芪黄扶正颗粒提取纯化工艺研究
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摘要
目的优化芪黄扶正颗粒的提取纯化工艺。方法以马钱苷、芍药苷、阿魏酸提取率、指纹图谱特征峰峰面积、干浸膏得率等为评价指标,考察乙醇浓度、药液比、提取时间、提取次数对芪黄扶正颗粒提取工艺的影响;以马钱苷、芍药苷、阿魏酸转移率、指纹图谱特征峰峰面积、干浸膏得率等为综合评价指标,考察加样顺序、药液乙醇含量、药液浓度、絮凝剂用量对芪黄扶正颗粒纯化工艺的影响。结果最佳提取工艺为:乙醇浓度60%,乙醇8倍用量,每次提取1.5h,提取2次;最佳纯化工艺为:絮凝剂中B组分先加,A组分后加,药液乙醇含量30%,药液浓度0.1409g·ml~(-1),絮凝剂中B组分用量4%,A组分2%。结论优化的提取纯化工艺稳定、可靠,为获得有效和低给药剂量的芪黄扶正颗粒剂奠定了试验基础。
Objective To optimize the extraction and purification process of Qihuang Fuzhengparticles.Methods The concentration and amount of ethanol,extraction time,and the number of times of extraction were investigated to optimize the extraction process of Qihuang Fuzhengparticles with the extraction rate of loganin,paeoniflorin,and ferulic acid,dry extract yield,and fingerprint characteristic peak area score as evaluation indexes.The effect of the order in which samples were added,ethanol concentration in the concentrated solution,concentration of extracting solution,and flocculant agent amount on the purification process was also investigated.Results The best extraction technology was optimized as follows:60% ethanol,eight times the ethanol volume,extraction twice,each time for 1.5 h.The best purification technology was optimized as follows:flocculant agent B was added before A agent,30% ethanol in the concentrated solution,0.1409 g·ml~(-1) of the concentration of the extraction liquid,4% flocculant agent B and 2% agent A.Conclusion The optimized extraction and purification process of Qihuang Fuzheng particles is stable and reliable,which will help produce effective and low-dosage Qihuang Fuzhengparticles.
Objective To optimize the extraction and purification process of Qihuang Fuzhengparticles.Methods The concentration and amount of ethanol,extraction time,and the number of times of extraction were investigated to optimize the extraction process of Qihuang Fuzhengparticles with the extraction rate of loganin,paeoniflorin,and ferulic acid,dry extract yield,and fingerprint characteristic peak area score as evaluation indexes.The effect of the order in which samples were added,ethanol concentration in the concentrated solution,concentration of extracting solution,and flocculant agent amount on the purification process was also investigated.Results The best extraction technology was optimized as follows:60% ethanol,eight times the ethanol volume,extraction twice,each time for 1.5 h.The best purification technology was optimized as follows:flocculant agent B was added before A agent,30% ethanol in the concentrated solution,0.1409 g·ml~(-1) of the concentration of the extraction liquid,4% flocculant agent B and 2% agent A.Conclusion The optimized extraction and purification process of Qihuang Fuzheng particles is stable and reliable,which will help produce effective and low-dosage Qihuang Fuzhengparticles.
引文
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[2]张星海,李嬛,秦昆明,等.多指标正交试验优化菊花提取工艺[J].中国中药杂志,2013,38(6):821
[3]汪小涵,沈成英,庞建云,等.楮桃叶配方颗粒的制备工艺及质量标准研究[J].解放军药学学报,2016,32(6):502-504
[4]张婷,杨晓旭,高家荣,等.基于超高效液相色谱指纹图谱的正交试验优选“二神丸”药对醇提工艺考察[J].中国医院药学杂志,2015,35(12):1130
[5]马文娜,程雪梅,马桂芝,等.多指标综合评价法优化复方石淋通胶囊的提取工艺研究[J].上海中医药杂志,2016,50(4):88
[6]柯小温,陈磊,宋洪涛,等.大孔树脂与ZTC联用纯化白子草总黄酮的研究[J].中国中药杂志,2012,37(9):1219
[7]曾令军,柯小温,宋洪涛.ZTC与大孔树脂联用纯化白子草总多糖的研究[J].时珍国医国药,2016,27(7):1637
[8]张萍,杨燕,鄢丹,等.多指标成分含量测定与指纹图谱分析在中药制备工艺与质量控制中的应用[J].中华中医药杂志,2010,25(1):120-123
[9]殷明阳,刘素香,张铁军,等.复方中药提取工艺研究概况[J].中草药,2015,46(21):3279-3283