由美国进境生菜种子上番茄斑萎病毒的检测鉴定
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摘要
采用DAS-ELISA检测方法,从美国进境的生菜种子中筛选出携带有番茄斑萎病毒(Tomato spotted wilt virus,TSWV)的疑似阳性样品,通过建立的RT-PCR方法对酶联免疫吸附测定阳性样品进行复核鉴定,并进一步测序。测序结果与GenBank中已有的番茄斑萎病毒分离物KT160282、AY744474、GU369726、KP330470的序列同源性达到96%。结果表明,TSWV可通过种子携带入境。
Tomato spotted will virus(TSWV) were detected in lettuce seeds from America by using DAS-ELISA.The samples were tested by RT-PCR and the products were sequenced.BLAST results showed that it had 96%identity with TSWV isolates from Genhank,and Genbank accession number were KT160282,AY744474,GU369726 and KP330470.The study indicated that TSWV can be transmitted by seeds.
Tomato spotted will virus(TSWV) were detected in lettuce seeds from America by using DAS-ELISA.The samples were tested by RT-PCR and the products were sequenced.BLAST results showed that it had 96%identity with TSWV isolates from Genhank,and Genbank accession number were KT160282,AY744474,GU369726 and KP330470.The study indicated that TSWV can be transmitted by seeds.
引文
[1]Moreno A,Bias C D,Biurrun R.The incidence and distribution of viruses infecting lettuce,cultivated Brassica and associated natural vegetation in Spain[J].Annals of Applied Biology,2004,144(3):339-346.
[2]Soleimani P,Mosahebi G,Habibi M K.Identification of some viruses causing mosaic on lettuce and characterization of Lettuce mosaic virus from Tehran province in Iran[J].African Journal of Agricultural Research,2011,6(13):3029-3035.
[3]夏俊强.莴苣病毒国外研究概述[J].中国蔬菜,1987,26(4):44-47.
[4]Kamberoglu M A,Alan B.Occurrence of Tomato spotted wilt virus in lettuce in Cukurova Region of Turkey[J].International Journal of Agriculture&Biology,2011,13(3):431-434.
[5]Wilson C R.Incidence of weed reservoirs and vectors of Tomato spotted wilt virus on southern Tasmanian lettuce farms[J].Plant Pathology,1998,47(2):171-176.
[6]Pappu H,Jones R,Jain R.Global status of tospovirus epidemics in diverse cropping systems:Successes achieved and challenges ahead[J].Virus Research,2009,141:219-236.
[7]Al-Saleh M A,Al-Shahwan I M,Amer MA,et al.First report of Tomato spotted wilt virus inlettuce crops in Saudi Arabia[J].Plant Disease,2014,98(11):1591.
[3]夏俊强.莴苣病毒国外研究概述[J].中国蔬菜,蔬菜的侵染危害[J].植物保护,2015,41(5):174-178.
[9]Pattella G,Gogalons P,Gebre S K,et al.An update o f the host range of Tomato spotted wilt virus[J].Journal of Plant Pathology,2003,85:227-264.
[10]Jones D R.Plant viruses transmitted by thrips[J].European Journal of Plant Pathology,2005,113:119-157.
[11]Kamberoglu M A,Alan B.Occurrence of Tomato spotted wilt virus in lettuce in Cukurova region of Turkey[J].Journal of Agrkmlture&Biology,2011,13:431-434.
[12]Everth E E R,Rocio A A,Olga Y M.et al.Tomato spotted wilt virus(TSWV),weeds and thrip vectors in the tomato(Solanum lycopersicum L.)in the Andean region of Cundinamarca(Colombia)[J].Agronomia Colombiana,2013,31(1):58-67.
[13]Coutts B A,Thomas-Carroll M L,Jones R A C.Patterns of spread of Tomato spotted wilt virus in field crops of lettuce and pepper:spatial dynamics and validation of control measures[J].Annals of Applied Biology,2004,145(2):231-245.
[14]Morris J.Diagnostic protocols for regulated pests:Tomato spotted wilt tospovirus,impatiens necrotic spot tospovirus and watermelon silver mottle tospovirus[R].Bulletin OEPP/EPPO Bulletin,2004,34(2):271-279.
[15]Crowley N C.Studies on the seed transmission of plant virus diseases[J].Australian Journal of Biological Sciences,1957,10(4):449-464.
[16]Pappu S S,Pappu H R,Culbreath A K.et al.Localization of Tomatospotted wilt virus(Genus Tospovirus,Family Bunyaviridae)inpeanut pods[J].Peanut Science,1999,26(2):98-100.
[17]冯黎霞,章柱.从美国进境生菜种子中首次截获番茄斑萎病毒[J].植物检疫,2013,27(1):57.
[18]佟爱仔,赵兴能,孔宝华,等.侵染云南烟草的番茄斑萎病毒(TSWV)的RT-PCR检测[J].云南农业大学学报,2012,27(6):809-813.
[19]丁铭,张丽珍,方琦,等.侵染马铃薯的一个Tospovius混合分离物的鉴定、纯化及多抗血清制备[J].西南农业学报,2004,17(S):160-162.
[20]方琦,董家红,丁铭,等.侵染水鬼蕉和花朱顶红的番茄斑萎病毒属病毒的电镜和DAS-ELISA诊断[J].园艺学报,2011,38(10):2005-2009.
[21]邱树亮,王孝宣,杜永臣.番茄斑萎病毒TSWV的鉴定及抗病种质的筛选[J].园艺学报,2012,39(6):1107-1114.
[2]Soleimani P,Mosahebi G,Habibi M K.Identification of some viruses causing mosaic on lettuce and characterization of Lettuce mosaic virus from Tehran province in Iran[J].African Journal of Agricultural Research,2011,6(13):3029-3035.
[3]夏俊强.莴苣病毒国外研究概述[J].中国蔬菜,1987,26(4):44-47.
[4]Kamberoglu M A,Alan B.Occurrence of Tomato spotted wilt virus in lettuce in Cukurova Region of Turkey[J].International Journal of Agriculture&Biology,2011,13(3):431-434.
[5]Wilson C R.Incidence of weed reservoirs and vectors of Tomato spotted wilt virus on southern Tasmanian lettuce farms[J].Plant Pathology,1998,47(2):171-176.
[6]Pappu H,Jones R,Jain R.Global status of tospovirus epidemics in diverse cropping systems:Successes achieved and challenges ahead[J].Virus Research,2009,141:219-236.
[7]Al-Saleh M A,Al-Shahwan I M,Amer MA,et al.First report of Tomato spotted wilt virus inlettuce crops in Saudi Arabia[J].Plant Disease,2014,98(11):1591.
[3]夏俊强.莴苣病毒国外研究概述[J].中国蔬菜,蔬菜的侵染危害[J].植物保护,2015,41(5):174-178.
[9]Pattella G,Gogalons P,Gebre S K,et al.An update o f the host range of Tomato spotted wilt virus[J].Journal of Plant Pathology,2003,85:227-264.
[10]Jones D R.Plant viruses transmitted by thrips[J].European Journal of Plant Pathology,2005,113:119-157.
[11]Kamberoglu M A,Alan B.Occurrence of Tomato spotted wilt virus in lettuce in Cukurova region of Turkey[J].Journal of Agrkmlture&Biology,2011,13:431-434.
[12]Everth E E R,Rocio A A,Olga Y M.et al.Tomato spotted wilt virus(TSWV),weeds and thrip vectors in the tomato(Solanum lycopersicum L.)in the Andean region of Cundinamarca(Colombia)[J].Agronomia Colombiana,2013,31(1):58-67.
[13]Coutts B A,Thomas-Carroll M L,Jones R A C.Patterns of spread of Tomato spotted wilt virus in field crops of lettuce and pepper:spatial dynamics and validation of control measures[J].Annals of Applied Biology,2004,145(2):231-245.
[14]Morris J.Diagnostic protocols for regulated pests:Tomato spotted wilt tospovirus,impatiens necrotic spot tospovirus and watermelon silver mottle tospovirus[R].Bulletin OEPP/EPPO Bulletin,2004,34(2):271-279.
[15]Crowley N C.Studies on the seed transmission of plant virus diseases[J].Australian Journal of Biological Sciences,1957,10(4):449-464.
[16]Pappu S S,Pappu H R,Culbreath A K.et al.Localization of Tomatospotted wilt virus(Genus Tospovirus,Family Bunyaviridae)inpeanut pods[J].Peanut Science,1999,26(2):98-100.
[17]冯黎霞,章柱.从美国进境生菜种子中首次截获番茄斑萎病毒[J].植物检疫,2013,27(1):57.
[18]佟爱仔,赵兴能,孔宝华,等.侵染云南烟草的番茄斑萎病毒(TSWV)的RT-PCR检测[J].云南农业大学学报,2012,27(6):809-813.
[19]丁铭,张丽珍,方琦,等.侵染马铃薯的一个Tospovius混合分离物的鉴定、纯化及多抗血清制备[J].西南农业学报,2004,17(S):160-162.
[20]方琦,董家红,丁铭,等.侵染水鬼蕉和花朱顶红的番茄斑萎病毒属病毒的电镜和DAS-ELISA诊断[J].园艺学报,2011,38(10):2005-2009.
[21]邱树亮,王孝宣,杜永臣.番茄斑萎病毒TSWV的鉴定及抗病种质的筛选[J].园艺学报,2012,39(6):1107-1114.