鸡新城疫病毒Ⅰ系在不同细胞上增殖特性的研究
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摘要
为了在疫苗生产过程中实现应用传代细胞系大规模增殖培养鸡新城疫病毒(Newcastle disease virus,NDV),试验将NDVⅠ系分别接种至BHK-21、Vero与DF-1细胞系中,并对F1～F3代不同细胞培养物的凝集价、细胞半数感染量(TCID_(50))与鸡胚半数感染量(EID_(50))进行测定,研究NDVⅠ系在BHK-21、Vero与DF-1细胞上的增殖特性,并确定培养NDVⅠ系的最佳细胞系。结果表明:NDVⅠ系在BHK-21、Vero与DF-1细胞中均可增殖,并产生明显的细胞病变;F1～F3代细胞培养物随着传代次数的增加,凝集价(DF-1细胞除外)、TCID_(50)与ELD_(50)下降;用BHK-21细胞培养的病毒毒力显著高于另两种细胞培养物。说明在三种传代细胞系中,BHK-21细胞较DF-1与Vero细胞更适合于NDVⅠ系的生长。
引文
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[23] JANG J,HONG S H,CHOE D,et al.Overexpression of Newcastle disease virus (NDV) V protein enhances NDV production kinetics in chicken embryo fibroblasts[J].Appl Microbiol Biotechnol,2010,85(5):1509-1520.
[2] ALEXANDER D J,ALDOUS E W,FULLER C M.et al.The long view:a selective review of 40 years of Newcastle disease research[J].Avian Pathol,2012,41(4):329-335.
[3] ALEXANDER D J.Newcastle disease in the European Union 2000 to 2009[J].Avian Pathol,2011,40(6):547-558.
[4] GANAR K,DAS M,SINHA S,et al.Newcastle disease virus:current status and our understanding[J].Virus Res,2014,184:71-81.
[5] 关平原,申之义,李平安,等.鸡新城疫Ⅰ系疫苗株Vero细胞培养物的免疫试验及Vero细胞的致瘤性检查[J].中国预防兽医学报,1999,21(4):295-297.
[6] 豆艳丽,吴润,刘磊.鸡新城疫病毒感染不同细胞的病变特性研究[J].甘肃农业大学学报,2012,47(4):1-5.
[7] WANG C,CHU Z,LIU W,et al.Newcastle disease virus V protein inhibits apoptosis in DF-1 cells by downregulating TXNL1 [J].Vet Res,2018,49(1):102-113.
[8] 曲昱蓉,詹媛,仇旭升,等.HeLa细胞中泛素蛋白酶体系统对新城疫病毒复制的影响[J].中国动物传染病学报,2014,22(2):13-19.
[9] RAVINDRA P V,TIWARI A K,RATTA B.et al.Newcastle disease virus-induced cytopathic effect in infected cells is caused by apoptosis[J].Vet Res,2009,141(1):13-20.
[10] RAVINDRA P V,TIWARI A K,RATTA B,et al.Induction of apoptosis in Vero cells by Newcastle disease virus requires viral replication,de-novo protein synthesis and caspase activation[J].Vet Res,2008,133(2):285-290.
[11] ARIFIN M A,MEL M,ABDUL KARIM M I,et al.Production of Newcastle disease virus by Vero cells grown on cytodex 1 microcarriers in a 2-litre stirred tank bioreactor[J].J Biomed Biotechnol,2010,586363:1-7.
[12] 武发菊,刘学荣,尚勇良,等.新城疫疫苗的研究进展[J].中国畜牧兽医,2011,38(10):145-148.
[13] 周晓雅,周祺,殷冬冬,等.鸭坦布苏病毒感染对BHK-21细胞分泌胞外体的影响[J].中国免疫学杂志,2018,34(2):161-166.
[14] NIKOLAY A,CASTILHO L R,REICHL U,et al.Propagation of Brazilian Zika virus strains in static and suspension cultures using Vero and BHK cells[J].Vaccine,2018,36(22):3140-3145.
[15] 武发菊,刘萍,安芳兰,等.BHK-21悬浮细胞流加培养的研究[J].中国畜牧兽医,2014,41(6):150-153.
[16] 田波,武发菊,安芳兰,等.氨和乳酸对悬浮培养的BHK-21细胞生长和胞内酶活性的影响[J].中国畜牧兽医,2011,38(9):37-40.
[17] YURCHENKO K S,ZHOU P,KOVNER A V,et al.Oncolytic effect of wild-type Newcastle disease virus isolates in cancer cell lines in vitro and in vivo on xenograft model[J].PLoS One,2018,13 (4):1-19.
[18] 古长庆,金宁一,金扩世,等.新城疫病毒在不同的细胞上增殖特性的研究[J].黑龙江畜牧兽医,2000(10):5-6.
[19] 温国元,商雨,郭静,等.新城疫病毒 TS09 耐热株在 BHK 细胞上的传代培养[J].湖北农业科学,2012,51(23):5424-5435.
[20] 韩明远,沈青春,张志刚,等.影响猪繁殖与呼吸综合征病毒感染与增殖的因素[J].动物医学进展,2010,31(1):87-91.
[21] 刘温泉,王远萍,邹年莉,等.新城疫病毒感染鸡胚成纤维细胞中免疫相关细胞因子表达的检测[J].中国家禽,2009,31(17):12-15.
[22] PARK M S,GARCIA-SASTRE A,CROS J F,et al.Newcastle disease virus V protein is a determinant of host range restriction[J].J Virol,2003,77(17):9522-9532.
[23] JANG J,HONG S H,CHOE D,et al.Overexpression of Newcastle disease virus (NDV) V protein enhances NDV production kinetics in chicken embryo fibroblasts[J].Appl Microbiol Biotechnol,2010,85(5):1509-1520.