药效物质基础研究的系统方法学——中医方证代谢组学[J].中国中药杂志,2015,40(1):13-17.
[38]王静,袁子民,李云兴,等.基于GC-MS代谢组学法研究黄连、生地黄治疗II型糖尿病的配伍机制[J].中国中药杂志,2014,39(3):526-530.
[39]周本宏,周静,吴丽宁,等.天麻-川芎对偏头痛模型大鼠尿液内源性物质代谢的影响[J].中国实验方剂学杂志,2014,20(3):104-108.
[40]谢颖,汤庆发,宋帅,等.药对麻黄-杏仁配伍对哮喘大鼠干预的尿液代谢组学研究[J].时珍国医国药,2015,26(4):815-817.
[41]施旭光,吴美音,黄曼婷,等.基于代谢组学的补中益气汤“益气升阳”配伍机制研究[J].中国实验方剂学杂志,2014,20(1):103-106.
[42]赵佳伟,何家乐,马增春,等.参附配伍减毒作用的尿液代谢组学研究[J].中药药理与临床,2015,31(1):1-5.
[43]Xu W F,Wang H F,Chen G,et al.1H-NMR-based metabonomics study on the toxicity alleviation effect of other traditional Chinese medicines in Niuhuang Jiedu tablet to realgar[J].Ethnopharmacology,2013,148(1):88-98.
[44]陈刚,徐文峰,王海峰,等.基于代谢组学的厚朴与枳实配伍减毒机制分析[J].国际药学研究杂志,2013,40(6):785-789.
[45]Wang H,Bai J,Chen G,et al.A metabolic profiling analysis of the acute hepatotoxicity and nephrotoxicity of Zhusha Anshen Wan compared with cinnabar in rats using1H-NMR spectroscopy[J].J Ethnopharmacol,2013,146(2):572-580.
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