葡萄籽提取物原花青素对奶牛肝功能和肾功能的影响
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摘要
为了研究葡萄籽提取物原花青素(GSPE)对奶牛肝脏、肾脏功能的影响,试验选取16头奶牛,按完全随机设计分成G0组(对照组)、G20组、G40组和G80组,分别按体重灌服GSPE 0,20,40,80 mg/(kg·d),每隔24 h灌服1次,持续168 h,于0(灌服前),3,6,12,24,48,72,96,120,144,168小时采血检测白蛋白(ALB)、总蛋白(TP)、球蛋白(GLOB)、尿素氮(BUN)、尿酸(UA)含量和丙氨酸氨基转移酶(ALT)、谷草转氨酶(AST)、γ-谷氨酰转肽酶(GGT)活性。结果表明:120小时,G20组ALB(估计值=4.173 9,P=0.036 1)和BUN(估计值=1.625 0,P=0.047 8)显著高于对照组,而G40组、G80组BUN均与对照组无显著差异。G20组、G40组和G80组ALT、AST、GGT、GLOB、TP和UA与对照组均无显著差异。说明按体重灌服GSPE 20~80 mg/(kg·d)对奶牛肝脏、肾脏功能指标(除BUN不确定外)未见明显不利影响。
A study was conducted to research the effects of grape seed proanthocyanidin extract( GSPE) on liver and kidney function of Holstein cattle. A completely randomized design were used and 16 cows were assigned to four groups( G0,G20,G40,and G80) and supplied each group with four different doses of GSPE: 0,20,40,and 80 mg GSPE/( kg·d). Gastric lavage technique was used every 24 hours,persisted a period of 168 hours. Blood samples were collected at 0( before drenching),3,6,12,24,48,72,96,120,144,168 h,the parameters of liver and kidney function were measured,included albumin( ALB),total protein( TP),globulin( GLOB),blood urea nitrogen( BUN),uric acid( UA),alanine aminotransferase( ALT),aspartate aminotransferase( AST),gamma glutamyl transferase( GGT). The results showed that the concentrations of ALB( Estimate = 4.173 9,P = 0.036 1) and BUN( Estimate = 1.625 0,P = 0.047 8) of G20 were significantly higher than G0,the concentrations of BUN of G40 and G80 manifested no significant difference compared with G0 at 120 hours. The concentrations of ALT,AST,GGT,GLOB,TP and UA in G20,G40 and G80 manifested no significant difference compared with G0. It was concluded that GSPE 20 ~ 80 mg GSPE/( kg·d) manifested no side effect to liver and renal function( except of BUN unclear) in this experiment.
A study was conducted to research the effects of grape seed proanthocyanidin extract( GSPE) on liver and kidney function of Holstein cattle. A completely randomized design were used and 16 cows were assigned to four groups( G0,G20,G40,and G80) and supplied each group with four different doses of GSPE: 0,20,40,and 80 mg GSPE/( kg·d). Gastric lavage technique was used every 24 hours,persisted a period of 168 hours. Blood samples were collected at 0( before drenching),3,6,12,24,48,72,96,120,144,168 h,the parameters of liver and kidney function were measured,included albumin( ALB),total protein( TP),globulin( GLOB),blood urea nitrogen( BUN),uric acid( UA),alanine aminotransferase( ALT),aspartate aminotransferase( AST),gamma glutamyl transferase( GGT). The results showed that the concentrations of ALB( Estimate = 4.173 9,P = 0.036 1) and BUN( Estimate = 1.625 0,P = 0.047 8) of G20 were significantly higher than G0,the concentrations of BUN of G40 and G80 manifested no significant difference compared with G0 at 120 hours. The concentrations of ALT,AST,GGT,GLOB,TP and UA in G20,G40 and G80 manifested no significant difference compared with G0. It was concluded that GSPE 20 ~ 80 mg GSPE/( kg·d) manifested no side effect to liver and renal function( except of BUN unclear) in this experiment.
引文
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[6]郝瑞荣,高俊杰,王伟伟,等.高粱原花青素对断奶仔猪生长性能和免疫力及抗氧化功能的影响[J].中国畜牧杂志,2015,51(13):66-70.
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[13]王卫娜.葡萄籽原花青素对糖尿病大鼠肾脏GLUT2及肾功能的影响[J].中医临床研究,2016,8(11):10-12.
[14]INGVARTSEN K L.Feeding-and management-related diseases in the transition cow:Physiological adaptations around calving and strategies to reduce feeding-related diseases[J].Anim Feed Sci Tech,2006,126(3):175-213.
[15]潘细妹,聂培,陈雯雯,等.广西奶牛产后肝功能指标AST、GGT动态监测[J].广西畜牧兽医,2011,27(3):140-141.
[16]贺绍君,赵书景,李静,等.加味益母散对围产期奶牛生产性能及肝功能的影响[J].中国兽医杂志,2015,51(11):67-69.
[17]PATRA A K,SAXENA J.Exploitation of dietary tannins to improve rumen metabolism and ruminant nutrition[J].J Sci Food Agric,2011,91(1):24-37.
[18]何宝祥,马径军,张宇,等.围产期奶牛肾功能的动态变化[J].中国兽医杂志,2009,45(8):45-46.
[19]陈会丛,翟建英,张广平,等.葡萄籽提取物原花青素一般药理学研究[J].中国中医药信息杂志,2014,21(3):36-39.
[20]陈会丛,翟建英,张广平,等.葡萄籽原花青素的毒理学研究[J].食品工业科技,2014,35(2):317-323.
[21]WREN A F,CLEARY M,FRANTZ C,et al.90-day oral toxicity study of a grape seed extract(ih636)in rats[J].J Agric Food Chem,2002,50(7):2180-2192.
[22]何杰颖.原花青素对氨基脲染毒小鼠肝肾损伤的影响[D].衡阳:南华大学,2011.
[23]CORREDDU F,NUDDA A,MANCA M G,et al.Light-induced lipid oxidation in sheep milk:Effects of dietary grape seed and linseed,alone or in combination,on milk oxidative stability[J].J Agric Food Chem,2015,63(15):3980-3986.
[2]GESSNER D K,KOCH C,ROMBERG F J,et al.The effect of grape seed and grape marc meal extract on milk performance and the expression of genes of endoplasmic reticulum stress and inflammation in the liver of dairy cows in early lactation[J].J Dairy Sci,2015,98(12):8856-8868.
[3]郝瑞荣,高俊杰,高文伟,等.原花青素的饲用价值研究进展[J].饲料广角,2015(8):39-41.
[4]BAGCHI D,SWAROOP A,PREUSS H G,et al.Free radical scavenging,antioxidant and cancer chemoprevention by grape seed proanthocyanidin:an overview[J].Mutat Res,2014,768:69-73.
[5]刘国安,冯筱璐,牛娟,等.原花青素抗氧化活性及对细胞氧化损伤的保护作用[J].西北师范大学学报(自然科学版),2011,47(6):58-62.
[6]郝瑞荣,高俊杰,王伟伟,等.高粱原花青素对断奶仔猪生长性能和免疫力及抗氧化功能的影响[J].中国畜牧杂志,2015,51(13):66-70.
[7]SERRA T A,ROCHA J,SEPODES B,et al.Evaluation of cardiovascular protective effect of different apple varieties:Correlation of response with composition[J].Food Chem,2012,135(4):2378-2386.
[8]CASTELL-AUVA,CEDL,PALLARS V,et al.Procyanidins modify insulinemia by affecting insulin production and degradation[J].J Nutr Biochem,2012,23(12):1565-1572.
[9]KRONBERG S L,SCHAUER C S.Cattle and sheep develop preference for drinking water containing grape seed tannin[J].Animal,2013,7(10):1714-1720.
[10]CHEDEA V S,BRAICU C,CHIRIF,et al Antibacterial action of an aqueous grape seed polyphenolic extract[J].Afr J Biotechnol,2011,10(33):6276-6280.
[11]文静.原花青素对瘦肉精致小鼠肝肾损伤的影响[D].衡阳:南华大学,2014.
[12]姜婧,陈雁,张海莉,等.原花青素对铅诱导大鼠肝损伤的保护作用[J].中国兽医科学,2016,46(5):664-670.
[13]王卫娜.葡萄籽原花青素对糖尿病大鼠肾脏GLUT2及肾功能的影响[J].中医临床研究,2016,8(11):10-12.
[14]INGVARTSEN K L.Feeding-and management-related diseases in the transition cow:Physiological adaptations around calving and strategies to reduce feeding-related diseases[J].Anim Feed Sci Tech,2006,126(3):175-213.
[15]潘细妹,聂培,陈雯雯,等.广西奶牛产后肝功能指标AST、GGT动态监测[J].广西畜牧兽医,2011,27(3):140-141.
[16]贺绍君,赵书景,李静,等.加味益母散对围产期奶牛生产性能及肝功能的影响[J].中国兽医杂志,2015,51(11):67-69.
[17]PATRA A K,SAXENA J.Exploitation of dietary tannins to improve rumen metabolism and ruminant nutrition[J].J Sci Food Agric,2011,91(1):24-37.
[18]何宝祥,马径军,张宇,等.围产期奶牛肾功能的动态变化[J].中国兽医杂志,2009,45(8):45-46.
[19]陈会丛,翟建英,张广平,等.葡萄籽提取物原花青素一般药理学研究[J].中国中医药信息杂志,2014,21(3):36-39.
[20]陈会丛,翟建英,张广平,等.葡萄籽原花青素的毒理学研究[J].食品工业科技,2014,35(2):317-323.
[21]WREN A F,CLEARY M,FRANTZ C,et al.90-day oral toxicity study of a grape seed extract(ih636)in rats[J].J Agric Food Chem,2002,50(7):2180-2192.
[22]何杰颖.原花青素对氨基脲染毒小鼠肝肾损伤的影响[D].衡阳:南华大学,2011.
[23]CORREDDU F,NUDDA A,MANCA M G,et al.Light-induced lipid oxidation in sheep milk:Effects of dietary grape seed and linseed,alone or in combination,on milk oxidative stability[J].J Agric Food Chem,2015,63(15):3980-3986.